Gibco

Mouse CCL5 (RANTES) Recombinant Protein, PeproTech®

Product Details

250-07-20UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 36 publications 36 publications

Miscellaneous PubMed (Misc)

View 11 publications 11 publications

Product Specifications

Species

Mouse

Published species

Bacteria, Human, Mouse

Expression System

E. coli

Amino acid sequence

SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS

Molecular weight

7.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human activated T cells cultured in the presence of IL-2 using a concentration range of 10-30 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine RANTES is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Notch-Regulated Dendritic Cells Restrain Inflammation-Associated Colorectal Carcinogenesis.

Cancer immunology research

Wang L,Yu S,Chan ER,Chen KY,Liu C,Che D,Awadallah A,Myers J,Askew D,Huang AY,Maillard I,Huang D,Xin W,Zhou L

250-07 was used in Sample Preparation (FC/FACS) to study the significance of Notch-regulated dendritic cell (DC) immune responses in a mouse model of colitis-associated colorectal cancer in which there is epithelial downregulation of Notch/Hes1 signaling.

Mon Mar 01 00:00:00 EST 2021

Metabolic modulation by CDK4/6 inhibitor promotes chemokine-mediated recruitment of T cells into mammary tumors.

Cell reports

Uzhachenko RV,Bharti V,Ouyang Z,Blevins A,Mont S,Saleh N,Lawrence HA,Shen C,Chen SC,Ayers GD,DeNardo DG,Arteaga C,Richmond A,Vilgelm AE

Tue Apr 06 00:00:00 EDT 2021

Neddylation pathway alleviates chronic pancreatitis by reducing HIF1α-CCL5-dependent macrophage infiltration.

Cell death & disease

Lin Y,Chen Y,Feng W,Hua R,Zhang J,Huo Y,Jiang H,Yin B,Yang X

Mon Mar 15 00:00:00 EDT 2021

Aging-induced IL27Ra signaling impairs hematopoietic stem cells.

Blood

He H,Xu P,Zhang X,Liao M,Dong Q,Cong T,Tang B,Yang X,Ye M,Chang Y,Liu W,Wang X,Ju Z,Wang J

Thu Jul 09 00:00:00 EDT 2020

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

250-07 was used in In vitro experiments to reveal independent emergent behaviour in Cytotoxic T lymphocytes populations attacking tumour masses.

Tue Oct 13 00:00:00 EDT 2020

CCL5 secreted by senescent theca-interstitial cells inhibits preantral follicular development via granulosa cellular apoptosis.

Journal of cellular physiology

Shen L,Chen Y,Cheng J,Yuan S,Zhou S,Yan W,Liu J,Luo A,Wang S

250-07 was used in In vitro experiments to suggest that SASP-associated CCL5 produced by senescent theca-interstitial cells may impair follicle development and maturation during ovarian aging by promoting granulosa cell apoptosis.

Sun Dec 01 00:00:00 EST 2019

MicroRNA-155 Modulates Acute Graft-versus-Host Disease by Impacting T Cell Expansion, Migration, and Effector Function.

Journal of immunology (Baltimore, Md. : 1950)

Zitzer NC,Snyder K,Meng X,Taylor PA,Efebera YA,Devine SM,Blazar BR,Garzon R,Ranganathan P

Fri Jun 15 00:00:00 EDT 2018

Human Binge Alcohol Intake Inhibits TLR4-MyD88 and TLR4-TRIF Responses but Not the TLR3-TRIF Pathway: HspA1A and PP1 Play Selective Regulatory Roles.

Journal of immunology (Baltimore, Md. : 1950)

Muralidharan S,Lim A,Catalano D,Mandrekar P

Sun Apr 01 00:00:00 EDT 2018

Sipa1 deficiency unleashes a host-immune mechanism eradicating chronic myelogenous leukemia-initiating cells.

Nature communications

Xu Y,Ikeda S,Sumida K,Yamamoto R,Tanaka H,Minato N

Fri Mar 02 00:00:00 EST 2018

Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.

Nature communications

Wang Q,He Z,Huang M,Liu T,Wang Y,Xu H,Duan H,Ma P,Zhang L,Zamvil SS,Hidalgo J,Zhang Z,O'Rourke DM,Dahmane N,Brem S,Mou Y,Gong Y,Fan Y

Thu Feb 08 00:00:00 EST 2018

Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues.

