Invitrogen

beta Tubulin Polyclonal Antibody

beta Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta Tubulin Antibody (PA5-16863) in ICC/IF

Immunofluorescence analysis of Tubulin Beta was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Tubulin Beta Rabbit Polyclonal Antibody (Product # PA5-16863) at 2 µg/mL in 0.1% BSA and incubated for 3 ho... View More

beta Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta Tubulin Antibody in Western Blot (WB)

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Product Details

PA5-16863

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Plant, Rat, Sheep, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from aa 416-430 of human tubulin beta

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.09 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986058

Product Specific Information

PA5-16863 targets Tubulin Beta in IHC (P), WB, and IP applications and shows reactivity with Amphibian, Bovine, Chicken, Fungi, Gerbil, Guinea Pig, Human, mouse, Porcine, Rat, Sea Urchin, and Yeast samples.

The PA5-16863 immunogen is a synthetic peptide derived from aa 416-430 of human tubulin beta.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters.

International journal of molecular sciences

Merckx C,Cosemans G,Zschüntzsch J,Raedt R,Schmidt J,De Paepe B,De Bleecker JL

PA5-16863 was used in Western Blotting to highlight the potential of osmolyte pathway members as a research interest and future therapeutic target in dystrophinopathy.

Thu Mar 17 00:00:00 EDT 2022

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Molecular cell

Stoneley M,Harvey RF,Mulroney TE,Mordue R,Jukes-Jones R,Cain K,Lilley KS,Sawarkar R,Willis AE

PA5-16863 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that ribosome stalling activates cell-cycel arrest, which may be prolonged due to inadequate functioning of ASCC to resolve the stalled ribosomes.

Thu Apr 21 00:00:00 EDT 2022

Nanotopography modulates cytoskeletal organization and dynamics during T cell activation.

Molecular biology of the cell

Wheatley BA,Rey-Suarez I,Hourwitz MJ,Kerr S,Shroff H,Fourkas JT,Upadhyaya A

PA5-16863 was used in Immunocytochemistry-immunofluorescence to investigate how nanotopography modulates cytoskeletal dynamics and signaling during the early stages of T cell activation using high-resolution fluorescence microscopy on nanofabricated surfaces with parallel nanoridges of different spacings.

Thu Sep 01 00:00:00 EDT 2022

CTP synthase is essential for early endosperm development by regulating nuclei spacing.

Plant biotechnology journal

Yoon J,Cho LH,Kim SR,Tun W,Peng X,Pasriga R,Moon S,Hong WJ,Ji H,Jung KH,Jeon JS,An G

PA5-16863 was used in Immunohistochemistry to identify the rice mutant endospermless 2 which shows defects in the early stages of endosperm development.

Mon Nov 01 00:00:00 EDT 2021

Cigarette Smoke Activates NOTCH3 to Promote Goblet Cell Differentiation in Human Airway Epithelial Cells.

American journal of respiratory cell and molecular biology

Bodas M,Moore AR,Subramaniyan B,Georgescu C,Wren JD,Freeman WM,Brown BR,Metcalf JP,Walters MS

PA5-16863 was used in Western Blot to identify NOTCH3 activation as one of the important mechanisms by which cigarette smoke induces goblet cell differentiation, thus providing a novel potential strategy to control GCMH-related pathologies in smokers and patients with COPD.

Thu Apr 01 00:00:00 EDT 2021

A mouse model of Huntington's disease shows altered ultrastructure of transverse tubules in skeletal muscle fibers.

The Journal of general physiology

Romer SH,Metzger S,Peraza K,Wright MC,Jobe DS,Song LS,Rich MM,Foy BD,Talmadge RJ,Voss AA

PA5-16863 was used in Western Blot to explain dysregulated EC coupling and motor impairment in Huntington's disease.

Mon Apr 05 00:00:00 EDT 2021

A mouse model of Huntington's disease shows altered ultrastructure of transverse tubules in skeletal muscle fibers.

The Journal of general physiology

Romer SH,Metzger S,Peraza K,Wright MC,Jobe DS,Song LS,Rich MM,Foy BD,Talmadge RJ,Voss AA

PA5-16863 was used in Western Blot to explain dysregulated EC coupling and motor impairment in Huntington's disease.

