Gibco

Human CCL19 (MIP-3 beta) Recombinant Protein, PeproTech®

Product Details

300-29B-20UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 43 publications 43 publications

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse, Zebrafish

Expression System

E. coli

Amino acid sequence

GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS

Molecular weight

8.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human T cells using a concentration of 10.0-50.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

300-29B-1MG will be provided as 2 x 500 µg (300-29B-500UG).

Recombinant Human MIP-3beta is an 8.8 kDa protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma.

Discover. Oncology

Zhou WH,Wang Y,Yan C,Du WD,Al-Aroomi MA,Zheng L,Lin SF,Gao JX,Jiang S,Wang ZX,Sun CF,Liu FY

300-29B was used in In vitro experiments to investigate CCR7 in tumour associated macrophage recruitment of oral squamous cell carcinoma.

Fri Jul 29 00:00:00 EDT 2022

G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators.

Nature communications

Gurusamy M,Tischner D,Shao J,Klatt S,Zukunft S,Bonnavion R,Günther S,Siebenbrodt K,Kestner RI,Kuhlmann T,Fleming I,Offermanns S,Wettschureck N

Tue Nov 23 00:00:00 EST 2021

CAL-1 as Cellular Model System to Study CCR7-Guided Human Dendritic Cell Migration.

Frontiers in immunology

Uetz-von Allmen E,Samson GPB,Purvanov V,Maeda T,Legler DF

300-29B was used in In vitro experiments to show that the previously established human neoplastic cell line CAL-1 expresses the human dendritic cell surface antigens CD11c and HLA-DR together with co-stimulatory molecules.

Mon Dec 13 00:00:00 EST 2021

Selective cell death of latently HIV-infected CD4⁺ T cells mediated by autosis inducing nanopeptides.

Cell death & disease

Zhang G,Luk BT,Wei X,Campbell GR,Fang RH,Zhang L,Spector SA

Wed May 29 00:00:00 EDT 2019

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

A tissue-like platform for studying engineered quiescent human T-cells' interactions with dendritic cells.

eLife

Abu-Shah E,Demetriou P,Bálint Š,Mayya V,Kutuzov MA,Dushek O,Dustin ML

300-29B was used in Sample Preparation to report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell and characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity.

Wed Sep 25 00:00:00 EDT 2019

SMAC Mimetics Induce Autophagy-Dependent Apoptosis of HIV-1-Infected Resting Memory CD4+ T Cells.

Cell host & microbe

Campbell GR,Bruckman RS,Chu YL,Trout RN,Spector SA

300-29B was used in Cell Culture to study how DIABLO/SMAC mimetics (birinapant, GDC-0152, and embelin) selectively kill HIV-Long-lived resting memory CD4+ T cells (TCM) without increasing virus production or targeting uninfected TCM.

Wed Nov 14 00:00:00 EST 2018

Specific oxylipins enhance vertebrate hematopoiesis via the receptor GPR132.

Proceedings of the National Academy of Sciences of the United States of America

Lahvic JL,Ammerman M,Li P,Blair MC,Stillman ER,Fast EM,Robertson AL,Christodoulou C,Perlin JR,Yang S,Chiang N,Norris PC,Daily ML,Redfield SE,Chan IT,Chatrizeh M,Chase ME,Weis O,Zhou Y,Serhan CN,Zon LI

Tue Sep 11 00:00:00 EDT 2018

Specific oxylipins enhance vertebrate hematopoiesis via the receptor GPR132.

Proceedings of the National Academy of Sciences of the United States of America

Tue Sep 11 00:00:00 EDT 2018

Lymphatic exosomes promote dendritic cell migration along guidance cues.

The Journal of cell biology

Brown M,Johnson LA,Leone DA,Majek P,Vaahtomeri K,Senfter D,Bukosza N,Schachner H,Asfour G,Langer B,Hauschild R,Parapatics K,Hong YK,Bennett KL,Kain R,Detmar M,Sixt M,Jackson DG,Kerjaschki D

Mon Jun 04 00:00:00 EDT 2018

Compromised functionality of monocyte-derived dendritic cells in multiple myeloma patients may limit their use in cancer immunotherapy.

