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Human GDNF, Animal-Free Recombinant Protein, PeproTech®

Human GDNF, Animal-Free Protein in Functional Assay (Functional)

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Human GDNF, Animal-Free Protein (AF-450-10-10UG) in Functional

Bioassay analysis of Human GDNF, Animal-Free Recombinant Protein, PeproTech® (Product # AF-450-10-1MG).

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Product Details

AF-450-10-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Non-human primate

Expression System

E. coli

Amino acid sequence

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Molecular weight

30.4 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Programmable RNA targeting with the single-protein CRISPR effector Cas7-11.

Nature

Özcan A,Krajeski R,Ioannidi E,Lee B,Gardner A,Makarova KS,Koonin EV,Abudayyeh OO,Gootenberg JS

Wed Sep 01 00:00:00 EDT 2021

Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs.

Cell death & disease

Brighi C,Salaris F,Soloperto A,Cordella F,Ghirga S,de Turris V,Rosito M,Porceddu PF,D'Antoni C,Reggiani A,Rosa A,Di Angelantonio S

Sat May 15 00:00:00 EDT 2021

Generation and validation of APOE knockout human iPSC-derived cerebral organoids.

STAR protocols

Martens YA,Xu S,Tait R,Li G,Zhao XC,Lu W,Liu CC,Kanekiyo T,Bu G,Zhao J

AF-450-10 was used in Cell Culture to describe a protocol for efficient knockout of apolipoprotein E in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system.

Fri Jun 18 00:00:00 EDT 2021

Doublecortin-like Kinase 1 Regulates α-Synuclein Levels and Toxicity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Vázquez-Vélez GE,Gonzales KA,Revelli JP,Adamski CJ,Alavi Naini F,Bajić A,Craigen E,Richman R,Heman-Ackah SM,Wood MJA,Rousseaux MWC,Zoghbi HY

AF-450-10 was used in Cell Culture to provide evidence for a novel function for DCLK1 in the mature brain, and for its potential as a new therapeutic target for synucleinopathies.

Wed Jan 08 00:00:00 EST 2020

Pharmacological enhancement of TFEB-mediated autophagy alleviated neuronal death in oxidative stress-induced Parkinson's disease models.

Cell death & disease

Zhuang XX,Wang SF,Tan Y,Song JX,Zhu Z,Wang ZY,Wu MY,Cai CZ,Huang ZJ,Tan JQ,Su HX,Li M,Lu JH

Tue Feb 18 00:00:00 EST 2020

Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling.

Cell death & disease

Ke M,Chong CM,Zeng H,Huang M,Huang Z,Zhang K,Cen X,Lu JH,Yao X,Qin D,Su H

Tue Feb 18 00:00:00 EST 2020

JMJD3 and UTX determine fidelity and lineage specification of human neural progenitor cells.

Nature communications

Shan Y,Zhang Y,Zhao Y,Wang T,Zhang J,Yao J,Ma N,Liang Z,Huang W,Huang K,Zhang T,Su Z,Chen Q,Zhu Y,Wu C,Zhou T,Sun W,Wei Y,Zhang C,Li C,Su S,Liao B,Zhong M,Zhong X,Nie J,Pei D,Pan G

Mon Jan 20 00:00:00 EST 2020

CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual.

Communications biology

Yun Y,Hong SA,Kim KK,Baek D,Lee D,Londhe AM,Lee M,Yu J,McEachin ZT,Bassell GJ,Bowser R,Hales CM,Cho SR,Kim J,Pae AN,Cheong E,Kim S,Boulis NM,Bae S,Ha Y

Mon Jan 20 00:00:00 EST 2020

Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning.

Nature neuroscience

Sellgren CM,Gracias J,Watmuff B,Biag JD,Thanos JM,Whittredge PB,Fu T,Worringer K,Brown HE,Wang J,Kaykas A,Karmacharya R,Goold CP,Sheridan SD,Perlis RH

AF-450-10 was used in Cell Culture to demonstrate increased synapse elimination in patient-derived neural cultures and isolated synaptosomes.

Fri Mar 01 00:00:00 EST 2019

mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome.

Scientific reports

Crutcher E,Pal R,Naini F,Zhang P,Laugsch M,Kim J,Bajic A,Schaaf CP

Mon Nov 04 00:00:00 EST 2019

Intrinsic Immunity Shapes Viral Resistance of Stem Cells.

Cell

Wu X,Dao Thi VL,Huang Y,Billerbeck E,Saha D,Hoffmann HH,Wang Y,Silva LAV,Sarbanes S,Sun T,Andrus L,Yu Y,Quirk C,Li M,MacDonald MR,Schneider WM,An X,Rosenberg BR,Rice CM

Thu Jan 25 00:00:00 EST 2018

ATXN1-CIC Complex Is the Primary Driver of Cerebellar Pathology in Spinocerebellar Ataxia Type 1 through a Gain-of-Function Mechanism.

Neuron

Rousseaux MWC,Tschumperlin T,Lu HC,Lackey EP,Bondar VV,Wan YW,Tan Q,Adamski CJ,Friedrich J,Twaroski K,Chen W,Tolar J,Henzler C,Sharma A,Bajić A,Lin T,Duvick L,Liu Z,Sillitoe RV,Zoghbi HY,Orr HT

Wed Mar 21 00:00:00 EDT 2018

The transition from stem cell to progenitor spermatogonia and male fertility requires the SHP2 protein tyrosine phosphatase.

Stem cells (Dayton, Ohio)

Puri P,Phillips BT,Suzuki H,Orwig KE,Rajkovic A,Lapinski PE,King PD,Feng GS,Walker WH

Sat Mar 01 00:00:00 EST 2014

Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.

Cell

Xue Y,Ouyang K,Huang J,Zhou Y,Ouyang H,Li H,Wang G,Wu Q,Wei C,Bi Y,Jiang L,Cai Z,Sun H,Zhang K,Zhang Y,Chen J,Fu XD

Thu Jan 17 00:00:00 EST 2013

Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.

Cell

Xue Y,Ouyang K,Huang J,Zhou Y,Ouyang H,Li H,Wang G,Wu Q,Wei C,Bi Y,Jiang L,Cai Z,Sun H,Zhang K,Zhang Y,Chen J,Fu XD

Thu Jan 17 00:00:00 EST 2013

Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques.

The Journal of clinical investigation

Hayashi T,Wakao S,Kitada M,Ose T,Watabe H,Kuroda Y,Mitsunaga K,Matsuse D,Shigemoto T,Ito A,Ikeda H,Fukuyama H,Onoe H,Tabata Y,Dezawa M

Tue Jan 01 00:00:00 EST 2013

Increased proteasome activity in human embryonic stem cells is regulated by PSMD11.

Nature

Vilchez D,Boyer L,Morantte I,Lutz M,Merkwirth C,Joyce D,Spencer B,Page L,Masliah E,Berggren WT,Gage FH,Dillin A

Thu Sep 13 00:00:00 EDT 2012

Increasing doublecortin expression promotes migration of human embryonic stem cell-derived neurons.

Stem cells (Dayton, Ohio)

Filipovic R,Santhosh Kumar S,Fiondella C,Loturco J

Sat Sep 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Astrocyte-derived trophic factor; ATF; Glial cell line-derived neurotrophic factor; H-GDNF; hGDNF

Human GDNF, Animal-Free Recombinant Protein, PeproTech®

Human GDNF, Animal-Free Protein (AF-450-10-10UG) in Functional

Product Details

AF-450-10-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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