Gibco

Human IL-3, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-200-03-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 43 publications 43 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Molecular weight

15 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel variants in Krueppel like factor 1 that cause persistence of fetal hemoglobin in In(Lu) individuals.

Scientific reports

Eernstman J,Veldhuisen B,Ligthart P,von Lindern M,van der Schoot CE,van den Akker E

Fri Sep 17 00:00:00 EDT 2021

NTAL is associated with treatment outcome, cell proliferation and differentiation in acute promyelocytic leukemia.

Scientific reports

Thomé CH,Ferreira GA,Pereira-Martins DA,Dos Santos GA,Ortiz CA,de Souza LEB,Sobral LM,Silva CLA,Scheucher PS,Gil CD,Leopoldino AM,Silveira DRA,Coelho-Silva JL,Traina F,Koury LC,Melo RAM,Bittencourt R,Pagnano K,Pasquini R,Nunes EC,Fagundes EM,Gloria ABF,Kerbauy FR,Chauffaille ML,Keating A,Tallman MS,Ribeiro RC,Dillon R,Ganser A,Löwenberg B,Valk P,Lo-Coco F,Sanz MA,Berliner N,Faça VM,Rego EM

Thu Jun 25 00:00:00 EDT 2020

Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype.

Nature communications

Thornton N,Karamatic Crew V,Tilley L,Green CA,Tay CL,Griffiths RE,Singleton BK,Spring F,Walser P,Alattar AG,Jones B,Laundy R,Storry JR,Möller M,Wall L,Charlewood R,Westhoff CM,Lomas-Francis C,Yahalom V,Feick U,Seltsam A,Mayer B,Olsson ML,Anstee DJ

Thu Jul 16 00:00:00 EDT 2020

Human iPSC-derived mature microglia retain their identity and functionally integrate in the chimeric mouse brain.

Nature communications

Xu R,Li X,Boreland AJ,Posyton A,Kwan K,Hart RP,Jiang P

Fri Mar 27 00:00:00 EDT 2020

Neutrophils Derived from Genetically Modified Human Induced Pluripotent Stem Cells Circulate and Phagocytose Bacteria In Vivo.

Stem cells translational medicine

Trump LR,Nayak RC,Singh AK,Emberesh S,Wellendorf AM,Lutzko CM,Cancelas JA

Sat Jun 01 00:00:00 EDT 2019

Gene correction for SCID-X1 in long-term hematopoietic stem cells.

Nature communications

Pavel-Dinu M,Wiebking V,Dejene BT,Srifa W,Mantri S,Nicolas CE,Lee C,Bao G,Kildebeck EJ,Punjya N,Sindhu C,Inlay MA,Saxena N,DeRavin SS,Malech H,Roncarolo MG,Weinberg KI,Porteus MH

AF-200-03 was used in In vitro experiments to evaluate the use of SCID-X1 in targeting the cDNA in human long-term hematopoietic stem cells.

Tue Apr 09 00:00:00 EDT 2019

Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells.

Scientific reports

Song L,Yuan X,Jones Z,Vied C,Miao Y,Marzano M,Hua T,Sang QA,Guan J,Ma T,Zhou Y,Li Y

Tue Jul 30 00:00:00 EDT 2019

TAF1 plays a critical role in AML1-ETO driven leukemogenesis.

Nature communications

Xu Y,Man N,Karl D,Martinez C,Liu F,Sun J,Martinez CJ,Martin GM,Beckedorff F,Lai F,Yue J,Roisman A,Greenblatt S,Duffort S,Wang L,Sun X,Figueroa M,Shiekhattar R,Nimer S

Tue Oct 29 00:00:00 EDT 2019

Regulatory NLRs Control the RLR-Mediated Type I Interferon and Inflammatory Responses in Human Dendritic Cells.

Frontiers in immunology

Fekete T,Bencze D,Szabo A,Csoma E,Biro T,Bacsi A,Pazmandi K

AF-200-03 was used in Cell Culture to exhibit that NLRX1 is the primary regulator of RLR-mediated innate immune response in human dendritic cells, while NLRC5s only hold a supplemntary role.

Thu Oct 03 00:00:00 EDT 2019

Gene therapy for Wiskott-Aldrich syndrome in a severely affected adult.

