Invitrogen

alpha Galactosidase Polyclonal Antibody

7 Published Figures

View all (25) alpha Galactosidase antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)
Published Figures (7)

alpha Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

alpha Galactosidase Antibody (PA5-27349) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha (Product # PA5-27349) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

alpha Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Immunocytochemistry (ICC/IF)

alpha Galactosidase Antibody in Immunocytochemistry (ICC/IF)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

alpha Galactosidase Antibody in Western Blot (WB)

Product Image Gallery

alpha Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha (Product # PA5-27349) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

alpha Galactosidase Antibody in Western Blot (WB) — Western Blot using alpha Galactosidase Polyclonal Antibody (Product # PA5-27349). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. alpha Galactosidase Polyclonal Antibody. alpha Galactosidase Polyclonal Antibody (Product # PA5-27349) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

alpha Galactosidase Antibody in Western Blot (WB) — alpha Galactosidase Polyclonal Antibody detects Galactosidase alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Galactosidase Polyclonal Antibody (Product # PA5-27349) at a dilution of 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

alpha Galactosidase Antibody in Western Blot (WB) — alpha Galactosidase Polyclonal Antibody detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Galactosidase Polyclonal Antibody (Product # PA5-27349) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

alpha Galactosidase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ex vivo-expanded porcine ACFCs epigenomically modulated (TSA+) ( b , d , f ) and not modulated with trichostatin A (TSA-) ( a , c , e ). Representative microphotographs of immunofluorescence localization of human leukocyte antigen-E (HLA-E; ( a , b )), recombinant human alpha1,2-fucosyltransferase (rhalpha1,2-FT; ( c , d )) and alpha-galactosidase A (rhalpha-Gal A; ( e , f )) in ACFCs originating from h FUT2 xh GLA x HLA-E triple-transgenic pigs. Immunofluorescent staining with Alexa Fluor 488- or Cy3-labelled secondary antibodies (green and red fluorescence, respectively) and DAPI counterstaining (blue fluorescence). Scale bars represent 100 mum. Immunoreaction was performed on ex vivo proliferating porcine ACFCs derived from at least three pigs of each experimental group. The immunofluorescence signal arisen from rhalpha1,2-FT was distributed in the perinuclear region of all the analyzed ACFCs from each variant. The MHC representative termed as HLA-E and rhalpha-Gal A enzyme were homogeneously located in whole cytoplasm of all the analyzed ACFCs. The TSA + ACFCs were characterized by largely intensified signal estimated for both HLA-E and rhalpha-Gal A proteins ( b , f ), but not for rhalpha1,2-FT ( d ) as compared to their TSA - cell counterparts ( a , c , e ).

alpha Galactosidase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of the ex vivo-expanded porcine ACFCs epigenomically modulated (TSA+) ( b , d , f ) and not modulated with trichostatin A (TSA-) ( a , c , e ). Representative microphotographs of immunofluorescence localization of swine homolog of human leukocyte antigen-E (shHLA-E; ( a , b )), alpha1,2-fucosyltransferase (alpha1,2-FT; ( c , d )) and alpha-galactosidase A (alpha-Gal A; ( e , f )) in ACFCs stemming from non-transgenic pigs (CTR nTG). Immunofluorescent staining with Alexa Fluor 488- or Cy3-labelled secondary antibodies (green and red fluorescence, respectively) and DAPI counterstaining (blue fluorescence). Scale bars represent 100 mum. Immunoreaction was performed on ex vivo proliferating porcine ACFCs derived from at least three pigs of each experimental group. TSA + ACFCs displayed more highly intensified signals identified for not only shHLA-E but also alpha1,2-FT and alpha-Gal A proteins in control non-transgenic (CTR nTG) ACFCs ( b , d , f ). No positive signals originating from either shHLA-E representative of MHC or alpha1,2-FT and alpha-Gal A enzymes were observed in the TSA - CTR nTG group ( a , c , e ).