Scientific reports

Shinde P,Fernandes S,Melinkeri S,Kale V,Limaye L

Mon Apr 09 00:00:00 EDT 2018

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

300-29B was used in Sample Preparation to demonstrate that glucokinase-dependent glycolysis regulates regulatory T cell migration.

Tue Nov 21 00:00:00 EST 2017

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

300-29B was used in Sample Preparation to demonstrate that glucokinase-dependent glycolysis regulates regulatory T cell migration.

Tue Nov 21 00:00:00 EST 2017

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Functional Langerinhigh-Expressing Langerhans-like Cells Can Arise from CD14highCD16- Human Blood Monocytes in Serum-Free Condition.

Journal of immunology (Baltimore, Md. : 1950)

Picarda G,Chéneau C,Humbert JM,Bériou G,Pilet P,Martin J,Duteille F,Perrot P,Bellier-Waast F,Heslan M,Haspot F,Guillon F,Josien R,Halary FA

Sun May 01 00:00:00 EDT 2016

The Mammalian Sterile 20-like 1 Kinase Controls Selective CCR7-Dependent Functions in Human Dendritic Cells.

Journal of immunology (Baltimore, Md. : 1950)

Torres-Bacete J,Delgado-Martín C,Gómez-Moreira C,Simizu S,Rodríguez-Fernández JL

Sat Aug 01 00:00:00 EDT 2015

Galectin-1 regulates tissue exit of specific dendritic cell populations.

The Journal of biological chemistry

Thiemann S,Man JH,Chang MH,Lee B,Baum LG

Fri Sep 11 00:00:00 EDT 2015

KIR2DS1-dependent acquisition of CCR7 and migratory properties by human NK cells interacting with allogeneic HLA-C2+ DCs or T-cell blasts.

Blood

Marcenaro E,Pesce S,Sivori S,Carlomagno S,Moretta L,Moretta A

Thu Apr 25 00:00:00 EDT 2013

Chronic lymphocytic leukemia cells induce defective LFA-1-directed T-cell motility by altering Rho GTPase signaling that is reversible with lenalidomide.

Blood

Ramsay AG,Evans R,Kiaii S,Svensson L,Hogg N,Gribben JG

Thu Apr 04 00:00:00 EDT 2013

β-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR.

The Journal of biological chemistry

Watts AO,Verkaar F,van der Lee MM,Timmerman CA,Kuijer M,van Offenbeek J,van Lith LH,Smit MJ,Leurs R,Zaman GJ,Vischer HF

Fri Mar 08 00:00:00 EST 2013

Leukotriene C4 induces migration of human monocyte-derived dendritic cells without loss of immunostimulatory function.

Blood

Dannull J,Schneider T,Lee WT,de Rosa N,Tyler DS,Pruitt SK

Thu Mar 29 00:00:00 EDT 2012

L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Freeley M,O'Dowd F,Paul T,Kashanin D,Davies A,Kelleher D,Long A

Fri Jun 15 00:00:00 EDT 2012

Adenosine deaminase enhances the immunogenicity of human dendritic cells from healthy and HIV-infected individuals.

PloS one

Casanova V,Naval-Macabuhay I,Massanella M,Rodríguez-García M,Blanco J,Gatell JM,García F,Gallart T,Lluis C,Mallol J,Franco R,Climent N,McCormick PJ

Thu May 30 00:00:00 EDT 2013

CD81 is essential for the formation of membrane protrusions and regulates Rac1-activation in adhesion-dependent immune cell migration.

Blood

Quast T,Eppler F,Semmling V,Schild C,Homsi Y,Levy S,Lang T,Kurts C,Kolanus W

Thu Aug 18 00:00:00 EDT 2011

Mycolactone impairs T cell homing by suppressing microRNA control of L-selectin expression.

