Gibco

Human MIP-1 beta (CCL4) Recombinant Protein, PeproTech®

Product Details

300-09-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 38 publications 38 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species

Human

Published species

Bacteria, Hamster, Human, Mouse, Pig

Expression System

E. coli

Amino acid sequence

APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN

Molecular weight

7.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human blood monocytes using a concentration range of 5.0-20.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human MIP-1beta is a 7.6 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The tumor suppressor MIR139 is silenced by POLR2M to promote AML oncogenesis.

Leukemia

Stavast CJ,van Zuijen I,Karkoulia E,Özçelik A,van Hoven-Beijen A,Leon LG,Voerman JSA,Janssen GMC,van Veelen PA,Burocziova M,Brouwer RWW,van IJcken WFJ,Maas A,Bindels EM,van der Velden VHJ,Schliehe C,Katsikis PD,Alberich-Jorda M,Erkeland SJ

300-09 was used in Cell Culture to investigate miRNA-139 in acute myeloid leukemia.

Tue Mar 01 00:00:00 EST 2022

G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators.

Nature communications

Gurusamy M,Tischner D,Shao J,Klatt S,Zukunft S,Bonnavion R,Günther S,Siebenbrodt K,Kestner RI,Kuhlmann T,Fleming I,Offermanns S,Wettschureck N

Tue Nov 23 00:00:00 EST 2021

T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8⁺ T cells in oral lichen planus.

Journal of cellular and molecular medicine

Yang JY,Zhang J,Lu R,Tan YQ,Du GF,Zhou G

300-09 was used in In vitro experiments to study the effects of T cell-derived exosomes on the pathogenesis of oral lichen planus and its mechanism.

Tue Dec 01 00:00:00 EST 2020

Atypical chemokine receptor ACKR2-V41A has decreased CCL2 binding, scavenging, and activation, supporting sustained inflammation and increased Alzheimer's disease risk.

Scientific reports

Murcia JDG,Weinert A,Freitas CMT,Arens DK,Ferrel MN,Grose JH,Ridge PG,Wilson E,Kauwe JSK,Weber KS

Fri May 15 00:00:00 EDT 2020

FGL2 promotes tumor progression in the CNS by suppressing CD103⁺ dendritic cell differentiation.

Nature communications

Yan J,Zhao Q,Gabrusiewicz K,Kong LY,Xia X,Wang J,Ott M,Xu J,Davis RE,Huo L,Rao G,Sun SC,Watowich SS,Heimberger AB,Li S

Fri Jan 25 00:00:00 EST 2019

Chemokines as the modulators of endometrial epithelial cells remodelling.

Scientific reports

Złotkowska A,Andronowska A

Tue Sep 10 00:00:00 EDT 2019

Chemokines as the modulators of endometrial epithelial cells remodelling.

Scientific reports

Złotkowska A,Andronowska A

Tue Sep 10 00:00:00 EDT 2019

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

Macrophage Cytokines Enhance Cell Proliferation of Normal Prostate Epithelial Cells through Activation of ERK and Akt.

Scientific reports

Dang T,Liou GY

Wed May 16 00:00:00 EDT 2018

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Dual Function of Ccr5 during Langat Virus Encephalitis: Reduction in Neutrophil-Mediated Central Nervous System Inflammation and Increase in T Cell-Mediated Viral Clearance.

Journal of immunology (Baltimore, Md. : 1950)

Michlmayr D,Bardina SV,Rodriguez CA,Pletnev AG,Lim JK

Wed Jun 01 00:00:00 EDT 2016

Sensitizing protective tumor microenvironments to antibody-mediated therapy.

Cell

Pallasch CP,Leskov I,Braun CJ,Vorholt D,Drake A,Soto-Feliciano YM,Bent EH,Schwamb J,Iliopoulou B,Kutsch N,van Rooijen N,Frenzel LP,Wendtner CM,Heukamp L,Kreuzer KA,Hallek M,Chen J,Hemann MT

Thu Jan 30 00:00:00 EST 2014

Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Epaulard O,Adam L,Poux C,Zurawski G,Salabert N,Rosenbaum P,Dereuddre-Bosquet N,Zurawski S,Flamar AL,Oh S,Romain G,Chapon C,Banchereau J,Lévy Y,Le Grand R,Martinon F

Mon Sep 01 00:00:00 EDT 2014

Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

Frontiers in pharmacology

Lee R,Perry B,Heywood J,Reese C,Bonner M,Hatfield CM,Silver RM,Visconti RP,Hoffman S,Tourkina E

300-09 was used in Immunohistochemistry-immunofluorescence to suggest that low monocyte caveolin-1 promotes fibrosis by enhancing the recruitment of fibrocytes and their progenitors into affected tissue.