Blood

Morris EC,Fox T,Chakraverty R,Tendeiro R,Snell K,Rivat C,Grace S,Gilmour K,Workman S,Buckland K,Butler K,Chee R,Salama AD,Ibrahim H,Hara H,Duret C,Mavilio F,Male F,Bushman FD,Galy A,Burns SO,Gaspar HB,Thrasher AJ

Thu Sep 14 00:00:00 EDT 2017

Engraftment and in vivo proliferation advantage of gene-corrected mobilized CD34⁺ cells from Fanconi anemia patients.

Blood

Río P,Navarro S,Guenechea G,Sánchez-Domínguez R,Lamana ML,Yañez R,Casado JA,Mehta PA,Pujol MR,Surrallés J,Charrier S,Galy A,Segovia JC,Díaz de Heredia C,Sevilla J,Bueren JA

Thu Sep 28 00:00:00 EDT 2017

Enhanced Generation of Integration-free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors.

Stem cell reports

Wen W,Zhang JP,Xu J,Su RJ,Neises A,Ji GZ,Yuan W,Cheng T,Zhang XB

Tue Jun 14 00:00:00 EDT 2016

Distinct immunological activation profiles of dSLIM® and ProMune® depend on their different structural context.

Immunity, inflammation and disease

Kapp K,Schneider J,Schneider L,Gollinge N,Jänsch S,Schroff M,Wittig B,Kleuss C

Thu Dec 01 00:00:00 EST 2016

Role of SOX17 in hematopoietic development from human embryonic stem cells.

Blood

Nakajima-Takagi Y,Osawa M,Oshima M,Takagi H,Miyagi S,Endoh M,Endo TA,Takayama N,Eto K,Toyoda T,Koseki H,Nakauchi H,Iwama A

Thu Jan 17 00:00:00 EST 2013

Genomic impact of transient low-dose decitabine treatment on primary AML cells.

Blood

Klco JM,Spencer DH,Lamprecht TL,Sarkaria SM,Wylie T,Magrini V,Hundal J,Walker J,Varghese N,Erdmann-Gilmore P,Lichti CF,Meyer MR,Townsend RR,Wilson RK,Mardis ER,Ley TJ

Thu Feb 28 00:00:00 EST 2013

Preclinical safety and efficacy of human CD34(+) cells transduced with lentiviral vector for the treatment of Wiskott-Aldrich syndrome.

Molecular therapy : the journal of the American Society of Gene Therapy

Scaramuzza S,Biasco L,Ripamonti A,Castiello MC,Loperfido M,Draghici E,Hernandez RJ,Benedicenti F,Radrizzani M,Salomoni M,Ranzani M,Bartholomae CC,Vicenzi E,Finocchi A,Bredius R,Bosticardo M,Schmidt M,von Kalle C,Montini E,Biffi A,Roncarolo MG,Naldini L,Villa A,Aiuti A

Tue Jan 01 00:00:00 EST 2013

TNF-α has tropic rather than apoptotic activity in human hematopoietic progenitors: involvement of TNF receptor-1 and caspase-8.

Stem cells (Dayton, Ohio)

Mizrahi K,Stein J,Yaniv I,Kaplan O,Askenasy N

Tue Jan 01 00:00:00 EST 2013

The two-component adjuvant IC31® boosts type i interferon production of human monocyte-derived dendritic cells via ligation of endosomal TLRs.

PloS one

Szabo A,Gogolak P,Pazmandi K,Kis-Toth K,Riedl K,Wizel B,Lingnau K,Bacsi A,Rethi B,Rajnavolgyi E

Mon Dec 02 00:00:00 EST 2013

Fluvastatin inhibits FLT3 glycosylation in human and murine cells and prolongs survival of mice with FLT3/ITD leukemia.

Blood

Williams AB,Li L,Nguyen B,Brown P,Levis M,Small D

Thu Oct 11 00:00:00 EDT 2012

Fluvastatin inhibits FLT3 glycosylation in human and murine cells and prolongs survival of mice with FLT3/ITD leukemia.

Blood

Williams AB,Li L,Nguyen B,Brown P,Levis M,Small D

Thu Oct 11 00:00:00 EDT 2012

Stem and progenitor cells in myelodysplastic syndromes show aberrant stage-specific expansion and harbor genetic and epigenetic alterations.

