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Invitrogen
beta-5 Tubulin Recombinant Rabbit Monoclonal Antibody (SR25-04)
FIGURE: 1 / 12
beta-5 Tubulin Antibody (MA5-32008) in ICC/IF
Immunocytochemical analysis of beta-5 Tubulin in Hela cells using a beta-5 Tubulin Monoclonal antibody (Product # MA5-32008) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32008
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SR25-04
Immunogen
Synthetic peptide within Human beta Tubulin aa 308-357
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809302
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tublin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. This antibody does not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Ab-3 does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta Ib tubulin; tbb5; tubulin beta; Tubulin beta chain; tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, beta class I; tubulin, beta polypeptide
Gene Aliases: AA408537; AI596182; B130022C14Rik; CDCBM6; CSCSC1; M(beta)5; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB5
UniProt ID: (Human) P07437, (Mouse) P99024, (Rat) P69897
Entrez Gene ID: (Human) 203068, (Mouse) 22154, (Rat) 29214
G2/M transition of mitotic cell cycle
movement of cell or subcellular component
microtubule-based process
cellular process
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
spindle assembly
cell division
protein polymerization
biological_process
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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