Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Influenza A H1N1 HA (A/Puerto Rico/8/1934) His-tag Recombinant Protein
Product Details
A42599
Applications
Tested Dilution
Publications
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
Influenza A virus H1N1 (A/Puerto Rico/8/1934) hemagglutinin, amino acids Met1-Gln528 (Accession # ABD77675.1), with a C-terminal His-tag
Tag
His-tag
Molecular weight
59 kDa
Class
Recombinant
Type
Protein
Purity
>85% by SDS-PAGE
Endotoxin concentration
<1.0 EU/µg
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
0.1M Glutamic acid/20mM tris/0.1M arginine, pH 8.5, with 5% mannitol, 150mM NaCl, 10% glycerol, 0.05% CHAPS, 10% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: flu hemagglutinin; H1N1 HA; H1N1 hemagglutinin; Influenza H1N1; influenza hemagglutinin
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support