Gibco

Human Osteoprotegerin (TNFRSF11B) Recombinant Protein, PeproTech®

Product Details

450-14-50UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK

Molecular weight

20 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to inhibit TRAIL-induced apoptosis of LN-18 glioblastoma cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human OPG is a soluble 20.0 kDa protein containing 174 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Macrophage GIT1 Contributes to Bone Regeneration by Regulating Inflammatory Responses in an ERK/NRF2-Dependent Way.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Zhao SJ,Liu H,Chen J,Qian DF,Kong FQ,Jie J,Yin GY,Li QQ,Fan J

450-14 was used in In vivo experiments to investigate interactions between GIT1 and macrophages in bone fracture repair.

Thu Oct 01 00:00:00 EDT 2020

Immune-mediated febrile response in female rats: Role of central hypothalamic mediators.

Scientific reports

Brito HO,Radulski D,Wilhelms DB,Stojakovic A,Brito LMO,Gil da Costa RM,Trindade E,Engblom D,Franco CRC,Zampronio AR

Thu Mar 05 00:00:00 EST 2020

Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells.

Journal of immunology (Baltimore, Md. : 1950)

Stephenson S,Care MA,Fan I,Zougman A,Westhead DR,Doody GM,Tooze RM

Fri Feb 15 00:00:00 EST 2019

Murine osteoclasts secrete serine protease HtrA1 capable of degrading osteoprotegerin in the bone microenvironment.

Communications biology

Ochiai N,Nakachi Y,Yokoo T,Ichihara T,Eriksson T,Yonemoto Y,Kato T,Ogata H,Fujimoto N,Kobayashi Y,Udagawa N,Kaku S,Ueki T,Okazaki Y,Takahashi N,Suda T

Thu Apr 16 00:00:00 EDT 2020

Transcriptomic profiling of the myeloma bone-lining niche reveals BMP signalling inhibition to improve bone disease.

Nature communications

Gooding S,Olechnowicz SWZ,Morris EV,Armitage AE,Arezes J,Frost J,Repapi E,Edwards JR,Ashley N,Waugh C,Gray N,Martinez-Hackert E,Lim PJ,Pasricha SR,Knowles H,Mead AJ,Ramasamy K,Drakesmith H,Edwards CM

Fri Oct 04 00:00:00 EDT 2019

TNF-induced osteoclastogenesis and inflammatory bone resorption are inhibited by transcription factor RBP-J.

The Journal of experimental medicine

Zhao B,Grimes SN,Li S,Hu X,Ivashkiv LB

Mon Feb 13 00:00:00 EST 2012

RANKL synthesized by articular chondrocytes contributes to juxta-articular bone loss in chronic arthritis.

Arthritis research & therapy

Martínez-Calatrava MJ,Prieto-Potín I,Roman-Blas JA,Tardio L,Largo R,Herrero-Beaumont G

Mon Jun 18 00:00:00 EDT 2012

Inhibition of BDNF in multiple myeloma blocks osteoclastogenesis via down-regulated stroma-derived RANKL expression both in vitro and in vivo.

PloS one

Ai LS,Sun CY,Zhang L,Zhou SC,Chu ZB,Qin Y,Wang YD,Zeng W,Yan H,Guo T,Chen L,Yang D,Hu Y

Wed Mar 20 00:00:00 EDT 2013

Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Knowles HJ,Cleton-Jansen AM,Korsching E,Athanasou NA

Wed Dec 01 00:00:00 EST 2010

Bone is not essential for osteoclast activation.

PloS one

Fuller K,Ross JL,Szewczyk KA,Moss R,Chambers TJ

Fri Sep 17 00:00:00 EDT 2010

Central control of fever and female body temperature by RANKL/RANK.

Nature

Hanada R,Leibbrandt A,Hanada T,Kitaoka S,Furuyashiki T,Fujihara H,Trichereau J,Paolino M,Qadri F,Plehm R,Klaere S,Komnenovic V,Mimata H,Yoshimatsu H,Takahashi N,von Haeseler A,Bader M,Kilic SS,Ueta Y,Pifl C,Narumiya S,Penninger JM

Thu Nov 26 00:00:00 EST 2009

An amperometric immunosensor for osteoproteogerin based on gold nanoparticles deposited conducting polymer.

Biosensors & bioelectronics

Singh K,Rahman MA,Son JI,Kim KC,Shim YB

Sun Jun 15 00:00:00 EDT 2008

Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Barbaroux JB,Beleut M,Brisken C,Mueller CG,Groves RW

Tue Jul 15 00:00:00 EDT 2008

EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.

The Journal of biological chemistry

Zhu J,Jia X,Xiao G,Kang Y,Partridge NC,Qin L

Fri Sep 14 00:00:00 EDT 2007

A TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption, and bone loss.

The Journal of clinical investigation

Aoki K,Saito H,Itzstein C,Ishiguro M,Shibata T,Blanque R,Mian AH,Takahashi M,Suzuki Y,Yoshimatsu M,Yamaguchi A,Deprez P,Mollat P,Murali R,Ohya K,Horne WC,Baron R

Thu Jun 01 00:00:00 EDT 2006

RANK ligand and TNF-alpha mediate acid-induced bone calcium efflux in vitro.

American journal of physiology. Renal physiology

Frick KK,LaPlante K,Bushinsky DA

Tue Nov 01 00:00:00 EST 2005

MyD88 but not TRIF is essential for osteoclastogenesis induced by lipopolysaccharide, diacyl lipopeptide, and IL-1alpha.

The Journal of experimental medicine

Sato N,Takahashi N,Suda K,Nakamura M,Yamaki M,Ninomiya T,Kobayashi Y,Takada H,Shibata K,Yamamoto M,Takeda K,Akira S,Noguchi T,Udagawa N

Mon Sep 06 00:00:00 EDT 2004

Induction of RANKL expression and osteoclast maturation by the binding of fibroblast growth factor 2 to heparan sulfate proteoglycan on rheumatoid synovial fibroblasts.

Arthritis and rheumatism

Nakano K,Okada Y,Saito K,Tanaka Y

Sun Aug 01 00:00:00 EDT 2004

Disabling of receptor activator of nuclear factor-kappaB (RANK) receptor complex by novel osteoprotegerin-like peptidomimetics restores bone loss in vivo.

The Journal of biological chemistry

Cheng X,Kinosaki M,Takami M,Choi Y,Zhang H,Murali R

Fri Feb 27 00:00:00 EST 2004

CD1-dependent dendritic cell instruction.

Nature immunology

Vincent MS,Leslie DS,Gumperz JE,Xiong X,Grant EP,Brenner MB

Sun Dec 01 00:00:00 EST 2002

CD1-mediated gamma/delta T cell maturation of dendritic cells.

The Journal of experimental medicine

Leslie DS,Vincent MS,Spada FM,Das H,Sugita M,Morita CT,Brenner MB

Mon Dec 16 00:00:00 EST 2002

Bioinformatics

Protein Aliases: MGC29565; Osteoclastogenesis inhibitory factor; Osteoprotegerin; soluble OPG; soluble Osteoprotegerin; sOPG; Tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b

Human Osteoprotegerin (TNFRSF11B) Recombinant Protein, PeproTech®

Product Details

450-14-50UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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