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p47phox Antibody (MA5-42546) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of p47-phox in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with p47phox Monoclonal Antibody (Product # MA5-42546) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain i... View More
Product Details
MA5-42546
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A6B11
Immunogen
Recombinant protein within human p47-phox aa 51-250
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911687
Product Specific Information
Positive Control: Raji cell lysates, Daudi cell lysates, Hela, NIH/3T3, human lung tissue, human spleen tissue, Daudi.
Subcellular Location: Cytosol, Membrane.
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NADPH oxidase subunit (47 kD); NADPH oxidase subunit (47kDa); NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; SH3 and PX domain-containing protein 1A
Gene Aliases:
Ncf-1; NCF-47K; NCF1; NCF1A; NOXO2; p47-phox; p47
UniProt ID: (Human) P14598, (Mouse) Q09014
Entrez Gene ID: (Human) 653361, (Mouse) 17969
response to yeast
leukocyte mediated cytotoxicity
respiratory burst involved in defense response
protein targeting to membrane
leukotriene metabolic process
NADP catabolic process
apoptotic process
inflammatory response
cellular defense response
cell proliferation
response to bacterium
superoxide anion generation
defense response to bacterium
respiratory burst
negative regulation of smooth muscle contraction
hydrogen peroxide biosynthetic process
defense response to Gram-positive bacterium
defense response to fungus
oxidation-reduction process
neutrophil mediated killing of gram-positive bacterium
neutrophil mediated killing of fungus
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
superoxide metabolic process
positive regulation of catalytic activity
innate immune response
cell redox homeostasis
vascular endothelial growth factor receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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