Blood

Will B,Zhou L,Vogler TO,Ben-Neriah S,Schinke C,Tamari R,Yu Y,Bhagat TD,Bhattacharyya S,Barreyro L,Heuck C,Mo Y,Parekh S,McMahon C,Pellagatti A,Boultwood J,Montagna C,Silverman L,Maciejewski J,Greally JM,Ye BH,List AF,Steidl C,Steidl U,Verma A

Thu Sep 06 00:00:00 EDT 2012

Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells.

Blood

Gori JL,Chandrasekaran D,Kowalski JP,Adair JE,Beard BC,D'Souza SL,Kiem HP

Thu Sep 27 00:00:00 EDT 2012

Characterization of novel genomic alterations and therapeutic approaches using acute megakaryoblastic leukemia xenograft models.

The Journal of experimental medicine

Thiollier C,Lopez CK,Gerby B,Ignacimouttou C,Poglio S,Duffourd Y,Guégan J,Rivera-Munoz P,Bluteau O,Mabialah V,Diop M,Wen Q,Petit A,Bauchet AL,Reinhardt D,Bornhauser B,Gautheret D,Lecluse Y,Landman-Parker J,Radford I,Vainchenker W,Dastugue N,de Botton S,Dessen P,Bourquin JP,Crispino JD,Ballerini P,Bernard OA,Pflumio F,Mercher T

Mon Oct 22 00:00:00 EDT 2012

Perturbation of fetal liver hematopoietic stem and progenitor cell development by trisomy 21.

Proceedings of the National Academy of Sciences of the United States of America

Roy A,Cowan G,Mead AJ,Filippi S,Bohn G,Chaidos A,Tunstall O,Chan JK,Choolani M,Bennett P,Kumar S,Atkinson D,Wyatt-Ashmead J,Hu M,Stumpf MP,Goudevenou K,O'Connor D,Chou ST,Weiss MJ,Karadimitris A,Jacobsen SE,Vyas P,Roberts I

Tue Oct 23 00:00:00 EDT 2012

Secretory leukocyte proteinase inhibitor-competent DNA deposits are potent stimulators of plasmacytoid dendritic cells: implication for psoriasis.

Journal of immunology (Baltimore, Md. : 1950)

Skrzeczynska-Moncznik J,Wlodarczyk A,Zabieglo K,Kapinska-Mrowiecka M,Marewicz E,Dubin A,Potempa J,Cichy J

Wed Aug 15 00:00:00 EDT 2012

TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-α-mediated antitumor reactivity.

Journal of immunology (Baltimore, Md. : 1950)

Kalb ML,Glaser A,Stary G,Koszik F,Stingl G

Wed Feb 15 00:00:00 EST 2012

Human umbilical cord blood-derived CD34+ cells reverse osteoporosis in NOD/SCID mice by altering osteoblastic and osteoclastic activities.

PloS one

Aggarwal R,Lu J,Kanji S,Joseph M,Das M,Noble GJ,McMichael BK,Agarwal S,Hart RT,Sun Z,Lee BS,Rosol TJ,Jackson R,Mao HQ,Pompili VJ,Das H

Wed Dec 12 00:00:00 EST 2012

GATA2 is a critical transactivator for the human IL1RL1/ST2 promoter in mast cells/basophils: opposing roles for GATA2 and GATA1 in human IL1RL1/ST2 gene expression.

The Journal of biological chemistry

Baba Y,Maeda K,Yashiro T,Inage E,Kasakura K,Suzuki R,Niyonsaba F,Hara M,Tanabe A,Ogawa H,Okumura K,Ohtsuka Y,Shimizu T,Nishiyama C

Fri Sep 21 00:00:00 EDT 2012

Residual expression of the reprogramming factors prevents differentiation of iPSC generated from human fibroblasts and cord blood CD34+ progenitors.

PloS one

Ramos-Mejía V,Montes R,Bueno C,Ayllón V,Real PJ,Rodríguez R,Menendez P

Mon Aug 27 00:00:00 EDT 2012

Acetylation of histone deacetylase 1 regulates NuRD corepressor complex activity.

The Journal of biological chemistry

Yang T,Jian W,Luo Y,Fu X,Noguchi C,Bungert J,Huang S,Qiu Y

Fri Nov 23 00:00:00 EST 2012

Direct chromatin PCR (DC-PCR): hypotonic conditions allow differentiation of chromatin states during thermal cycling.

PloS one

Vatolin S,Khan SN,Reu FJ

Tue Mar 12 00:00:00 EDT 2013

Suppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells.

