Gibco

Human CCL2 (MCP-1), Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-300-04-20UG

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human

Expression System

E. coli

Amino acid sequence

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Molecular weight

8.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant human MCP-1 is an 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3.

Scientific reports

He H,Kuriyan AE,Su CW,Mahabole M,Zhang Y,Zhu YT,Flynn HW,Parel JM,Tseng SC

AF-300-04 was used in In vitro experiments to investigate heavy chain-hyaluronic acid/pentraxin 3 inhibition of proliferative vitreoretinopathy.

Thu Mar 02 00:00:00 EST 2017

BDNF Pretreatment of Human Embryonic-Derived Neural Stem Cells Improves Cell Survival and Functional Recovery After Transplantation in Hypoxic-Ischemic Stroke.

Cell transplantation

Rosenblum S,Smith TN,Wang N,Chua JY,Westbroek E,Wang K,Guzman R

AF-300-04 was used in In vitro experiments to evaluate if pretreatment of NSCs with brain-derived neurotrophic factor (BDNF) prior to transplantation improves cell engraftment and functional recovery following hypoxic-ischemic (HI) stroke.

Wed Oct 19 00:00:00 EDT 2016

Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.

Journal of immunology (Baltimore, Md. : 1950)

Cross SA,Cook DR,Chi AW,Vance PJ,Kolson LL,Wong BJ,Jordan-Sciutto KL,Kolson DL

Tue Nov 15 00:00:00 EST 2011

Chemokine (C-C motif) ligand 2 mediates direct and indirect fibrotic responses in human and murine cultured fibrocytes.

Fibrogenesis & tissue repair

Ekert JE,Murray LA,Das AM,Sheng H,Giles-Komar J,Rycyzyn MA

Wed Oct 19 00:00:00 EDT 2011

From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay.

PloS one

Kraemer S,Vaught JD,Bock C,Gold L,Katilius E,Keeney TR,Kim N,Saccomano NA,Wilcox SK,Zichi D,Sanders GM

Fri Feb 17 00:00:00 EST 2012

Microparticles from apoptotic platelets promote resident macrophage differentiation.

Cell death & disease

Vasina EM,Cauwenberghs S,Feijge MA,Heemskerk JW,Weber C,Koenen RR

Thu Sep 29 00:00:00 EDT 2011

The first subcomponent of complement, C1q, triggers the production of IL-8, IL-6, and monocyte chemoattractant peptide-1 by human umbilical vein endothelial cells.

Journal of immunology (Baltimore, Md. : 1950)

van den Berg RH,Faber-Krol MC,Sim RB,Daha MR

Tue Dec 15 00:00:00 EST 1998

The first subcomponent of complement, C1q, triggers the production of IL-8, IL-6, and monocyte chemoattractant peptide-1 by human umbilical vein endothelial cells.

Journal of immunology (Baltimore, Md. : 1950)

van den Berg RH,Faber-Krol MC,Sim RB,Daha MR

Tue Dec 15 00:00:00 EST 1998

The first subcomponent of complement, C1q, triggers the production of IL-8, IL-6, and monocyte chemoattractant peptide-1 by human umbilical vein endothelial cells.

Journal of immunology (Baltimore, Md. : 1950)

van den Berg RH,Faber-Krol MC,Sim RB,Daha MR

Tue Dec 15 00:00:00 EST 1998

Type I IFNs enhance the terminal differentiation of dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Luft T,Pang KC,Thomas E,Hertzog P,Hart DN,Trapani J,Cebon J

Sat Aug 15 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

Human CCL2 (MCP-1), Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-300-04-20UG

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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