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extracellular ASIC1 Antibody (OSA00064W-100UL) in IHC (P)
IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Pr... View More
Product Details
OSA00064W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 100-150 of the extracellular domain of rat ASIC1 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in mouse and human.
Specificity of this antibody: ASIC1.
Target Information
This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACCN2 variant 3; acid sensing (proton gated) ion channel 1; acid sensing ion channel; acid sensing ion channel 1; acid-sensing (proton-gated) ion channel 1; Acid-sensing ion channel; Acid-sensing ion channel 1; acid-sensing ion channel 1a protein; Amiloride-sensitive cation channel 2, neuronal; ASIC1; BNaC2; Brain sodium channel 2; Cation channel, amiloride-sensitive, neuronal, 2; degenerin 2; hBNaC2; proton gated cation channel ASIC1
Gene Aliases: ACCN2; AI843610; ASIC; ASIC1; ASIC1a; B530003N02Rik; BNAC2
UniProt ID: (Human) P78348, (Mouse) Q6NXK8, (Rat) P55926
Entrez Gene ID: (Human) 41, (Mouse) 11419, (Rat) 79123
transport
sodium ion transport
signal transduction
memory
associative learning
response to pH
response to acidic pH
ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
negative regulation of neurotransmitter secretion
sensory perception of sour taste
protein homotrimerization
calcium ion transmembrane transport
cellular response to pH
ion transport
cation transport
calcium ion transport
monovalent inorganic cation transport
sensory perception of pain
cation transmembrane transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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