Gibco

Human Oncostatin M (227 aa) Recombinant Protein, PeproTech®

Product Details

300-10-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 54 publications 54 publications

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species

Human

Published species

Dog, Human, Mouse, Non-human primate, Rat

Expression System

E. coli

Amino acid sequence

AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR

Molecular weight

25.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Oncostatin M is a 25.7 kDa protein, containing 227 amino acid residues (full length precursor).

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells. OSM shares several structural and functional characteristics with LIF, IL-6, and CNTF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice.

Nature communications

Chen Y,Chen L,Wu X,Zhao Y,Wang Y,Jiang D,Liu X,Zhou T,Li S,Wei Y,Liu Y,Hu C,Zhou B,Qin J,Ying H,Ding Q

300-10 was used in In vitro experiments to identify an epigenetic mechanism by which the early phase lipid redistribution from adipose tissue to liver during regeneration impacts hepatocyte proliferation, and suggest a potential strategy to boost liver regeneration.

Sat Mar 18 00:00:00 EDT 2023

Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging.

Cell reports methods

LaLone V,Aizenshtadt A,Goertz J,Skottvoll FS,Mota MB,You J,Zhao X,Berg HE,Stokowiec J,Yu M,Schwendeman A,Scholz H,Wilson SR,Krauss S,Stevens MM

300-10 was used in In vitro experiments to develop an integrated bioanalytical methodology, qRamanomics, to qualify Raman spectral imaging as a tissue phantom calibrated tool for quantitative spatial chemotyping of major classes of biomolecules.

Mon Apr 24 00:00:00 EDT 2023

A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise.

Cell stem cell

Gadomski S,Fielding C,García-García A,Korn C,Kapeni C,Ashraf S,Villadiego J,Toro RD,Domingues O,Skepper JN,Michel T,Zimmer J,Sendtner R,Dillon S,Poole KES,Holdsworth G,Sendtner M,Toledo-Aral JJ,De Bari C,McCaskie AW,Robey PG,Méndez-Ferrer S

300-10 was used in Cell Culture to uncover a cholinergic neuro-osteocyte interface that regulates skeletogenesis and skeletal turnover through bone-anabolic effects.

Thu Apr 07 00:00:00 EDT 2022

ATP7B gene therapy of autologous reprogrammed hepatocytes alleviates copper accumulation in a mouse model of Wilson's disease.

Hepatology (Baltimore, Md.)

Cai H,Cheng X,Wang XP

Sat Oct 01 00:00:00 EDT 2022

Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway.

Stem cell research & therapy

Wu H,Tang X,Wang Y,Wang N,Chen Q,Xie J,Liu S,Zhong Z,Qiu Y,Situ P,Zern MA,Wang J,Chen H,Duan Y

300-10 was used in Cell Culture to demonstrate a significant step toward developing the expansion of Human pluripotent stem cells (hPSCs) under 3D suspension condition in large-scale cultures. The study played an important role in controlling the size of hPSC aggregates in 3D suspension culture by inhibiting the expression of ICAM1 coupled with the down-regulation of E-cad through the activation of the Wnt signaling pathway.

Thu May 26 00:00:00 EDT 2022

Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion.

iScience

Zhang Y,Guo A,Lyu C,Bi R,Wu Z,Li W,Zhao P,Niu Y,Na J,Xi JJ,Du Y

Fri Nov 19 00:00:00 EST 2021

MiR-193b-3p-ERBB4 axis regulates psoriasis pathogenesis via modulating cellular proliferation and inflammatory-mediator production of keratinocytes.

Cell death & disease

Huang C,Zhong W,Ren X,Huang X,Li Z,Chen C,Jiang B,Chen Z,Jian X,Yang L,Liu X,Huang H,Shen C,Chen X,Dou X,Yu B

Tue Oct 19 00:00:00 EDT 2021

Autologous transplantation of thecal stem cells restores ovarian function in nonhuman primates.

Cell discovery

Chen H,Xia K,Huang W,Li H,Wang C,Ma Y,Chen J,Luo P,Zheng S,Wang J,Wang Y,Dong L,Tan Z,Lai X,Mao FF,Li W,Liang X,Wang T,Xiang AP,Ke Q

Tue Aug 31 00:00:00 EDT 2021

Protocol for automated production of human stem cell derived liver spheres.