Thu Jun 26 00:00:00 EDT 2014

Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Hagberg N,Theorell J,Schlums H,Eloranta ML,Bryceson YT,Rönnblom L

Sun Sep 15 00:00:00 EDT 2013

IFN-α production by plasmacytoid dendritic cells stimulated with RNA-containing immune complexes is promoted by NK cells via MIP-1β and LFA-1.

Journal of immunology (Baltimore, Md. : 1950)

Hagberg N,Berggren O,Leonard D,Weber G,Bryceson YT,Alm GV,Eloranta ML,Rönnblom L

Sun May 01 00:00:00 EDT 2011

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

Novel peptides based on HIV-1 gp120 sequence with homology to chemokines inhibit HIV infection in cell culture.

PloS one

Chertov O,Zhang N,Chen X,Oppenheim JJ,Lubkowski J,McGrath C,Sowder RC,Crise BJ,Malyguine A,Kutzler MA,Steele AD,Henderson EE,Rogers TJ

Tue Jan 11 00:00:00 EST 2011

CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.

Journal of immunology (Baltimore, Md. : 1950)

Zhang HH,Song K,Rabin RL,Hill BJ,Perfetto SP,Roederer M,Douek DC,Siegel RM,Farber JM

Wed Dec 01 00:00:00 EST 2010

CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.

Journal of immunology (Baltimore, Md. : 1950)

Carbone T,Nasorri F,Pennino D,Eyerich K,Foerster S,Cifaldi L,Traidl-Hoffman C,Behrendt H,Cavani A

Fri Jan 15 00:00:00 EST 2010

Chemokine scavenger D6 is expressed by trophoblasts and aids the survival of mouse embryos transferred into allogeneic recipients.

Journal of immunology (Baltimore, Md. : 1950)

Madigan J,Freeman DJ,Menzies F,Forrow S,Nelson SM,Young A,Sharkey A,Moffett A,Graham GJ,Greer IA,Rot A,Nibbs RJ

Mon Mar 15 00:00:00 EDT 2010

Dynamic interaction networks in a hierarchically organized tissue.

Molecular systems biology

Kirouac DC,Ito C,Csaszar E,Roch A,Yu M,Sykes EA,Bader GD,Zandstra PW

Tue Oct 05 00:00:00 EDT 2010

FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.

Journal of immunology (Baltimore, Md. : 1950)

Toda E,Terashima Y,Sato T,Hirose K,Kanegasaki S,Matsushima K

Sun Nov 15 00:00:00 EST 2009

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 EDT 2009

Microdialysis of cytokines: methodological considerations, scanning electron microscopy, and determination of relative recovery.

Journal of neurotrauma

Helmy A,Carpenter KL,Skepper JN,Kirkpatrick PJ,Pickard JD,Hutchinson PJ

Wed Apr 01 00:00:00 EDT 2009

Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Lee J,Hillsamer P,Kim CH

Tue Jan 01 00:00:00 EST 2008

CCR5-dependent homing of naturally occurring CD4+ regulatory T cells to sites of Leishmania major infection favors pathogen persistence.

The Journal of experimental medicine

Yurchenko E,Tritt M,Hay V,Shevach EM,Belkaid Y,Piccirillo CA

Mon Oct 30 00:00:00 EST 2006

CC-chemokine ligand 16 induces a novel maturation program in human immature monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cappello P,Fraone T,Barberis L,Costa C,Hirsch E,Elia AR,Caorsi C,Musso T,Novelli F,Giovarelli M

Wed Nov 01 00:00:00 EST 2006

Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Chiu WK,Fann M,Weng NP

Fri Dec 01 00:00:00 EST 2006

Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Chiu WK,Fann M,Weng NP

Fri Dec 01 00:00:00 EST 2006

Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Broxmeyer HE,Kim CH

Sat Jul 15 00:00:00 EDT 2006

Structure/function relationships of CCR8 agonists and antagonists. Amino-terminal extension of CCL1 by a single amino acid generates a partial agonist.

The Journal of biological chemistry

Fox JM,Najarro P,Smith GL,Struyf S,Proost P,Pease JE

Fri Dec 01 00:00:00 EST 2006

T-bet is required for optimal proinflammatory CD4+ T-cell trafficking.

Blood

Lord GM,Rao RM,Choe H,Sullivan BM,Lichtman AH,Luscinskas FW,Glimcher LH

Tue Nov 15 00:00:00 EST 2005

Chemokine receptor-mediated delivery directs self-tumor antigen efficiently into the class II processing pathway in vitro and induces protective immunity in vivo.

