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Invitrogen
beta Galactosidase Fusion Protein Polyclonal Antibody, eBioscience™
FIGURE: 1 / 2
beta Galactosidase Fusion Protein Antibody (14-6773-81) in IHC
PLoS genetics 2018 -
Fig 6 Hyperactivity of P53 signaling in the NCC- mTOR KO mice. (A) Western blots for Mdm2, P53, P21 and Bax of E10.5 and E11.5 facial primordia. (B) Quantification of western blots of Mdm2, P53, P21 and Bax. (C, D) Immunofluorescence for P53 in the mandibular prominence of E10.5 mice. (E, F) Immunofluorescence for beta-galactosidase in the mandibular arch of E14.5 mice. Note, a number of positive cells are ... View More
Product Details
14-6773-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Published species
Bacteria,
Fruit fly,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with BSA, 0.02% gelatin
Contains
0.1% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468331
Product Specific Information
Description: The rabbit polyclonal antibody reacts with bBeta galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope.
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.
Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Beta-Gal; betagal; bgal; ß-gal; ß-galactosidase; ßgal
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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