STAR protocols

Meseguer-Ripolles J,Kasarinaite A,Lucendo-Villarin B,Hay DC

Fri Jun 18 00:00:00 EDT 2021

Dimethyl fumarate reduces hepatocyte senescence following paracetamol exposure.

iScience

Meseguer-Ripolles J,Lucendo-Villarin B,Tucker C,Ferreira-Gonzalez S,Homer N,Wang Y,Starkey Lewis PJ,M Toledo E,Mellado-Gomez E,Simpson J,Flint O,Jaiswal H,Beer NL,Karlsen AE,Forbes SJ,Dear JW,Hughes J,Hay DC

Fri Jun 25 00:00:00 EDT 2021

Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes.

STAR protocols

Sinton MC,Meseguer-Ripolles J,Lucendo-Villarin B,Drake AJ,Hay DC

300-10 was used in Cell Culture to evaluate hepatocyte-like cell manufacturing from human pluripotent stem cells to study hepatic steatosis.

Fri Jun 18 00:00:00 EDT 2021

Live-cell screening platform using human-induced pluripotent stem cells expressing fluorescence-tagged cytochrome P450 1A1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kim JW,Im I,Kim H,Jeon JS,Kang EH,Jo S,Chun HS,Yoon S,Kim JH,Kim SK,Park HJ

Wed Jul 01 00:00:00 EDT 2020

Generation of multi-cellular human liver organoids from pluripotent stem cells.

Methods in cell biology

Thompson WL,Takebe T

300-10 was used in Cell Culture to demonstrate a protocol utilizing PSC derived 3D human liver organoids as a renewable, reproducible and personalized cell source, thus facilitating disease modeling and mechanistic studies.

Wed Jul 07 00:00:00 EDT 2021

Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives.

Scientific reports

Jo HY,Han HW,Jung I,Ju JH,Park SJ,Moon S,Geum D,Kim H,Park HJ,Kim S,Stacey GN,Koo SK,Park MH,Kim JH

Tue Mar 03 00:00:00 EST 2020

Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.

Scientific reports

Chen SW,Himeno M,Koui Y,Sugiyama M,Nishitsuji H,Mizokami M,Shimotohno K,Miyajima A,Kido T

Tue Sep 01 00:00:00 EDT 2020

Oncostatin M inhibits differentiation of rat stem Leydig cells in vivo and in vitro.

Journal of cellular and molecular medicine

Wang Y,Xie L,Tian E,Li X,Wen Z,Li L,Chen L,Zhong Y,Ge RS

Tue Jan 01 00:00:00 EST 2019

Oncostatin M inhibits differentiation of rat stem Leydig cells in vivo and in vitro.

Journal of cellular and molecular medicine

Wang Y,Xie L,Tian E,Li X,Wen Z,Li L,Chen L,Zhong Y,Ge RS

Tue Jan 01 00:00:00 EST 2019

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells.

Scientific reports

Pettinato G,Lehoux S,Ramanathan R,Salem MM,He LX,Muse O,Flaumenhaft R,Thompson MT,Rouse EA,Cummings RD,Wen X,Fisher RA

Thu Jun 20 00:00:00 EDT 2019

A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.

Scientific reports

Ohta S,Ikemoto T,Wada Y,Saito Y,Yamada S,Imura S,Morine Y,Shimada M

Tue Dec 10 00:00:00 EST 2019

Multiomics Analyses of HNF4α Protein Domain Function during Human Pluripotent Stem Cell Differentiation.

iScience

Wang Y,Tatham MH,Schmidt-Heck W,Swann C,Singh-Dolt K,Meseguer-Ripolles J,Lucendo-Villarin B,Kunath T,Rudd TR,Smith AJH,Hengstler JG,Godoy P,Hay RT,Hay DC

300-10 was used in Cell Culture to demonstrate that nuclear HNF4α is essential for hepatic progenitor specification, and the introduction of point mutations in HNF4α's Small Ubiquitin-like Modifier consensus motif leads to disrupted hepatocyte differentiation.

Fri Jun 28 00:00:00 EDT 2019

SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.

Nature communications

Kuroda T,Yasuda S,Tachi S,Matsuyama S,Kusakawa S,Tano K,Miura T,Matsuyama A,Sato Y

Wed May 15 00:00:00 EDT 2019

Generation of Functional Hepatocytes from Human Adipose-Derived MYC⁺ KLF4⁺ GMNN⁺ Stem Cells Analyzed by Single-Cell RNA-Seq Profiling.

Stem cells translational medicine

Li H,Zhu L,Chen H,Li T,Han Q,Wang S,Yao X,Feng H,Fan L,Gao S,Boyd R,Cao X,Zhu P,Li J,Keating A,Su X,Zhao RC

Thu Nov 01 00:00:00 EDT 2018

Cytokine-mediated modulation of the hepatic miRNome: miR-146b-5p is an IL-6-inducible miRNA with multiple targets.