Mon Apr 05 00:00:00 EDT 2021

Prognostic value of SH3PXD2B (Tks4) in human hepatocellular carcinoma: a combined multi-omics and experimental study.

BMC medical genomics

Kui X,Wang Y,Zhang C,Li H,Li Q,Ke Y,Wang L

Published figure using beta Tubulin polyclonal antibody (Product # PA5-16863) in Western Blot

Wed Apr 28 00:00:00 EDT 2021

Prognostic value of SH3PXD2B (Tks4) in human hepatocellular carcinoma: a combined multi-omics and experimental study.

BMC medical genomics

Kui X,Wang Y,Zhang C,Li H,Li Q,Ke Y,Wang L

Published figure using beta Tubulin polyclonal antibody (Product # PA5-16863) in Western Blot

Wed Apr 28 00:00:00 EDT 2021

The Gastroprotective Effect of Naringenin against Ethanol-Induced Gastric Ulcers in Mice through Inhibiting Oxidative and Inflammatory Responses.

International journal of molecular sciences

Li WS,Lin SC,Chu CH,Chang YK,Zhang X,Lin CC,Tung YT

PA5-16863 was used in Western Blot to show that pretreatment with naringenin significantly protected mice from ethanol-induced hemorrhagic damage, epithelial cell loss, and edema with leucocytes.

Fri Nov 05 00:00:00 EDT 2021

The Gastroprotective Effect of Naringenin against Ethanol-Induced Gastric Ulcers in Mice through Inhibiting Oxidative and Inflammatory Responses.

International journal of molecular sciences

Li WS,Lin SC,Chu CH,Chang YK,Zhang X,Lin CC,Tung YT

PA5-16863 was used in Western Blot to show that pretreatment with naringenin significantly protected mice from ethanol-induced hemorrhagic damage, epithelial cell loss, and edema with leucocytes.

Fri Nov 05 00:00:00 EDT 2021

eIF5A-Independent Role of DHPS in p21^CIP1 and Cell Fate Regulation.

International journal of molecular sciences

Becker AE,Wu PK,Park JI

PA5-16863 was used in Western Blotting to demonstrate that DHPS has a role independent of eIF5A hypusination in A375 and SK-MEL-28 human melanoma cells, in which the extracellular signal regulated kinase 1/2 (ERK1/2) pathway is deregulated.

Tue Dec 07 00:00:00 EST 2021

Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica.

International journal of molecular medicine

Yang C,Yang Y,Ma L,Zhang GX,Shi FD,Yan Y,Chang G

PA5-16863 was used in Western Blotting to compare the proliferation, differentiation and senescence of bone marrow-derived mesenchymal stem cells from patients with Neuromyelitis optica with that of age- and sex-matched healthy subjects.

Fri Mar 01 00:00:00 EST 2019

Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against Pseudomonas aeruginosa lung infection.

The Journal of biological chemistry

Lillehoj EP,Guang W,Hyun SW,Liu A,Hegerle N,Simon R,Cross AS,Ishida H,Luzina IG,Atamas SP,Goldblum SE

PA5-16863 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate MUC1-ED desialylation in lung infection.

Fri Jan 11 00:00:00 EST 2019

Ribosome-bound Pub1 modulates stop codon decoding during translation termination in yeast.

The FEBS journal

Urakov VN,Mitkevich OV,Safenkova IV,Ter-Avanesyan MD

PA5-16863 was used in Western Blotting to study the role of yeast protein, Pub1, in translation termination.

Thu Jun 01 00:00:00 EDT 2017

Aged blood factors decrease cellular responses associated with delayed gingival wound repair.

PloS one

Saldías MP,Fernández C,Morgan A,Díaz C,Morales D,Jaña F,Gómez A,Silva A,Briceño F,Oyarzún A,Maldonado F,Cerda O,Smith PC,Cáceres M

PA5-16863 was used in Western Blotting to examine the in vivo wound healing process using a gingival repair rat model and an in vitro model using human gingival fibroblast for cellular responses associated to wound healing.

Wed Oct 18 00:00:00 EDT 2017

Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis.