The Journal of biological chemistry

Gao H,Wu X,Sun Y,Zhou S,Silberstein LE,Zhu Z

Fri Aug 24 00:00:00 EDT 2012

Micro-RNA30c negatively regulates REDD1 expression in human hematopoietic and osteoblast cells after gamma-irradiation.

PloS one

Li XH,Ha CT,Fu D,Xiao M

Thu May 09 00:00:00 EDT 2013

Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications.

PloS one

Yamagata Y,Parietti V,Stockholm D,Corre G,Poinsignon C,Touleimat N,Delafoy D,Besse C,Tost J,Galy A,Paldi A

Wed Jul 24 00:00:00 EDT 2013

HBsAg inhibits IFN-α production in plasmacytoid dendritic cells through TNF-α and IL-10 induction in monocytes.

PloS one

Shi B,Ren G,Hu Y,Wang S,Zhang Z,Yuan Z

Fri Feb 15 00:00:00 EST 2013

Activation of STAT6 by STING is critical for antiviral innate immunity.

Cell

Chen H,Sun H,You F,Sun W,Zhou X,Chen L,Yang J,Wang Y,Tang H,Guan Y,Xia W,Gu J,Ishikawa H,Gutman D,Barber G,Qin Z,Jiang Z

Fri Oct 14 00:00:00 EDT 2011

Targeting the SH2-kinase interface in Bcr-Abl inhibits leukemogenesis.

Cell

Grebien F,Hantschel O,Wojcik J,Kaupe I,Kovacic B,Wyrzucki AM,Gish GD,Cerny-Reiterer S,Koide A,Beug H,Pawson T,Valent P,Koide S,Superti-Furga G

Fri Oct 14 00:00:00 EDT 2011

Rho kinase regulates the survival and transformation of cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL.

Cancer cell

Mali RS,Ramdas B,Ma P,Shi J,Munugalavadla V,Sims E,Wei L,Vemula S,Nabinger SC,Goodwin CB,Chan RJ,Traina F,Visconte V,Tiu RV,Lewis TA,Stern AM,Wen Q,Crispino JD,Boswell HS,Kapur R

Tue Sep 13 00:00:00 EDT 2011

BCR-ABL suppresses autophagy through ATF5-mediated regulation of mTOR transcription.

Blood

Sheng Z,Ma L,Sun JE,Zhu LJ,Green MR

Thu Sep 08 00:00:00 EDT 2011

Production of the plasma-cell survival factor a proliferation-inducing ligand (APRIL) peaks in myeloid precursor cells from human bone marrow.

Blood

Matthes T,Dunand-Sauthier I,Santiago-Raber ML,Krause KH,Donze O,Passweg J,McKee T,Huard B

Thu Aug 18 00:00:00 EDT 2011

The histone methyltransferase inhibitor, DZNep, up-regulates TXNIP, increases ROS production, and targets leukemia cells in AML.

Blood

Zhou J,Bi C,Cheong LL,Mahara S,Liu SC,Tay KG,Koh TL,Yu Q,Chng WJ

Thu Sep 08 00:00:00 EDT 2011

Glycogen synthase kinase-3β is a prosurvival signal for the maintenance of human mast cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Rådinger M,Smrž D,Metcalfe DD,Gilfillan AM

Thu Dec 01 00:00:00 EST 2011

Specific cellular signal-transduction responses to in vivo combination therapy with ATRA, valproic acid and theophylline in acute myeloid leukemia.

Blood cancer journal

Skavland J,Jørgensen KM,Hadziavdic K,Hovland R,Jonassen I,Bruserud O,Gjertsen BT

Tue Feb 01 00:00:00 EST 2011

Amino-terminal enhancer of split (AES) interacts with the oncoprotein NUP98-HOXA9 and enhances its transforming ability.

The Journal of biological chemistry

Sarma NJ,Yaseen NR

Fri Nov 11 00:00:00 EST 2011

Congenital dyserythropoietic anemia type II: molecular analysis and expression of the SEC23B gene.

Orphanet journal of rare diseases

Punzo F,Bertoli-Avella AM,Scianguetta S,Della Ragione F,Casale M,Ronzoni L,Cappellini MD,Forni G,Oostra BA,Perrotta S

Fri Dec 30 00:00:00 EST 2011

Bioinformatics

Protein Aliases: BP/BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA

Human IL-3, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-200-03-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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