Blood

Biragyn A,Ruffini PA,Coscia M,Harvey LK,Neelapu SS,Baskar S,Wang JM,Kwak LW

Fri Oct 01 00:00:00 EDT 2004

Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus.

The Journal of biological chemistry

Hirata T,Furukawa Y,Yang BG,Hieshima K,Fukuda M,Kannagi R,Yoshie O,Miyasaka M

Fri Dec 10 00:00:00 EST 2004

Constitutive activation of CCR5 and CCR2 induced by conformational changes in the conserved TXP motif in transmembrane helix 2.

The Journal of biological chemistry

Alvarez Arias D,Navenot JM,Zhang WB,Broach J,Peiper SC

Fri Sep 19 00:00:00 EDT 2003

CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.

Blood

Honczarenko M,Le Y,Glodek AM,Majka M,Campbell JJ,Ratajczak MZ,Silberstein LE

Tue Oct 01 00:00:00 EDT 2002

The role of HIV-related chemokine receptors and chemokines in human erythropoiesis in vitro.

Stem cells (Dayton, Ohio)

Majka M,Ratajczak J,Lee B,Honczarenko M,Douglas R,Kowalska MA,Silberstein L,Gewirtz AM,Ratajczak MZ

Mon Jun 26 00:00:00 EDT 2000

Differential effects of CC chemokines on CC chemokine receptor 5 (CCR5) phosphorylation and identification of phosphorylation sites on the CCR5 carboxyl terminus.

The Journal of biological chemistry

Oppermann M,Mack M,Proudfoot AE,Olbrich H

Fri Mar 26 00:00:00 EST 1999

Chemokines and the arrest of lymphocytes rolling under flow conditions.

Science (New York, N.Y.)

Campbell JJ,Hedrick J,Zlotnik A,Siani MA,Thompson DA,Butcher EC

Fri Jan 16 00:00:00 EST 1998

Chemokine sequestration by viral chemoreceptors as a novel viral escape strategy: withdrawal of chemokines from the environment of cytomegalovirus-infected cells.

The Journal of experimental medicine

Bodaghi B,Jones TR,Zipeto D,Vita C,Sun L,Laurent L,Arenzana-Seisdedos F,Virelizier JL,Michelson S

Mon Sep 07 00:00:00 EDT 1998

HIV-1 envelope gp120 inhibits the monocyte response to chemokines through CD4 signal-dependent chemokine receptor down-regulation.

Journal of immunology (Baltimore, Md. : 1950)

Wang JM,Ueda H,Howard OM,Grimm MC,Chertov O,Gong X,Gong W,Resau JH,Broder CC,Evans G,Arthur LO,Ruscetti FW,Oppenheim JJ

Thu Oct 15 00:00:00 EDT 1998

Type I IFNs enhance the terminal differentiation of dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Luft T,Pang KC,Thomas E,Hertzog P,Hart DN,Trapani J,Cebon J

Sat Aug 15 00:00:00 EDT 1998

Identification of single C motif-1/lymphotactin receptor XCR1.

The Journal of biological chemistry

Yoshida T,Imai T,Kakizaki M,Nishimura M,Takagi S,Yoshie O

Fri Jun 26 00:00:00 EDT 1998

Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309.

The Journal of experimental medicine

Tiffany HL,Lautens LL,Gao JL,Pease J,Locati M,Combadiere C,Modi W,Bonner TI,Murphy PM

Mon Jul 07 00:00:00 EDT 1997

Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309.

The Journal of experimental medicine

Tiffany HL,Lautens LL,Gao JL,Pease J,Locati M,Combadiere C,Modi W,Bonner TI,Murphy PM

Mon Jul 07 00:00:00 EDT 1997

Cloning and characterization of a specific receptor for the novel CC chemokine MIP-3alpha from lung dendritic cells.

The Journal of experimental medicine

Power CA,Church DJ,Meyer A,Alouani S,Proudfoot AE,Clark-Lewis I,Sozzani S,Mantovani A,Wells TN

Mon Sep 15 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: ACT-2; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; G-26 T-lymphocyte-secreted protein; HC21; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1-beta; MIP-1-beta(1-69); MIP1-beta; MIP1beta; PAT 744; Protein H400; RP23-320E6.8; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Gene Aliases: ACT2; AT744.1; CCL4; G-26; HC21; LAG-1; LAG1; MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4

Human MIP-1 beta (CCL4) Recombinant Protein, PeproTech®

Product Details

300-09-10UG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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