Human molecular genetics

Cottle DL,Ursino GM,Ip SC,Jones LK,Ditommaso T,Hacking DF,Mangan NE,Mellett NA,Henley KJ,Sviridov D,Nold-Petry CA,Nold MF,Meikle PJ,Kile BT,Smyth IM

PA5-16863 was used in western blot to examine the developing epidermis to determine the consequences of lipid dysregulation in mouse models.

Thu Jan 15 00:00:00 EST 2015

Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2+ in Hansenula polymorpha.

PloS one

Fokina AV,Chechenova MB,Karginov AV,Ter-Avanesyan MD,Agaphonov MO

PA5-16863 was used in western blot to study calcium deficiency in the secretory pathway caused by Pmr1 inactivation

Mon Jun 27 00:00:00 EDT 2016

Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.

Biology open

Asano S,Nemoto T,Kitayama T,Harada K,Zhang J,Harada K,Tanida I,Hirata M,Kanematsu T

PA5-16863 was used in immunocytochemistry to study the role of phospholipase C-related catalytically inactive protein in KIF5B-mediated insulin secretion

Thu May 08 00:00:00 EDT 2014

N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation.

Biochemical and biophysical research communications

Ishimine H,Yamakawa N,Sasao M,Tadokoro M,Kami D,Komazaki S,Tokuhara M,Takada H,Ito Y,Kuno S,Yoshimura K,Umezawa A,Ohgushi H,Asashima M,Kurisaki A

PA5-16863 was used in western blot to study the value of N-cadherin expression as a prospective marker of human mesenchymal stem cells that have a high probability of differentiating into cardiomyocytes

Fri Sep 06 00:00:00 EDT 2013

Chronic pulsatile hyperglycemia reduces insulin secretion and increases accumulation of reactive oxygen species in fetal sheep islets.

The Journal of endocrinology

Green AS,Chen X,Macko AR,Anderson MJ,Kelly AC,Hart NJ,Lynch RM,Limesand SW

PA5-16863 was used in western blot to study insulin secretion and ROS accumulation in pancreatic islets from fetal sheep subjected to chronic pulsed hyperglycemia

Thu Mar 01 00:00:00 EST 2012

Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia.

The Journal of endocrinology

Batista ML,Neves RX,Peres SB,Yamashita AS,Shida CS,Farmer SR,Seelaender M

PA5-16863 was used in western blot to study cancer cachexia and the temporal response of different adipose tissue depots

Sat Dec 01 00:00:00 EST 2012

A subset of human gliomas shows over-expression of KIT without its amplification.

Gene

Saini M,Jha AN,Abrari A,Ali S

PA5-16863 was used in western blot to study the overexpression of KIT in the absence of gene amplification as observed in a subset of human glioma patients

Sun Apr 15 00:00:00 EDT 2012

Expression of proto-oncogene KIT is up-regulated in subset of human meningiomas.

BMC cancer

Saini M,Jha AN,Abrari A,Ali S

PA5-16863 was used in western blot to study the upregulation of the proto-oncogene KIT in a subset of human meningiomas

Wed Jun 06 00:00:00 EDT 2012

Neurite outgrowth of mature retinal ganglion cells and PC12 cells requires activity of CK1δ and CK1ε.

PloS one

Bischof J,Müller A,Fänder M,Knippschild U,Fischer D

PA5-16863 was used in immunocytochemistry to determine the role of CK1delta and CK1epsilon activity in neurite outgrowth of lens injury stimulated retinal ganglion cells and nerve growth factor stimulated PC12 cells

Mon Nov 07 00:00:00 EST 2011

Bioinformatics

Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5

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Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding ubiquitin protein ligase binding protein complex binding GTPase activating protein binding MHC class I protein binding peptide binding molecular_function nucleotide binding tubulin

Process(es)

G2/M transition of mitotic cell cycle movement of cell or subcellular component microtubule-based process cellular process cytoskeleton-dependent intracellular transport natural killer cell mediated cytotoxicity spindle assembly cell division cytoskeleton organization neuron migration mitotic nuclear division axon guidance biological_process protein polymerization metabolic process neuron differentiation cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta Tubulin Polyclonal Antibody

beta Tubulin Antibody (PA5-16863) in ICC/IF

Product Details

PA5-16863

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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