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gamma tubulin Monoclonal Antibody (3F9H8)

gamma tubulin Antibody in Immunocytochemistry (ICC/IF)

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gamma tubulin Antibody (66320-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (Product # 66320-1-Ig) (Clone: 3F9H8) at dilution of 1:1,000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

gamma tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

gamma tubulin Antibody in Western Blot (WB)

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Product Details

66320-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 14 publications 14 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3F9H8

Immunogen

gamma tubulin Fusion Protein Ag23913 (150-451 aa encoded by BC000619)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can label the centrosome structure.

Immunogen sequence: GSYLLERLN DRYPKKLVQT YSVFPNQDEM SDVVVQPYNS LLTLKRLTQN ADCVVVLDNT ALNRIATDRL HIQNPSFSQI NQLVSTIMSA STTTLRYPGY MNNDLIGLIA SLIPTPRLHF LMTGYTPLTT DQSVASVRKT TVLDVMRRLL QPKNVMVSTG RDRQTNHCYI AILNIIQGEV DPTQVHKSLQ RIRERKLANF IPWGPASIQV ALSRKSPYLP SAHRVSGLMM ANHTSISSLF ERTCRQYDKL RKREAFLEQF RKEDMFKDNF DEMDTSREIV QQLIDEYHAA TRPDYISWGT QEQ (150-451 aa encoded by BC000619)

Target Information

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation.

Cell death & disease

Song C,Zhang Y,Li Y,Bie J,Wang Z,Yang X,Li H,Zhu L,Zhang T,Chang Q,Luo J

Thu Feb 09 00:00:00 EST 2023

The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation.

Cell death & disease

Song C,Zhang Y,Li Y,Bie J,Wang Z,Yang X,Li H,Zhu L,Zhang T,Chang Q,Luo J

Thu Feb 09 00:00:00 EST 2023

Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids.

Stem cells international

Ning K,Luo Z,Kowal TJ,Tran M,Majumder R,Jarin TM,Wu AY,Goldberg JL,Sun Y

Wed Feb 22 00:00:00 EST 2023

Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4.

iScience

Zhang C,Ma X,Wei G,Zhu X,Hu P,Chen X,Wang D,Li Y,Ruan T,Zhang W,Tao K,Wu C

Fri Jan 20 00:00:00 EST 2023

Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4.

iScience

Zhang C,Ma X,Wei G,Zhu X,Hu P,Chen X,Wang D,Li Y,Ruan T,Zhang W,Tao K,Wu C

Fri Jan 20 00:00:00 EST 2023

MYO10 regulates genome stability and cancer inflammation through mediating mitosis.

Cell reports

Mayca Pozo F,Geng X,Miyagi M,Amin AL,Huang AY,Zhang Y

Tue May 30 00:00:00 EDT 2023

Inhibition of Ciliogenesis Enhances the Cellular Sensitivity to Temozolomide and Ionizing Radiation in Human Glioblastoma Cells.

Biomedical and environmental sciences : BES

Wei L,Ma W,Cai H,Peng SP,Tian HB,Wang JF,Gao L,He JP

Fri May 20 00:00:00 EDT 2022

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca²⁺ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Journal of nanobiotechnology

Su L,Sun Z,Qi F,Su H,Qian L,Li J,Zuo L,Huang J,Yu Z,Li J,Chen Z,Zhang S

Wed Jul 20 00:00:00 EDT 2022

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca²⁺ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Journal of nanobiotechnology

Su L,Sun Z,Qi F,Su H,Qian L,Li J,Zuo L,Huang J,Yu Z,Li J,Chen Z,Zhang S

Wed Jul 20 00:00:00 EDT 2022

CEP192 is a novel prognostic marker and correlates with the immune microenvironment in hepatocellular carcinoma.

Frontiers in immunology

Liu Y,Liang W,Chang Y,He Z,Wu M,Zheng H,Ke X,Lv M,Liu Q,Liu Q,Tang W,Huang Q,Lu Y,He M,Yang Q,Mo C,Wang J,Peng K,Min Z,Su H,Chen J

Mon Oct 17 00:00:00 EDT 2022

The phytochemical, corynoline, diminishes Aurora kinase B activity to induce mitotic defect and polyploidy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yan Z,Shi Q,Liu X,Li J,Ahire V,Zhang S,Zhang J,Yang D,Allen TD

Tue Mar 01 00:00:00 EST 2022

Modulation of Primary Cilia by Alvocidib Inhibition of CILK1.