alpha Galactosidase Antibody in Western Blot (WB) — Western blot analysis of the relative expression of human alpha1,2-fucosyltransferase (alpha1,2-FT), alpha-galactosidase A (alpha-Gal A) and human leukocyte antigen-E (HLA-E) proteins in porcine triple-transgenic and non-transgenic ACFCs epigenetically transformed or not transformed with trichostatin A (TSA + and TSA - , respectively). Representative blots of the expression of alpha1,2-FT, alpha-Gal A and HLA-E proteins in the ACFC samples derived from epigenomically modified (TSA + ) and non-modified (TSA - ) groups--panel ( A ). The samples stemming from non-transgenic pigs served as a control group (CTR nTG)--panel ( A ). beta-Actin provided a loading control for all the analyzed samples. The results of relative expression (in arbitrary units) of alpha1,2-FT, alpha-Gal A and HLA-E or swine homolog of HLA-E (shHLA-E) are shown in panels ( B - D ), respectively. The relative optical density (ROD) from three separate analyses of at least three animals for each variant is expressed as mean. The bar graphs show the mean +- SEM. Statistics: one-way ANOVA and Newman-Keuls post hoc test. The bars marked with different letters vary significantly; values denoted as a-b, b-c, e-g: p < 0.01; a-c, d-e, e-g, f-g, h-i, h-j, i-j: p < 0.05.

alpha Galactosidase Antibody in Western Blot (WB) — Ambroxol is a PC-enhancer. IF-GLA-C56Y ( A ), IF-GLA-L300F ( B ), and IF-GLA-D244H ( C ) were treated for 72 h with the following drugs: i. untreated; ii. 10 muM DGJ; iii. 40 muM ambroxol (ABX); iv. 10 muM DGJ + 40 muM ABX. AGAL specific activity measured on protein extracts is shown. Tukey's HSD was used to evaluate significative differences among treatments (***: p < 0.001 n = 3). The effects of combined treatment (DGJ + ABX) are significantly larger than those of DGJ monotherapy in all the mutants except for D244H. Immunoblots confirmed the results (U = untreated; D = DGJ 10 muM; ABX = ambroxol 40 muM; D + ABX = DGJ 10 muM + ambroxol 40 muM). Arrows on the immunoblot in panel A highlight the higher molecular weight band (precursor) and the lower molecular weight band (active form) of AGAL. IF-NULL was used as a negative control ( D ). Each panel includes specific activity and an immunoblot for a cell line.

alpha Galactosidase Antibody in Western Blot (WB) — Acetylsalicylic acid is a PC-enhancer. IF-GLA-MUTs, specifically C56Y ( A ), L300F ( B ), D244H ( C ), V269M ( D ), and Q280K ( E ), were treated for 72 h with the following drugs: i. untreated; ii. 10 muM DGJ; iii. 4 mM acetylsalicylic acid (ASA); iv. 10 muM DGJ + 4 mM ASA. IF-NULL was used as a control ( F ). AGAL specific activity measured on protein extracts is shown. Tukey's HSD was used to evaluate significative differences among treatments (***: p < 0.001; **: p < 0.01; *: p < 0.05; n = 2). The effects of combined treatment (DGJ + ASA) are significantly higher than those of DGJ monotherapy. Immunoblots confirmed the results (U = untreated; D = DGJ 10 muM; A = ASA 4 mM; D + A = DGJ 10 muM + ASA 4 mM). Each panel includes specific activity and an immunoblot for a cell line.

