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Product Details
RP-88081
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Pro form of human ISG15, amino acids Met1-Ser165 (Accession # AAH09507.1)
Molecular weight
17.92 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
50mM tris, pH 8, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (650 µL) be added to the vial to prepare a stock solution of 1.54 mg/mL.
Concentration is measured by UV.
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
Gene Aliases: G1P2; hUCRP; IFI15; IMD38; IP17; ISG15; UCRP
UniProt ID: (Human) P05161
Entrez Gene ID: (Human) 9636
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