Gibco

Human TGF-alpha, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-100-16A-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Horse, Human, Rat

Expression System

E. coli

Amino acid sequence

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA

Molecular weight

5.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human TGF-alpha is a 50 amino acid polypeptide (5.5 kDa), which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini.

Cell discovery

Wang H,Huang R,Li L,Zhu J,Li Z,Peng C,Zhuang X,Lin H,Shi S,Huang P

Mon Apr 19 00:00:00 EDT 2021

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells.

Journal of visualized experiments : JoVE

Jeon K,Park K,Jetten AM

AF-100-16A was used in In vitro experiments to study inefficient and time-cosuming standard approaches for human embryonic stem cell (hESC) culture using colony type culture to maintain undifferentiated hESCs and embryoid body (EB) and rosette formation for differentiation into different germ layers to instead present a single-cell culture method using hESCs instead of a colony-type culture.

Sat Jan 18 00:00:00 EST 2020

Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma.

EMBO molecular medicine

Chua V,Orloff M,Teh JL,Sugase T,Liao C,Purwin TJ,Lam BQ,Terai M,Ambrosini G,Carvajal RD,Schwartz G,Sato T,Aplin AE

Fri Feb 01 00:00:00 EST 2019

Glutamine/glutamate metabolism rewiring in reprogrammed human hepatocyte-like cells.

Scientific reports

Ballester M,Sentandreu E,Luongo G,Santamaria R,Bolonio M,Alcoriza-Balaguer MI,Palomino-Schätzlein M,Pineda-Lucena A,Castell J,Lahoz A,Bort R

Fri Nov 29 00:00:00 EST 2019

Efficacy of Two Delivery Routes for Transplanting Human Neural Progenitor Cells (NPCs) Into the Spastic Han-Wistar Rat, a Model of Ataxia.

Cell transplantation

Uhlendorf TL,Nuryyev RL,Kopyov AO,Ochoa J,Younesi S,Cohen RW,Kopyov OV

AF-100-16A was used in Cell Culture to show that direct implantation of hNPCs reduced ataxic symptoms in the sHW rat, demonstrating that stereotactic route of stem cell delivery correlates to improved clinical outcomes.

Thu Feb 16 00:00:00 EST 2017

Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3.

Scientific reports

He H,Kuriyan AE,Su CW,Mahabole M,Zhang Y,Zhu YT,Flynn HW,Parel JM,Tseng SC

AF-100-16A was used in Cell Culture to investigate heavy chain-hyaluronic acid/pentraxin 3 inhibition of proliferative vitreoretinopathy.

Thu Mar 02 00:00:00 EST 2017

Carboxypeptidase-M is regulated by lipids and CSFs in macrophages and dendritic cells and expressed selectively in tissue granulomas and foam cells.

Laboratory investigation; a journal of technical methods and pathology

Tsakiris I,Torocsik D,Gyongyosi A,Dozsa A,Szatmari I,Szanto A,Soos G,Nemes Z,Igali L,Marton I,Takats Z,Nagy L,Dezso B

Thu Mar 01 00:00:00 EST 2012

Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model.

Stem cell research & therapy

Watts AE,Yeager AE,Kopyov OV,Nixon AJ

Thu Jan 27 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Protransforming growth factor alpha; TGF a; TGF α; TGF-alpha; TGFalpha; TGFα; Transforming growth factor; transforming growth factor, alpha