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Human IL-7, Animal-Free Recombinant Protein, PeproTech®

Human IL-7, Animal-Free Protein in Functional Assay (Functional)

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Human IL-7, Animal-Free Protein (AF-200-07-10UG) in Functional

WHO Comparison of Human IL-7, Animal-Free Recombinant Protein, PeproTech® (Product # AF-200-07-1MG).

Product Image Gallery

Product Details

AF-200-07-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 33 publications 33 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Hamster, Human, Mouse, Rabbit, Virus

Expression System

E. coli

Amino acid sequence

MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH

Molecular weight

17.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of mouse 2E8 cells. The expected ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-7 is a 17.5 kDa protein containing 153 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A T cell redirection platform for co-targeting dual antigens on solid tumors.

mAbs

Enderle L,Shalaby KH,Gorelik M,Weiss A,Blazer LL,Paduch M,Cardarelli L,Kossiakoff A,Adams JJ,Sidhu SS

AF-200-07 was used in Cell Culture to engineer the Dual Antigen T cell Engager to enable T cell activity to specific solid cancer cell features.

Tue Dec 28 00:00:00 EST 2021

A biomimetic assay platform for the interrogation of antigen-dependent anti-tumor T-cell function.

Communications biology

To J,Quackenbush D,Rowell E,Li L,Reed C,Lo F,Horman SR

Fri Jan 08 00:00:00 EST 2021

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells.

Nature communications

Kluesner MG,Lahr WS,Lonetree CL,Smeester BA,Qiu X,Slipek NJ,Claudio Vázquez PN,Pitzen SP,Pomeroy EJ,Vignes MJ,Lee SC,Bingea SP,Andrew AA,Webber BR,Moriarity BS

Fri Apr 23 00:00:00 EDT 2021

Oncolytic measles virus therapy enhances tumor antigen-specific T-cell responses in patients with multiple myeloma.

Leukemia

Packiriswamy N,Upreti D,Zhou Y,Khan R,Miller A,Diaz RM,Rooney CM,Dispenzieri A,Peng KW,Russell SJ

Tue Dec 01 00:00:00 EST 2020

Distinct prognostic value of circulating anti-telomerase CD4⁺ Th1 immunity and exhausted PD-1⁺/TIM-3⁺ T cells in lung cancer.

British journal of cancer

Laheurte C,Dosset M,Vernerey D,Boullerot L,Gaugler B,Gravelin E,Kaulek V,Jacquin M,Cuche L,Eberst G,Jacoulet P,Fabre E,Le Pimpec-Barthes F,Tartour E,De Carvalho Bittencourt M,Westeel V,Adotévi O

Thu Aug 01 00:00:00 EDT 2019

In vitro Homeostatic Proliferation of Human CD8 T Cells.

Bio-protocol

Abdelsamed HA,Zebley CC,Youngblood B

AF-200-07 was used in Cell Culture to provide a protocol for the assessment of epigenetic programs in divided and undivided human naïve and memory CD8 T cells following 7 days in culture with IL-7 and IL-15 to illustrate how this approach can shed light on the mechanism(s) that governs the preservation of effector functions during homeostasis of long-lived memory CD8 T cells.

Mon Nov 20 00:00:00 EST 2017

Follistatin-like protein 1 contributes to dendritic cell and T-lymphocyte activation in nasopharyngeal carcinoma patients by altering nuclear factor κb and Jun N-terminal kinase expression.

Cell biochemistry and function

Wang H,Wu S,Huang S,Yin S,Zou G,Huang K,Zhang Z,Tang A,Wen W

Thu Dec 01 00:00:00 EST 2016

IL-7 and IL-15 instruct the generation of human memory stem T cells from naive precursors.

Blood

Cieri N,Camisa B,Cocchiarella F,Forcato M,Oliveira G,Provasi E,Bondanza A,Bordignon C,Peccatori J,Ciceri F,Lupo-Stanghellini MT,Mavilio F,Mondino A,Bicciato S,Recchia A,Bonini C

Thu Jan 24 00:00:00 EST 2013

Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.

PLoS pathogens

Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L

Fri Feb 01 00:00:00 EST 2013

Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.

PLoS pathogens

Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L

Fri Feb 01 00:00:00 EST 2013

IRX-2, a novel immunotherapeutic, enhances functions of human dendritic cells.