alpha Galactosidase Antibody in Western Blot (WB) — Figure 3 Western blot-mediated estimation of the semiquantitative profiles noticed for expression of recombinant human alpha1,2-fucosyltransferase (rhalpha1,2-FT) and alpha-galactosidase A (rhalpha-Gal A) enzymes in total protein samples extracted from the ex vivo-expanded control nontransgenic (CTR nTG) PEKs and their cell counterparts stemming from h FUT2 and h GLA single-transgenic pigs and h FUT2 xh GLA double-transgenic specimens. Representative blots corresponding to expression of rhalpha1,2-FT and rhalpha-Gal A enzymes in total protein samples that were isolated from PEKs stemming from h FUT2 mono-transgenic ( a ), h GLA mono-transgenic ( b ), h FUT2 xh GLA bi-transgenic ( c ) and CTR nTG pigs ( d ). beta-Actin provides a loading control for all analyzed protein samples. Results of the semiquantitatively analyzing the relative abundances (RAs) determined for rhalpha1,2-FT and rhalpha-Gal A enzymes (in arbitrary units) were presented in panels ( e , f ), respectively. Bar graphs show mean +- standard error of mean (SEM) of relative optical density (ROD) descended from three separate analyses of three animals for each variant. Red line is taken as the cut-off value 1.0. Statistics: one-way analysis of variance (ANOVA) followed by Tukey's honestly significant difference (HSD) post hoc test. Values that are denoted as ** and *** indicate incidence of statistically significant differences between experimental groups with a probability of occurring random errors at the level

alpha Galactosidase Antibody in Western Blot (WB) — Figure 1 Western blot analysis of the relative expression of human alpha1,2-fucosyltransferase (alpha1,2-FT) and alpha-galactosidase A (alpha-Gal A) proteins in porcine bi-transgenic and non-transgenic adult cutaneous fibroblast cells (ACFCs) undergoing or not undergoing treatment with trichostatin A (TSA + and TSA - , respectively). Representative blots of the expression of alpha1,2-FT and alpha-Gal A proteins in the ACFC samples derived from epigenetically modulated (TSA + ) and non-modulated (TSA - ) cells. The samples of non-transgenic animals served as a control group (CTR nTG--panel ( A )). beta-Actin served as a loading control for all analyzed samples. The results of relative expression (in arbitrary units) of alpha1,2-FT and alpha-Gal A are shown in panels ( B , C ), respectively. Relative optical density (ROD) from three separate analyses of at least three animals for each variant is expressed as mean. Bar graphs show mean +- standard error of the mean (SEM). The red line is taken as the cut-off value 1.0. Statistics: One-way ANOVA and Tukey's honestly significant difference (HSD) post hoc test. The bars marked with different letters differ significantly; values denoted as a-b, a-d, b-c, b-d, e-f: p < 0.01; a-c, c-d, e-g, f-g: p < 0.05.

Product Details

PA5-27349

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

- -

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 289 of Human Galactosidase alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.157 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544825

Product Specific Information

Recommended positive controls: 293T, HeLa, Rat Lung.

Predicted reactivity: Rat (82%), Pig (84%), Bovine (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Curcumin Has Beneficial Effects on Lysosomal Alpha-Galactosidase: Potential Implications for the Cure of Fabry Disease.

International journal of molecular sciences

Monticelli M,Hay Mele B,Allocca M,Liguori L,Lukas J,Monti MC,Morretta E,Cubellis MV,Andreotti G

PA5-27349 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to confirm the necessity of personalized therapies for Fabry patients and paved the way to further studies and trials of treatments for Fabry disease.

Fri Jan 06 00:00:00 EST 2023

Drug Repositioning for Fabry Disease: Acetylsalicylic Acid Potentiates the Stabilization of Lysosomal Alpha-Galactosidase by Pharmacological Chaperones.

International journal of molecular sciences

Monticelli M,Liguori L,Allocca M,Bosso A,Andreotti G,Lukas J,Monti MC,Morretta E,Cubellis MV,Hay Mele B

Published figure using alpha Galactosidase polyclonal antibody (Product # PA5-27349) in Western Blot

Wed May 04 00:00:00 EDT 2022

The Relative Abundances of Human Leukocyte Antigen-E, α-Galactosidase A and α-Gal Antigenic Determinants Are Biased by Trichostatin A-Dependent Epigenetic Transformation of Triple-Transgenic Pig-Derived Dermal Fibroblast Cells.

