Gibco

Mouse CCL20 (MIP-3 alpha) Recombinant Protein, PeproTech®

Product Details

250-27-20UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM

Molecular weight

7.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract murine CCR6-transfected HEK293 cells using a concentration range of 0.1-10.0 ng/ml

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine MIP-3alpha is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense.

Cell stem cell

Chen M,Reed RR,Lane AP

250-27 was used in Cell Culture to establish a mechanism whereby chronic rhinosinusitis inflammation causes a functional switch of neuroepithelial stem cells from regeneration to immune defense.

Thu Oct 03 00:00:00 EDT 2019

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Systematic Screening of Chemokines to Identify Candidates to Model and Create Ectopic Lymph Node Structures for Cancer Immunotherapy.

Scientific reports

Yagawa Y,Robertson-Tessi M,Zhou SL,Anderson ARA,Mulé JJ,Mailloux AW

Wed Nov 22 00:00:00 EST 2017

CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Velázquez F,Grodecki-Pena A,Knapp A,Salvador AM,Nevers T,Croce K,Alcaide P

Mon Feb 01 00:00:00 EST 2016

CCR6-dependent positioning of memory B cells is essential for their ability to mount a recall response to antigen.

Journal of immunology (Baltimore, Md. : 1950)

Elgueta R,Marks E,Nowak E,Menezes S,Benson M,Raman VS,Ortiz C,O'Connell S,Hess H,Lord GM,Noelle R

Thu Jan 15 00:00:00 EST 2015

A Small Molecule Inhibitor of ITK and RLK Impairs Th1 Differentiation and Prevents Colitis Disease Progression.

Journal of immunology (Baltimore, Md. : 1950)

Cho HS,Shin HM,Haberstock-Debic H,Xing Y,Owens TD,Funk JO,Hill RJ,Bradshaw JM,Berg LJ

Sun Nov 15 00:00:00 EST 2015

Deficiency of β-arrestin1 ameliorates collagen-induced arthritis with impaired TH17 cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Li J,Wei B,Guo A,Liu C,Huang S,Du F,Fan W,Bao C,Pei G

Tue Apr 30 00:00:00 EDT 2013

IL-27 receptor signaling regulates CD4+ T cell chemotactic responses during infection.

Journal of immunology (Baltimore, Md. : 1950)

Gwyer Findlay E,Villegas-Mendez A,de Souza JB,Inkson CA,Shaw TN,Saris CJ,Hunter CA,Riley EM,Couper KN

Wed May 01 00:00:00 EDT 2013

Aurintricarboxylic acid ameliorates experimental autoimmune encephalomyelitis by blocking chemokine-mediated pathogenic cell migration and infiltration.

Journal of immunology (Baltimore, Md. : 1950)

Zhang F,Wei W,Chai H,Xie X

Fri Feb 01 00:00:00 EST 2013

Blockade of phosphatidylinositol 3-kinase PI3Kδ or PI3Kγ reduces IL-17 and ameliorates imiquimod-induced psoriasis-like dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Roller A,Perino A,Dapavo P,Soro E,Okkenhaug K,Hirsch E,Ji H

Thu Nov 01 00:00:00 EDT 2012

Difference in Th1 and Th17 lymphocyte adhesion to endothelium.

Journal of immunology (Baltimore, Md. : 1950)

Alcaide P,Maganto-Garcia E,Newton G,Travers R,Croce KJ,Bu DX,Luscinskas FW,Lichtman AH

Wed Feb 01 00:00:00 EST 2012

Hematopoietic lineage cell-specific protein 1 functions in concert with the Wiskott-Aldrich syndrome protein to promote podosome array organization and chemotaxis in dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Dehring DA,Clarke F,Ricart BG,Huang Y,Gomez TS,Williamson EK,Hammer DA,Billadeau DD,Argon Y,Burkhardt JK

Fri Apr 15 00:00:00 EDT 2011

Chemokines and inflammatory mediators interact to regulate adult murine neural precursor cell proliferation, survival and differentiation.

PloS one

Turbic A,Leong SY,Turnley AM

Fri Feb 10 00:00:00 EST 2012

Mesenchymal stem cells exhibit firm adhesion, crawling, spreading and transmigration across aortic endothelial cells: effects of chemokines and shear.

PloS one

Chamberlain G,Smith H,Rainger GE,Middleton J

Thu Jan 26 00:00:00 EST 2012

Specific binding and chemotactic activity of mBD4 and its functional orthologue hBD2 to CCR6-expressing cells.

The Journal of biological chemistry

Röhrl J,Yang D,Oppenheim JJ,Hehlgans T

Fri Mar 05 00:00:00 EST 2010

T cell specific adapter protein (TSAd) interacts with Tec kinase ITK to promote CXCL12 induced migration of human and murine T cells.

PloS one

Berge T,Sundvold-Gjerstad V,Granum S,Andersen TC,Holthe GB,Claesson-Welsh L,Andreotti AH,Inngjerdingen M,Spurkland A

Thu Mar 18 00:00:00 EDT 2010

Inhibition of the SDF-1alpha-CXCR4 axis by the CXCR4 antagonist AMD3100 suppresses the migration of cultured cells from ATL patients and murine lymphoblastoid cells from HTLV-I Tax transgenic mice.

Blood

Kawaguchi A,Orba Y,Kimura T,Iha H,Ogata M,Tsuji T,Ainai A,Sata T,Okamoto T,Hall WW,Sawa H,Hasegawa H

Thu Oct 01 00:00:00 EDT 2009

CCR6 is required for IL-23-induced psoriasis-like inflammation in mice.

The Journal of clinical investigation

Hedrick MN,Lonsdorf AS,Shirakawa AK,Richard Lee CC,Liao F,Singh SP,Zhang HH,Grinberg A,Love PE,Hwang ST,Farber JM

Sat Aug 01 00:00:00 EDT 2009

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 EDT 2009

Structure and function of A41, a vaccinia virus chemokine binding protein.

PLoS pathogens

Bahar MW,Kenyon JC,Putz MM,Abrescia NG,Pease JE,Wise EL,Stuart DI,Smith GL,Grimes JM

Tue Jan 01 00:00:00 EST 2008

Chemokine receptor-mediated delivery directs self-tumor antigen efficiently into the class II processing pathway in vitro and induces protective immunity in vivo.

Blood

Biragyn A,Ruffini PA,Coscia M,Harvey LK,Neelapu SS,Baskar S,Wang JM,Kwak LW

Fri Oct 01 00:00:00 EDT 2004

Induction of potent antitumor immunity by in situ targeting of intratumoral DCs.

The Journal of clinical investigation

Furumoto K,Soares L,Engleman EG,Merad M

Mon Mar 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: beta chemokine exodus-1; Beta-chemokine exodus-1; C-C motif chemokine 20; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; M-MIP-3-alpha; Macrophage inflammatory protein 3 alpha; MIP 3 a; MIP 3 α; MIP 3alpha; MIP 3α; MIP-3-alpha; MIP3 a; MIP3 alpha; MIP3 α; small inducible cytokine subfamily A20; Small-inducible cytokine A20

View more View less

Gene Aliases: Ccl20; CKb4; exodus-1; LARC; MIP-3[a]; MIP-3A; MIP3A; Scya20; ST38

Mouse CCL20 (MIP-3 alpha) Recombinant Protein, PeproTech®

Product Details

250-27-20UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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