Gibco

Human TNF-beta Recombinant Protein, PeproTech®

Human TNF-beta Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human TNF-beta Protein (300-01B-20UG) in Functional

Bioassay analysis of Human TNF-beta Recombinant Protein, PeproTech® (Product # 300-01B-1MG).

Product Image Gallery

Product Details

300-01B-20UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 12 publications 12 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

Molecular weight

18.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

300-01B-1MG will be provided as 2 x 500 µg (300-01B-500UG).

Recombinant Human TNF-beta is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide-linked homotrimer structure in solution.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TSC2 regulates tumor susceptibility to TRAIL-mediated T-cell killing by orchestrating mTOR signaling.

The EMBO journal

Lin CP,Traets JJH,Vredevoogd DW,Visser NL,Peeper DS

300-01B was used in In vitro experiments to find a negative correlation between TSC2 expression and TRAIL signaling in TCGA patient cohorts.

Wed Mar 01 00:00:00 EST 2023

IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasion.

The Journal of clinical investigation

Chan LC,Li CW,Xia W,Hsu JM,Lee HH,Cha JH,Wang HL,Yang WH,Yen EY,Chang WC,Zha Z,Lim SO,Lai YJ,Liu C,Liu J,Dong Q,Yang Y,Sun L,Wei Y,Nie L,Hsu JL,Li H,Ye Q,Hassan MM,Amin HM,Kaseb AO,Lin X,Wang SC,Hung MC

Mon Jul 15 00:00:00 EDT 2019

Cell Type-Specific Interferon-γ-mediated Antagonism of KSHV Lytic Replication.

Scientific reports

Park MK,Cho H,Roh SW,Kim SJ,Myoung J

Wed Feb 20 00:00:00 EST 2019

Therapeutically targeting tumor microenvironment-mediated drug resistance in estrogen receptor-positive breast cancer.

The Journal of experimental medicine

Shee K,Yang W,Hinds JW,Hampsch RA,Varn FS,Traphagen NA,Patel K,Cheng C,Jenkins NP,Kettenbach AN,Demidenko E,Owens P,Faber AC,Golub TR,Straussman R,Miller TW

Mon Mar 05 00:00:00 EST 2018

Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiency.

The Journal of clinical investigation

Walter JE,Rosen LB,Csomos K,Rosenberg JM,Mathew D,Keszei M,Ujhazi B,Chen K,Lee YN,Tirosh I,Dobbs K,Al-Herz W,Cowan MJ,Puck J,Bleesing JJ,Grimley MS,Malech H,De Ravin SS,Gennery AR,Abraham RS,Joshi AY,Boyce TG,Butte MJ,Nadeau KC,Balboni I,Sullivan KE,Akhter J,Adeli M,El-Feky RA,El-Ghoneimy DH,Dbaibo G,Wakim R,Azzari C,Palma P,Cancrini C,Capuder K,Condino-Neto A,Costa-Carvalho BT,Oliveira JB,Roifman C,Buchbinder D,Kumanovics A,Franco JL,Niehues T,Schuetz C,Kuijpers T,Yee C,Chou J,Masaad MJ,Geha R,Uzel G,Gelman R,Holland SM,Recher M,Utz PJ,Browne SK,Notarangelo LD

Mon Nov 02 00:00:00 EST 2015

Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA.

Autophagy

Xie W,Zhang L,Jiao H,Guan L,Zha J,Li X,Wu M,Wang Z,Han J,You H

300-01B was used in In vitro experiments to reveal that the selective degradation of BBC3 underlies the prosurvival role of CMA and define a previously unappreciated proapoptotic role of IKBKB that acts through phosphorylation-mediated stabilization of BBC3, thereby promoting TNF-triggered apoptosis.

Mon Jun 20 00:00:00 EDT 2016

Comparative Biochemical and Functional Analysis of Viral and Human Secreted Tumor Necrosis Factor (TNF) Decoy Receptors.

The Journal of biological chemistry

Pontejo SM,Alejo A,Alcami A

Fri Jun 26 00:00:00 EDT 2015

A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model.

The Journal of biological chemistry

Ma L,Gong H,Zhu H,Ji Q,Su P,Liu P,Cao S,Yao J,Jiang L,Han M,Ma X,Xiong D,Luo HR,Wang F,Zhou J,Xu Y

Fri May 02 00:00:00 EDT 2014

A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model.

The Journal of biological chemistry

Ma L,Gong H,Zhu H,Ji Q,Su P,Liu P,Cao S,Yao J,Jiang L,Han M,Ma X,Xiong D,Luo HR,Wang F,Zhou J,Xu Y

Fri May 02 00:00:00 EDT 2014

β-Galactoside α2,6-sialyltranferase 1 promotes transforming growth factor-β-mediated epithelial-mesenchymal transition.

The Journal of biological chemistry

Lu J,Isaji T,Im S,Fukuda T,Hashii N,Takakura D,Kawasaki N,Gu J

Fri Dec 12 00:00:00 EST 2014

Fibroblastic reticular cells from lymph nodes attenuate T cell expansion by producing nitric oxide.

PloS one

Siegert S,Huang HY,Yang CY,Scarpellino L,Carrie L,Essex S,Nelson PJ,Heikenwalder M,Acha-Orbea H,Buckley CD,Marsland BJ,Zehn D,Luther SA

Mon Mar 26 00:00:00 EDT 2012

Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.

Journal of immunology (Baltimore, Md. : 1950)

Katakai T,Suto H,Sugai M,Gonda H,Togawa A,Suematsu S,Ebisuno Y,Katagiri K,Kinashi T,Shimizu A

Sat Nov 01 00:00:00 EDT 2008

The inhibitory receptor IRp60 (CD300a) suppresses the effects of IL-5, GM-CSF, and eotaxin on human peripheral blood eosinophils.

Blood

Munitz A,Bachelet I,Eliashar R,Moretta A,Moretta L,Levi-Schaffer F

Wed Mar 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: DAMA-25N12.13; LT-alpha; Lymphotoxin-alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF β; TNF-beta; TNFbeta; TNFβ; tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1

Human TNF-beta Recombinant Protein, PeproTech®

Human TNF-beta Protein (300-01B-20UG) in Functional

Product Details

300-01B-20UG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

In vitro Assay (IV)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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