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Invitrogen
Human IFN-alpha 2 (alpha 2b) Recombinant Protein
Product Details
111051
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Molecular weight
19.3 kDa
Class
Recombinant
Type
Protein
Purity
>95%
Endotoxin concentration
<1 EU/µg
Activity
EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alpha-A) provided by the National Institutes of Health [see Pestka, S. (1986). Methods in Enzymology, Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).
Conjugate
Form
Liquid
Concentration
Lot-specific
Purification
purified
Storage buffer
PBS with 0.1% BSA
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
After receipt, the product may be stored at -20°C for short-term use (less than 6 months). For long-term storage, store the product at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum.
Target Information
Interferon alpha-2b is a cytokine that belongs to the type I interferon family and is encoded by a gene cluster on chromosome 9. It is produced in response to viral infection and plays a key role in the innate immune response, exhibiting potent antiviral, antiproliferative and immunomodulatory properties. Interferon alpha-2b binds to a plasma membrane receptor consisting of IFNAR1 and IFNAR2, which is expressed ubiquitously in the body and can thus act on virtually all cells. This cytokine is effective in reducing the symptoms and duration of the common cold, and has shown efficacy in treating various types of cancer, including hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is considered a hallmark of severe COVID-19, and a combination therapy involving interferon alpha-2b may be a potential therapeutic approach. IFNA2 has been associated with diseases such as Severe Covid-19 and Hepatitis B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-2a interferon; IFN-alpha-2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; Interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A
Gene Aliases: IFN-alphaA; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; INFA2
UniProt ID: (Human) P01563
Entrez Gene ID: (Human) 3440
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