PloS one

Schilling B,Harasymczuk M,Schuler P,Egan J,Ferrone S,Whiteside TL

Fri Sep 06 00:00:00 EDT 2013

Functional heterogeneity of human effector CD8+ T cells.

Blood

Takata H,Naruto T,Takiguchi M

Thu Feb 09 00:00:00 EST 2012

Mast cells play a protumorigenic role in primary cutaneous lymphoma.

Blood

Rabenhorst A,Schlaak M,Heukamp LC,Förster A,Theurich S,von Bergwelt-Baildon M,Büttner R,Kurschat P,Mauch C,Roers A,Hartmann K

Thu Sep 06 00:00:00 EDT 2012

IL-7 induces expression and activation of integrin α4β7 promoting naive T-cell homing to the intestinal mucosa.

Blood

Cimbro R,Vassena L,Arthos J,Cicala C,Kehrl JH,Park C,Sereti I,Lederman MM,Fauci AS,Lusso P

Thu Sep 27 00:00:00 EDT 2012

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

Thu Dec 13 00:00:00 EST 2012

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

Thu Dec 13 00:00:00 EST 2012

Zebrafish screen identifies novel compound with selective toxicity against leukemia.

Blood

Ridges S,Heaton WL,Joshi D,Choi H,Eiring A,Batchelor L,Choudhry P,Manos EJ,Sofla H,Sanati A,Welborn S,Agarwal A,Spangrude GJ,Miles RR,Cox JE,Frazer JK,Deininger M,Balan K,Sigman M,Müschen M,Perova T,Johnson R,Montpellier B,Guidos CJ,Jones DA,Trede NS

Thu Jun 14 00:00:00 EDT 2012

Vav3 collaborates with p190-BCR-ABL in lymphoid progenitor leukemogenesis, proliferation, and survival.

Blood

Chang KH,Sanchez-Aguilera A,Shen S,Sengupta A,Madhu MN,Ficker AM,Dunn SK,Kuenzi AM,Arnett JL,Santho RA,Agirre X,Perentesis JP,Deininger MW,Zheng Y,Bustelo XR,Williams DA,Cancelas JA

Thu Jul 26 00:00:00 EDT 2012

Human T-lymphoid progenitors generated in a feeder-cell-free Delta-like-4 culture system promote T-cell reconstitution in NOD/SCID/γc(-/-) mice.

Stem cells (Dayton, Ohio)

Reimann C,Six E,Dal-Cortivo L,Schiavo A,Appourchaux K,Lagresle-Peyrou C,de Chappedelaine C,Ternaux B,Coulombel L,Beldjord K,Cavazzana-Calvo M,Andre-Schmutz I

Wed Aug 01 00:00:00 EDT 2012

Fetal-specific CD8+ cytotoxic T cell responses develop during normal human pregnancy and exhibit broad functional capacity.

Journal of immunology (Baltimore, Md. : 1950)

Lissauer D,Piper K,Goodyear O,Kilby MD,Moss PA

Sun Jul 15 00:00:00 EDT 2012

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

Thu Nov 01 00:00:00 EDT 2012

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

Thu Nov 01 00:00:00 EDT 2012

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

Thu Nov 01 00:00:00 EDT 2012

IL-2 upregulates CD86 expression on human CD4(+) and CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Paine A,Kirchner H,Immenschuh S,Oelke M,Blasczyk R,Eiz-Vesper B

Wed Feb 15 00:00:00 EST 2012

The IL-7Rα pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Kreft KL,Verbraak E,Wierenga-Wolf AF,van Meurs M,Oostra BA,Laman JD,Hintzen RQ

Wed Feb 15 00:00:00 EST 2012

Evidence for involvement of Th17 type responses in post kala azar dermal leishmaniasis (PKDL).

PLoS neglected tropical diseases

Katara GK,Ansari NA,Singh A,Ramesh V,Salotra P

Fri Sep 28 00:00:00 EDT 2012

Functional Differences between Human NKp44(-) and NKp44(+) RORC(+) Innate Lymphoid Cells.

Frontiers in immunology

Hoorweg K,Peters CP,Cornelissen F,Aparicio-Domingo P,Papazian N,Kazemier G,Mjösberg JM,Spits H,Cupedo T

Thu Aug 23 00:00:00 EDT 2012

Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation.