Journal of leukocyte biology

Kirchmeyer M,Servais FA,Hamdorf M,Nazarov PV,Ginolhac A,Halder R,Vallar L,Glanemann M,Rubie C,Lammert F,Kreis S,Behrmann I

Thu Nov 01 00:00:00 EDT 2018

TRIM27 mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis.

Nature communications

Zhang HX,Xu ZS,Lin H,Li M,Xia T,Cui K,Wang SY,Li Y,Shu HB,Wang YY

Fri Aug 24 00:00:00 EDT 2018

Functional innate immunity restricts Hepatitis C Virus infection in induced pluripotent stem cell-derived hepatocytes.

Scientific reports

Schöbel A,Rösch K,Herker E

Thu Mar 01 00:00:00 EST 2018

Imaging-Based Screen Identifies Laminin 411 as a Physiologically Relevant Niche Factor with Importance for i-Hep Applications.

Stem cell reports

Ong J,Serra MP,Segal J,Cujba AM,Ng SS,Butler R,Millar V,Hatch S,Zimri S,Koike H,Chan K,Bonham A,Walk M,Voss T,Heaton N,Mitry R,Dhawan A,Ebner D,Danovi D,Nakauchi H,Rashid ST

Tue Mar 13 00:00:00 EDT 2018

JAK2-binding long noncoding RNA promotes breast cancer brain metastasis.

The Journal of clinical investigation

Wang S,Liang K,Hu Q,Li P,Song J,Yang Y,Yao J,Mangala LS,Li C,Yang W,Park PK,Hawke DH,Zhou J,Zhou Y,Xia W,Hung MC,Marks JR,Gallick GE,Lopez-Berestein G,Flores ER,Sood AK,Huang S,Yu D,Yang L,Lin C

Fri Dec 01 00:00:00 EST 2017

JAK2-binding long noncoding RNA promotes breast cancer brain metastasis.

The Journal of clinical investigation

Wang S,Liang K,Hu Q,Li P,Song J,Yang Y,Yao J,Mangala LS,Li C,Yang W,Park PK,Hawke DH,Zhou J,Zhou Y,Xia W,Hung MC,Marks JR,Gallick GE,Lopez-Berestein G,Flores ER,Sood AK,Huang S,Yu D,Yang L,Lin C

Fri Dec 01 00:00:00 EST 2017

A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis.

The Journal of experimental medicine

Schwerd T,Twigg SRF,Aschenbrenner D,Manrique S,Miller KA,Taylor IB,Capitani M,McGowan SJ,Sweeney E,Weber A,Chen L,Bowness P,Riordan A,Cant A,Freeman AF,Milner JD,Holland SM,Frede N,Müller M,Schmidt-Arras D,Grimbacher B,Wall SA,Jones EY,Wilkie AOM,Uhlig HH

Mon Sep 04 00:00:00 EDT 2017

Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling.

Oncogene

Junk DJ,Bryson BL,Smigiel JM,Parameswaran N,Bartel CA,Jackson MW

300-10 was used in In vitro experiments to study the specific tumor microenvironment (TME) which most actively control cancer cell plasticity for metastasis.

Thu Jul 13 00:00:00 EDT 2017

Transplanted human p75-positive stem Leydig cells replace disrupted Leydig cells for testosterone production.

Cell death & disease

Zhang M,Wang J,Deng C,Jiang MH,Feng X,Xia K,Li W,Lai X,Xiao H,Ge RS,Gao Y,Xiang AP

Thu Oct 12 00:00:00 EDT 2017

Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis.

Scientific reports

Ayaub EA,Dubey A,Imani J,Botelho F,Kolb MRJ,Richards CD,Ask K

Mon Oct 16 00:00:00 EDT 2017

GATA3 regulates FLG and FLG2 expression in human primary keratinocytes.

Scientific reports

Zeitvogel J,Jokmin N,Rieker S,Klug I,Brandenberger C,Werfel T

Tue Sep 19 00:00:00 EDT 2017

IL-33 is induced in undifferentiated, non-dividing esophageal epithelial cells in eosinophilic esophagitis.

Scientific reports

Travers J,Rochman M,Caldwell JM,Besse JA,Miracle CE,Rothenberg ME

Thu Dec 14 00:00:00 EST 2017

Adrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells.

Scientific reports

Kotaka M,Toyoda T,Yasuda K,Kitano Y,Okada C,Ohta A,Watanabe A,Uesugi M,Osafune K

Fri Dec 01 00:00:00 EST 2017

Adrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells.

Scientific reports

Kotaka M,Toyoda T,Yasuda K,Kitano Y,Okada C,Ohta A,Watanabe A,Uesugi M,Osafune K

Fri Dec 01 00:00:00 EST 2017

Xeno-sensing activity of the aryl hydrocarbon receptor in human pluripotent stem cell-derived hepatocyte-like cells.

