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Product Details
A42570
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD38, amino acids Val43-Ile300 (Accession # NP_001766.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
31.3 kDa
Class
Recombinant
Type
Protein
Purity
>97 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide
(NGD+) to cyclic GDPribose.The specific activity is > 2, 500 pmoles/min/µg.
2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.
3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind Human CD38 His-tag Recombinant Protein with an affinity constant of 5.429 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
(NGD+) to cyclic GDPribose.The specific activity is > 2, 500 pmoles/min/µg.
2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.
3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind Human CD38 His-tag Recombinant Protein with an affinity constant of 5.429 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Target Information
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10
Gene Aliases: ADPRC 1; ADPRC1; CD38
UniProt ID: (Human) P28907
Entrez Gene ID: (Human) 952
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