BMC immunology

Patel ES,Okada S,Hachey K,Yang LJ,Durum SK,Moreb JS,Chang LJ

Thu Aug 16 00:00:00 EDT 2012

Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation.

BMC immunology

Patel ES,Okada S,Hachey K,Yang LJ,Durum SK,Moreb JS,Chang LJ

Thu Aug 16 00:00:00 EDT 2012

MHC class I-presented T cell epitopes identified by immunoproteomics analysis are targets for a cross reactive influenza-specific T cell response.

PloS one

Testa JS,Shetty V,Hafner J,Nickens Z,Kamal S,Sinnathamby G,Philip R

Wed Apr 24 00:00:00 EDT 2013

Characterization of T-cell responses against IκBα in cancer patients.

Oncoimmunology

Munir S,Frøsig TM,Hansen M,Svane IM,Andersen MH

Thu Nov 01 00:00:00 EDT 2012

Signal transducer and activator of transcription 5b (Stat5b) serine 193 is a novel cytokine-induced phospho-regulatory site that is constitutively activated in primary hematopoietic malignancies.

The Journal of biological chemistry

Mitra A,Ross JA,Rodriguez G,Nagy ZS,Wilson HL,Kirken RA

Fri May 11 00:00:00 EDT 2012

Activation of STAT6 by STING is critical for antiviral innate immunity.

Cell

Chen H,Sun H,You F,Sun W,Zhou X,Chen L,Yang J,Wang Y,Tang H,Guan Y,Xia W,Gu J,Ishikawa H,Gutman D,Barber G,Qin Z,Jiang Z

Fri Oct 14 00:00:00 EDT 2011

Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the γc cytokines and TGF-β1.

Blood

Park YP,Choi SC,Kiesler P,Gil-Krzewska A,Borrego F,Weck J,Krzewski K,Coligan JE

Thu Sep 15 00:00:00 EDT 2011

Homeostatic proliferation fails to efficiently reactivate HIV-1 latently infected central memory CD4+ T cells.

PLoS pathogens

Bosque A,Famiglietti M,Weyrich AS,Goulston C,Planelles V

Sat Oct 01 00:00:00 EDT 2011

Activation of Wnt signaling arrests effector differentiation in human peripheral and cord blood-derived T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Muralidharan S,Hanley PJ,Liu E,Chakraborty R,Bollard C,Shpall E,Rooney C,Savoldo B,Rodgers J,Dotti G

Tue Nov 15 00:00:00 EST 2011

Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells.

PloS one

Sjeklocha LM,Park CW,Wong PY,Roney MJ,Belcher JD,Kaufman DS,Vercellotti GM,Hebbel RP,Steer CJ

Mon May 21 00:00:00 EDT 2012

IL-7 promotes CD95-induced apoptosis in B cells via the IFN-γ/STAT1 pathway.

PloS one

Sammicheli S,Dang VP,Ruffin N,Pham HT,Lantto R,Vivar N,Chiodi F,Rethi B

Mon Aug 27 00:00:00 EDT 2012

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

Mon Aug 27 00:00:00 EDT 2012

Day-4 myeloid dendritic cells pulsed with whole tumor lysate are highly immunogenic and elicit potent anti-tumor responses.

PloS one

Chiang CL,Hagemann AR,Leskowitz R,Mick R,Garrabrant T,Czerniecki BJ,Kandalaft LE,Powell DJ,Coukos G

Mon Aug 27 00:00:00 EDT 2012

Flt3 ligand promotes the generation of a distinct CD34(+) human natural killer cell progenitor that responds to interleukin-15.

Blood

Yu H,Fehniger TA,Fuchshuber P,Thiel KS,Vivier E,Carson WE,Caligiuri MA

Sun Nov 15 00:00:00 EST 1998

Bioinformatics

Protein Aliases: CD127; CD127 antigen; CDw127; H-IL-7; IL-7; IL-7 receptor subunit alpha; IL-7R subunit alpha; ILN; Interleukin; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7

Human IL-7, Animal-Free Recombinant Protein, PeproTech®

Human IL-7, Animal-Free Protein (AF-200-07-10UG) in Functional

Product Details

AF-200-07-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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