Scientific reports

Kim HM,Kim JW,Choi Y,Chun HS,Im I,Han YM,Song CW,Yoon S,Park HJ

Mon Feb 22 00:00:00 EST 2016

STAT3-driven transcription depends upon the dimethylation of K49 by EZH2.

Proceedings of the National Academy of Sciences of the United States of America

Dasgupta M,Dermawan JK,Willard B,Stark GR

Tue Mar 31 00:00:00 EDT 2015

Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance.

Advanced healthcare materials

Villarin BL,Cameron K,Szkolnicka D,Rashidi H,Bates N,Kimber SJ,Flint O,Forbes SJ,Iredale JP,Bradley M,Hay DC

Wed Aug 26 00:00:00 EDT 2015

Small-molecule-driven hepatocyte differentiation of human pluripotent stem cells.

Stem cell reports

Siller R,Greenhough S,Naumovska E,Sullivan GJ

Tue May 12 00:00:00 EDT 2015

Mitochondrial Respiratory Defect Causes Dysfunctional Lactate Turnover via AMP-activated Protein Kinase Activation in Human-induced Pluripotent Stem Cell-derived Hepatocytes.

The Journal of biological chemistry

Im I,Jang MJ,Park SJ,Lee SH,Choi JH,Yoo HW,Kim S,Han YM

Fri Dec 04 00:00:00 EST 2015

CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.

Stem cell reports

Kido T,Koui Y,Suzuki K,Kobayashi A,Miura Y,Chern EY,Tanaka M,Miyajima A

Tue Oct 13 00:00:00 EDT 2015

Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes.

Stem cell reports

Cameron K,Tan R,Schmidt-Heck W,Campos G,Lyall MJ,Wang Y,Lucendo-Villarin B,Szkolnicka D,Bates N,Kimber SJ,Hengstler JG,Godoy P,Forbes SJ,Hay DC

Tue Dec 08 00:00:00 EST 2015

Characterization of Nestin-positive stem Leydig cells as a potential source for the treatment of testicular Leydig cell dysfunction.

Cell research

Jiang MH,Cai B,Tuo Y,Wang J,Zang ZJ,Tu X,Gao Y,Su Z,Li W,Li G,Zhang M,Jiao J,Wan Z,Deng C,Lahn BT,Xiang AP

Mon Dec 01 00:00:00 EST 2014

Leukemia propagating cells rebuild an evolving niche in response to therapy.

Cancer cell

Duan CW,Shi J,Chen J,Wang B,Yu YH,Qin X,Zhou XC,Cai YJ,Li ZQ,Zhang F,Yin MZ,Tao Y,Mi JQ,Li LH,Enver T,Chen GQ,Hong DL

Mon Jun 16 00:00:00 EDT 2014

Enabling autologous human liver regeneration with differentiated adipocyte stem cells.

Cell transplantation

Xu D,Nishimura T,Zheng M,Wu M,Su H,Sato N,Lee G,Michie S,Glenn J,Peltz G

300-10 was used in Cell Culture to determine a new method of hepatocyte-like cells development from adipocyte-like stem cells.

Tue Sep 15 00:00:00 EDT 2015

Unfolded protein response is required for the definitive endodermal specification of mouse embryonic stem cells via Smad2 and β-catenin signaling.

The Journal of biological chemistry

Xu H,Tsang KS,Wang Y,Chan JC,Xu G,Gao WQ

Fri Sep 19 00:00:00 EDT 2014

Deriving functional hepatocytes from pluripotent stem cells.

Current protocols in stem cell biology

Szkolnicka D,Farnworth SL,Lucendo-Villarin B,Hay DC

300-10 was used in Cell Culture to develop robust hepatocyte differentiation methodologies for use with pluripotent stem cells.

Fri Aug 01 00:00:00 EDT 2014

Matrix remodeling maintains embryonic stem cell self-renewal by activating Stat3.

Stem cells (Dayton, Ohio)

Przybyla LM,Theunissen TW,Jaenisch R,Voldman J

Sat Jun 01 00:00:00 EDT 2013

Preclinical characterization of GLPG0634, a selective inhibitor of JAK1, for the treatment of inflammatory diseases.

Journal of immunology (Baltimore, Md. : 1950)

Van Rompaey L,Galien R,van der Aar EM,Clement-Lacroix P,Nelles L,Smets B,Lepescheux L,Christophe T,Conrath K,Vandeghinste N,Vayssiere B,De Vos S,Fletcher S,Brys R,van 't Klooster G,Feyen JH,Menet C