International journal of molecular sciences

Wang EX,Turner JS,Brautigan DL,Fu Z

Sat Jul 23 00:00:00 EDT 2022

Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells.

The Journal of comparative neurology

Kowal TJ,Dhande OS,Wang B,Wang Q,Ning K,Liu W,Berbari NF,Hu Y,Sun Y

Mon Aug 01 00:00:00 EDT 2022

Time-resolved proteomics profiling of the ciliary Hedgehog response.

The Journal of cell biology

May EA,Kalocsay M,D'Auriac IG,Schuster PS,Gygi SP,Nachury MV,Mick DU

Mon May 03 00:00:00 EDT 2021

Polycaprolactone Electrospun Scaffolds Produce an Enrichment of Lung Cancer Stem Cells in Sensitive and Resistant EGFRm Lung Adenocarcinoma.

Cancers

Polonio-Alcalá E,Rabionet M,Ruiz-Martínez S,Palomeras S,Porta R,Vásquez-Dongo C,Bosch-Barrera J,Puig T,Ciurana J

Fri Oct 22 00:00:00 EDT 2021

The Phytochemical Scoulerine Inhibits Aurora Kinase Activity to Induce Mitotic and Cytokinetic Defects.

Journal of natural products

Li J,Yan Z,Li H,Shi Q,Ahire V,Zhang S,Nimishetti N,Yang D,Allen TD,Zhang J

Fri Aug 27 00:00:00 EDT 2021

Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling.

EMBO molecular medicine

Bosakova M,Abraham SP,Nita A,Hruba E,Buchtova M,Taylor SP,Duran I,Martin J,Svozilova K,Barta T,Varecha M,Balek L,Kohoutek J,Radaszkiewicz T,Pusapati GV,Bryja V,Rush ET,Thiffault I,Nickerson DA,Bamshad MJ,Rohatgi R,Cohn DH,Krakow D,Krejci P

Fri Nov 06 00:00:00 EST 2020

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Nature

Deniston CK,Salogiannis J,Mathea S,Snead DM,Lahiri I,Matyszewski M,Donosa O,Watanabe R,Böhning J,Shiau AK,Knapp S,Villa E,Reck-Peterson SL,Leschziner AE

Tue Dec 01 00:00:00 EST 2020

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.

eLife

Hu M,Schulze KE,Ghildyal R,Henstridge DC,Kolanowski JL,New EJ,Hong Y,Hsu AC,Hansbro PM,Wark PA,Bogoyevitch MA,Jans DA

Thu Jun 27 00:00:00 EDT 2019

Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells.

Oncotarget

Ma LL,Shen L,Tong GH,Tang N,Luo Y,Guo LL,Hu CT,Huang YX,Huang G,Jing FY,Liu C,Li ZY,Zhou N,Yan QW,Lei Y,Zhu SJ,Cheng ZQ,Cao GW,Deng YJ,Ding YQ

Fri Sep 29 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: centrosome marker; gamma tubulin; Gamma-1-tubulin; Gamma-2-tubulin; Gamma-tubulin complex component 1; GCP-1; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide

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Gene Aliases: 1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; GCP-1; TUBG; TUBG1; TUBG2; TUBGCP1; Tubgl

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UniProt ID: (Human) P23258, (Dog) Q9GKK5, (Human) Q9NRH3, (Mouse) P83887, (Rat) P83888, (Mouse) Q8VCK3

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Entrez Gene ID: (Human) 7283, (Dog) 403695, (Human) 27175, (Mouse) 103733, (Rat) 252921, (Rat) 680991, (Mouse) 103768

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Function(s)

GTPase activity structural constituent of cytoskeleton protein binding GTP binding structural molecule activity nucleotide binding tubulin

Process(es)

G2/M transition of mitotic cell cycle meiotic spindle organization microtubule cytoskeleton organization microtubule nucleation cytoplasmic microtubule organization microtubule-based process mitotic spindle organization regulation of mitotic nuclear division protein polymerization metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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gamma tubulin Monoclonal Antibody (3F9H8)

gamma tubulin Antibody (66320-1-IG) in ICC/IF

Product Details

66320-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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