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Product Details
140-14-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human,
Mouse,
Rat
Published species
Mouse
Expression System
E. coli
Amino acid sequence
RMSDKPDMAE IEKFDKSKLK KTETQEKNPL PSKETIEQEK QAGES
Molecular weight
5.2 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Pretreatment of primary lung fibroblasts with recombinant Thymosin-beta4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
140-14-1MG will be provided as 2 x 500 µg (140-14-500UG).
Recombinant Human/Murine/Rat Thymosin-beta4 is a 5.2 kDa glycoprotein containing 45 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Thymosin-beta4 is a small, actin-sequestering protein belonging to the thymosin-beta family that is found at high concentrations within the spleen, thymus, and peritoneal macrophages, where it is most notably responsible for the organization of cytoskeletal structure. In mammalian tissues, this protein acts as a modulator for the polymerization/depolymerization of actin through the formation of a 1:1 complex with the monomer G (globular)-actin, and inhibits actin's polymerization to form F (filamentous) actin, which together with other proteins binds microfilaments to construct the cytoskeleton. Commonly found at significant quantities within the brain, lungs, liver, kidneys, testes, and heart, thymosin-beta4 has also been shown to be synthesized by cells unrelated to the reticuloendothelial system, such as myoblasts and fibroblasts, and expressed at irregular levels by several hemopoietic cell lines, malignant lymphoid cells and myeloma cells. In addition to regulating actin polymerization, research has also found thymosin-beta4 to stimulate the secretion of hypothalamic luteinizing hormone-releasing hormone and luteinizing hormone, inhibit the migration of peritoneal macrophages, induce phenotypic changes in T cell lines during early host defense mechanisms, and inhibit the progression of hematopoietic pluripotent stem cells into the S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fx; prothymosin beta 4; prothymosin beta-4; T beta 4; T beta-4; TB4Xbeta 4; thymosin; thymosin beta 4; Thymosin beta-4; thymosin, beta 4, X chromosome
Gene Aliases: FX; PTMB4; Tb4; TB4X; THYB4; TMSB4; TMSB4X
UniProt ID: (Human) P62328, (Rat) P62329, (Mouse) P20065
Entrez Gene ID: (Human) 7114, (Rat) 81814, (Mouse) 19241
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