Proceedings of the National Academy of Sciences of the United States of America

Guenin-Macé L,Carrette F,Asperti-Boursin F,Le Bon A,Caleechurn L,Di Bartolo V,Fontanet A,Bismuth G,Demangel C

Tue Aug 02 00:00:00 EDT 2011

Cross-talk between TCR and CCR7 signaling sets a temporal threshold for enhanced T lymphocyte migration.

Journal of immunology (Baltimore, Md. : 1950)

Schaeuble K,Hauser MA,Singer E,Groettrup M,Legler DF

Thu Dec 01 00:00:00 EST 2011

Dendritic cells exposed to MVA-based HIV-1 vaccine induce highly functional HIV-1-specific CD8(+) T cell responses in HIV-1-infected individuals.

PloS one

Climent N,Guerra S,García F,Rovira C,Miralles L,Gómez CE,Piqué N,Gil C,Gatell JM,Esteban M,Gallart T

Mon Nov 28 00:00:00 EST 2011

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

Chemokine CXCL12 uses CXCR4 and a signaling core formed by bifunctional Akt, extracellular signal-regulated kinase (ERK)1/2, and mammalian target of rapamycin complex 1 (mTORC1) proteins to control chemotaxis and survival simultaneously in mature dendritic cells.

The Journal of biological chemistry

Delgado-Martín C,Escribano C,Pablos JL,Riol-Blanco L,Rodríguez-Fernández JL

Fri Oct 28 00:00:00 EDT 2011

Uncarinic Acid C Isolated from Uncaria rhynchophylla Induces Differentiation of Th1-Promoting Dendritic Cells Through TLR4 Signaling.

Biomarker insights

Kim KS,Pham TN,Jin CJ,Umeyama A,Shoji N,Hashimoto T,Lee JJ,Takei M

Mon Feb 28 00:00:00 EST 2011

PGE(2) transiently enhances DC expression of CCR7 but inhibits the ability of DCs to produce CCL19 and attract naive T cells.

Blood

Muthuswamy R,Mueller-Berghaus J,Haberkorn U,Reinhart TA,Schadendorf D,Kalinski P

Thu Sep 02 00:00:00 EDT 2010

Cyclin-dependent kinase 5 activity is required for T cell activation and induction of experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Pareek TK,Lam E,Zheng X,Askew D,Kulkarni AB,Chance MR,Huang AY,Cooke KR,Letterio JJ

Mon Oct 25 00:00:00 EDT 2010

Chemokine scavenger D6 is expressed by trophoblasts and aids the survival of mouse embryos transferred into allogeneic recipients.

Journal of immunology (Baltimore, Md. : 1950)

Madigan J,Freeman DJ,Menzies F,Forrow S,Nelson SM,Young A,Sharkey A,Moffett A,Graham GJ,Greer IA,Rot A,Nibbs RJ

Mon Mar 15 00:00:00 EDT 2010

CCR7 signalling as an essential regulator of CNS infiltration in T-cell leukaemia.

Nature

Buonamici S,Trimarchi T,Ruocco MG,Reavie L,Cathelin S,Mar BG,Klinakis A,Lukyanov Y,Tseng JC,Sen F,Gehrie E,Li M,Newcomb E,Zavadil J,Meruelo D,Lipp M,Ibrahim S,Efstratiadis A,Zagzag D,Bromberg JS,Dustin ML,Aifantis I

Thu Jun 18 00:00:00 EDT 2009

CCR7 signalling as an essential regulator of CNS infiltration in T-cell leukaemia.

Nature

Thu Jun 18 00:00:00 EDT 2009

An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.

Blood

Trinidad EM,Ballesteros M,Zuloaga J,Zapata A,Alonso-Colmenar LM

Thu Dec 03 00:00:00 EST 2009

CCL21 mediates CD4+ T-cell costimulation via a DOCK2/Rac-dependent pathway.

Blood

Gollmer K,Asperti-Boursin F,Tanaka Y,Okkenhaug K,Vanhaesebroeck B,Peterson JR,Fukui Y,Donnadieu E,Stein JV

Thu Jul 16 00:00:00 EDT 2009

Chemokine receptor expression and functional effects of chemokines on B cells: implication in the pathogenesis of rheumatoid arthritis.

