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Product Details
RP-75669
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Amino acids Ser2- Leu434 containing an N-terminal His-tag
Tag
His-tag
Class
Recombinant
Type
Protein
Purity
>95%, by SDS-PAGE under reducing conditions
Conjugate
Form
Liquid
Concentration
2.35 mg/mL
Purification
purified
Storage buffer
PBS with 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This product contains purified recombinant human Neuron Specific enolase protein with an N-terminal hexahistidine tag.
This product may be used as a positive control in many immunological assays.
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Eno; ENOG; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; gamma enolase; Gamma-enolase; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuron-specific enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; NSE
Gene Aliases: ENO2; HEL-S-279; NSE
UniProt ID: (Human) P09104
Entrez Gene ID: (Human) 2026
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