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Product Details
RAM-A2M
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse alpha-2 macroglobulin
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: Bovine ± ferret ± Horse ± Rat ++ Cat ± Goat - Human -Sheep - Chicken - Guinea Pig ± Monkey - Swine ± Dog - Hamster + pigeon - Turkey -.
In immunoelectrophoresis use 2 µL serum or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter centre well and 2 µL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Target Information
Alpha 2-Macroglobulin (a2M), is a approximately 720-kDa homotetrameric glycoprotein composed of four identical approximately 180 kDa subunits. aAlpha 2M shares with other alpha-macroglobulins, like the complement components C3 and C4 and the pregnancy zone protein PZP, an extraordinary binding capacity for a variety of ligands. This allows the alpha-macroglobulins to serve as humoral defense barriers against foreign peptides in the plasma. Alpha 2M interacts and captures virtually any proteinase, often referred to as a panprotease inhibitor. In the brain of Alzheimer's disease (AD) patients, Alpha 2M also has been localized to diffuse amyloid plaques, supporting an important role for alpha 2M in AD etiopathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2mp; Alpha 2 M; Alpha-2-M (A2M); Alpha-2-macroglobulin; Alpha-2-macroglobulin-P; DKFZp779B086; S863 7
Gene Aliases: A2m; A2mp
UniProt ID: (Mouse) Q6GQT1
Entrez Gene ID: (Mouse) 232345
protease binding
endopeptidase inhibitor activity
serine-type endopeptidase inhibitor activity
receptor binding
protein binding
growth factor binding
enzyme binding
interleukin-8 binding
interleukin-1 binding
peptidase inhibitor activity
tumor necrosis factor binding
calcium-dependent protein binding
protease inhibitor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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