Arthritis research & therapy

Nanki T,Takada K,Komano Y,Morio T,Kanegane H,Nakajima A,Lipsky PE,Miyasaka N

Wed Feb 03 00:00:00 EST 2010

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 EDT 2009

Cysteinyl-leukotriene receptor type 1 expression and function is down-regulated during monocyte-derived dendritic cell maturation with zymosan: involvement of IL-10 and prostaglandins.

Journal of immunology (Baltimore, Md. : 1950)

Thivierge M,Stankova J,Rola-Pleszczynski M

Sun Nov 15 00:00:00 EST 2009

Human cytomegalovirus paralyzes macrophage motility through down-regulation of chemokine receptors, reorganization of the cytoskeleton, and release of macrophage migration inhibitory factor.

Journal of immunology (Baltimore, Md. : 1950)

Frascaroli G,Varani S,Blankenhorn N,Pretsch R,Bacher M,Leng L,Bucala R,Landini MP,Mertens T

Thu Jan 01 00:00:00 EST 2009

Anthrax edema toxin induces maturation of dendritic cells and enhances chemotaxis towards macrophage inflammatory protein 3beta.

Infection and immunity

Maldonado-Arocho FJ,Bradley KA

Fri May 01 00:00:00 EDT 2009

Bone marrow-derived dendritic cells generated in the presence of resolvin E1 induce apoptosis of activated CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Vassiliou EK,Kesler OM,Tadros JH,Ganea D

Wed Oct 01 00:00:00 EDT 2008

Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Lee J,Hillsamer P,Kim CH

Tue Jan 01 00:00:00 EST 2008

Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors.

Stem cells (Dayton, Ohio)

Honczarenko M,Le Y,Swierkowski M,Ghiran I,Glodek AM,Silberstein LE

Sat Apr 01 00:00:00 EST 2006

CC-chemokine ligand 16 induces a novel maturation program in human immature monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cappello P,Fraone T,Barberis L,Costa C,Hirsch E,Elia AR,Caorsi C,Musso T,Novelli F,Giovarelli M

Wed Nov 01 00:00:00 EST 2006

Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Broxmeyer HE,Kim CH

Sat Jul 15 00:00:00 EDT 2006

Role of ChemR23 in directing the migration of myeloid and plasmacytoid dendritic cells to lymphoid organs and inflamed skin.

The Journal of experimental medicine

Vermi W,Riboldi E,Wittamer V,Gentili F,Luini W,Marrelli S,Vecchi A,Franssen JD,Communi D,Massardi L,Sironi M,Mantovani A,Parmentier M,Facchetti F,Sozzani S

Mon Feb 21 00:00:00 EST 2005

Human herpesvirus 7 open reading frames U12 and U51 encode functional beta-chemokine receptors.

Journal of virology

Tadagaki K,Nakano K,Yamanishi K

Wed Jun 01 00:00:00 EDT 2005

Chemokine receptor-mediated delivery directs self-tumor antigen efficiently into the class II processing pathway in vitro and induces protective immunity in vivo.

Blood

Biragyn A,Ruffini PA,Coscia M,Harvey LK,Neelapu SS,Baskar S,Wang JM,Kwak LW

Fri Oct 01 00:00:00 EDT 2004

CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.

Blood

Honczarenko M,Le Y,Glodek AM,Majka M,Campbell JJ,Ratajczak MZ,Silberstein LE

Tue Oct 01 00:00:00 EDT 2002

CCR11 is a functional receptor for the monocyte chemoattractant protein family of chemokines.

The Journal of biological chemistry

Schweickart VL,Epp A,Raport CJ,Gray PW

Fri Mar 31 00:00:00 EST 2000

Bioinformatics

Protein Aliases: beta chemokine exodus-3; Beta-chemokine exodus-3; C-C motif chemokine; C-C motif chemokine 19; CC chemokine ligand 19; chemokine (C-C motif) ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; M-MIP-3-beta; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MIP-3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

Human CCL19 (MIP-3 beta) Recombinant Protein, PeproTech®

Product Details

300-29B-20UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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