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Product Details
RP-88142
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human PCSK9, amino acids Met1-Gln692 (Accession # NP_777596.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
72.5 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 Protein, His Tag with an affinity constant of 16nM as determined in BLI assay. Immobilized Anti-PCSK9(Amg)-IgG2 Antibody at 2 µg/mL (100 µL/well) can bind Recombinant Human PCSK9 / NARC1 Protein (His Tag), the EC50 is 14-52 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (500 µL) be added to the vial to prepare a stock solution of 1.11 mg/mL.
Concentration is measured by UV.
Target Information
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: convertase subtilisin/kexin type 9 preproprotein; hypercholesterolemia; NARC-1; neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; OTTHUMP00000009833; PC9; Proprotein convertase 9; Proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9
Gene Aliases: FH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9; PCSK9; PSEC0052
UniProt ID: (Human) Q8NBP7
Entrez Gene ID: (Human) 255738
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