International journal of molecular sciences

Samiec M,Wiater J,Wartalski K,Skrzyszowska M,Trzcińska M,Lipiński D,Jura J,Smorąg Z,Słomski R,Duda M

Published figure using alpha Galactosidase polyclonal antibody (Product # PA5-27349) in Immunocytochemistry

Wed Sep 07 00:00:00 EDT 2022

The Relative Abundances of Human Leukocyte Antigen-E, α-Galactosidase A and α-Gal Antigenic Determinants Are Biased by Trichostatin A-Dependent Epigenetic Transformation of Triple-Transgenic Pig-Derived Dermal Fibroblast Cells.

International journal of molecular sciences

Samiec M,Wiater J,Wartalski K,Skrzyszowska M,Trzcińska M,Lipiński D,Jura J,Smorąg Z,Słomski R,Duda M

Published figure using alpha Galactosidase polyclonal antibody (Product # PA5-27349) in Immunocytochemistry

Wed Sep 07 00:00:00 EDT 2022

Characterization of Mono- and Bi-Transgenic Pig-Derived Epidermal Keratinocytes Expressing Human FUT2 and GLA Genes-In Vitro Studies.

International journal of molecular sciences

Wiater J,Samiec M,Wartalski K,Smorąg Z,Jura J,Słomski R,Skrzyszowska M,Romek M

Published figure using alpha Galactosidase polyclonal antibody (Product # PA5-27349) in Western Blot

Tue Sep 07 00:00:00 EDT 2021

Trichostatin A-Assisted Epigenomic Modulation Affects the Expression Profiles of Not Only Recombinant Human α1,2-Fucosyltransferase and α-Galactosidase A Enzymes But Also Galα1→3Gal Epitopes in Porcine Bi-Transgenic Adult Cutaneous Fibroblast Cells.

International journal of molecular sciences

Wiater J,Samiec M,Skrzyszowska M,Lipiński D

PA5-27349 was used in Western Blot to explore whether trichostatin A-assisted epigenomic modulation (TSA-EM) can affect the expression of not only recombinant human α1,2-fucosyltransferase (rhα1,2-FT) and α-galactosidase A (rhα-Gal A) immune system enzymes but also Galα1→3Gal epitopes in ex vivo proliferating adult cutaneous fibroblast cells (ACFCs) derived from hFUT2×hGLA bi-transgenic pigs that had been produced for the needs of future xenotransplantatio

Sat Jan 30 00:00:00 EST 2021

Trichostatin A-Assisted Epigenomic Modulation Affects the Expression Profiles of Not Only Recombinant Human α1,2-Fucosyltransferase and α-Galactosidase A Enzymes But Also Galα1→3Gal Epitopes in Porcine Bi-Transgenic Adult Cutaneous Fibroblast Cells.

International journal of molecular sciences

Wiater J,Samiec M,Skrzyszowska M,Lipiński D

PA5-27349 was used in Western Blot to explore whether trichostatin A-assisted epigenomic modulation (TSA-EM) can affect the expression of not only recombinant human α1,2-fucosyltransferase (rhα1,2-FT) and α-galactosidase A (rhα-Gal A) immune system enzymes but also Galα1→3Gal epitopes in ex vivo proliferating adult cutaneous fibroblast cells (ACFCs) derived from hFUT2×hGLA bi-transgenic pigs that had been produced for the needs of future xenotransplantatio

Sat Jan 30 00:00:00 EST 2021

Bioinformatics

Protein Aliases: agalsidase alfa; Alpha-D-galactosidase A; Alpha-D-galactoside galactohydrolase; alpha-D-galactoside galactohydrolase 1; alpha-gal A; Alpha-galactosidase A; galactosidase, alpha; Galactosylgalactosylglucosylceramidase GLA; Melibiase

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Gene Aliases: GALA; GLA

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UniProt ID: (Human) P06280

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Entrez Gene ID: (Human) 2717

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catalytic activity alpha-galactosidase activity receptor binding protein binding hydrolase activity galactoside binding protein homodimerization activity raffinose alpha-galactosidase activity galactosidase

Process(es)

glycosphingolipid metabolic process oligosaccharide metabolic process negative regulation of nitric oxide biosynthetic process glycosylceramide catabolic process glycosphingolipid catabolic process negative regulation of nitric-oxide synthase activity

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