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Invitrogen
alpha-1 Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody (HL114)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
alpha-1 Sodium Potassium ATPase Antibody (MA5-36259) in ICC/IF
Immunofluorescence analysis of ATP1A1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with alpha-1 Sodium Potassium ATPase Monoclonal Antibody (HL114) (Product # MA5-36259) at 1:100 dilution in 0.1% ... View More
Product Details
MA5-36259
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL114
Immunogen
The immunogen used to generate this antibody corresponds to chicken Sodium/Potassium ATPase alpha 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890413
Product Specific Information
Positive control: A431 , A431 membrane extract.
Target Information
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (Na+ + K+)-ATPase; ATP1B; ATPase; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium-transporting ATPase subunit alpha-1
Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b
UniProt ID: (Human) P05023, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2
Entrez Gene ID: (Human) 476, (Chicken) 396530, (Rat) 24211, (Mouse) 11928
sodium:potassium-exchanging ATPase activity
protein binding
ATP binding
phosphatase activity
protein kinase binding
protein domain specific binding
ankyrin binding
potassium ion binding
sodium ion binding
ADP binding
phosphatidylinositol 3-kinase binding
chaperone binding
steroid hormone binding
metal ion binding
nucleotide binding
hydrolase activity
primary active transporter
regulation of the force of heart contraction
regulation of sodium ion transport
cellular sodium ion homeostasis
regulation of blood pressure
potassium ion import
establishment or maintenance of transmembrane electrochemical gradient
ATP hydrolysis coupled proton transport
dephosphorylation
cellular potassium ion homeostasis
negative regulation of glucocorticoid biosynthetic process
ion transmembrane transport
sodium ion export from cell
response to drug
negative regulation of heart contraction
positive regulation of heart contraction
positive regulation of striated muscle contraction
relaxation of cardiac muscle
cardiac muscle contraction
membrane hyperpolarization
cellular response to mechanical stimulus
cellular response to steroid hormone stimulus
regulation of cardiac muscle cell contraction
membrane repolarization
membrane repolarization during cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
response to glycoside
regulation of cardiac conduction
potassium ion import across plasma membrane
sodium ion transport
potassium ion transmembrane transport
potassium ion transport
energy coupled proton transmembrane transport, against electrochemical gradient
sodium ion transmembrane transport
transport
ion transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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