Oncotarget

Muccioli M,Nandigam H,Loftus T,Singh M,Venkatesh A,Wright J,Pate M,McCall K,Benencia F

250-07 was used in Enzyme-linked immunosorbent assay to investigate the potential of mouse and human ovarian cancer cells to produce inflammatory factors in response to pathogen recognition receptor signalling.

Fri Nov 30 00:00:00 EST 2018

CCL5 Promotes Resolution-Phase Macrophage Reprogramming in Concert with the Atypical Chemokine Receptor D6 and Apoptotic Polymorphonuclear Cells.

Journal of immunology (Baltimore, Md. : 1950)

Aswad M,Assi S,Schif-Zuck S,Ariel A

Tue Aug 15 00:00:00 EDT 2017

Platelets Mediate Host Defense against Staphylococcus aureus through Direct Bactericidal Activity and by Enhancing Macrophage Activities.

Journal of immunology (Baltimore, Md. : 1950)

Ali RA,Wuescher LM,Dona KR,Worth RG

Sun Jan 01 00:00:00 EST 2017

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Systematic Screening of Chemokines to Identify Candidates to Model and Create Ectopic Lymph Node Structures for Cancer Immunotherapy.

Scientific reports

Yagawa Y,Robertson-Tessi M,Zhou SL,Anderson ARA,Mulé JJ,Mailloux AW

Wed Nov 22 00:00:00 EST 2017

C-C Chemokine Ligand-5 is critical for facilitating macrophage infiltration in the early phase of liver ischemia/reperfusion injury.

Scientific reports

Lee CM,Peng HH,Yang P,Liou JT,Liao CC,Day YJ

250-07 was used in In vivo experiments to investigate CCL5 in hepatic ischemia/reperfusion injury.

Fri Jun 16 00:00:00 EDT 2017

Hepatocyte Growth Factor Receptor c-Met Instructs T Cell Cardiotropism and Promotes T Cell Migration to the Heart via Autocrine Chemokine Release.

Immunity

Komarowska I,Coe D,Wang G,Haas R,Mauro C,Kishore M,Cooper D,Nadkarni S,Fu H,Steinbruchel DA,Pitzalis C,Anderson G,Bucy P,Lombardi G,Breckenridge R,Marelli-Berg FM

Tue Jun 16 00:00:00 EDT 2015

CCR6-dependent positioning of memory B cells is essential for their ability to mount a recall response to antigen.

Journal of immunology (Baltimore, Md. : 1950)

Elgueta R,Marks E,Nowak E,Menezes S,Benson M,Raman VS,Ortiz C,O'Connell S,Hess H,Lord GM,Noelle R

Thu Jan 15 00:00:00 EST 2015

Primary Macrophage Chemotaxis Induced by Cannabinoid Receptor 2 Agonists Occurs Independently of the CB2 Receptor.

Scientific reports

Taylor L,Christou I,Kapellos TS,Buchan A,Brodermann MH,Gianella-Borradori M,Russell A,Iqbal AJ,Greaves DR

Tue Jun 02 00:00:00 EDT 2015

Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens.

The Journal of biological chemistry

Bufe B,Schumann T,Kappl R,Bogeski I,Kummerow C,Podgórska M,Smola S,Hoth M,Zufall F

Fri Mar 20 00:00:00 EDT 2015

RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.

Nature communications

Patel J,McNeill E,Douglas G,Hale AB,de Bono J,Lee R,Iqbal AJ,Regan-Komito D,Stylianou E,Greaves DR,Channon KM

Wed Mar 18 00:00:00 EDT 2015

Hydrodynamic Gene Delivery of CC Chemokine Binding Fc Fusion Proteins to Target Acute Vascular Inflammation In Vivo.

Scientific reports

McNeill E,Iqbal AJ,White GE,Patel J,Greaves DR,Channon KM

Tue Dec 01 00:00:00 EST 2015

Macrophage-secreted cytokines drive pancreatic acinar-to-ductal metaplasia through NF-κB and MMPs.

The Journal of cell biology

Liou GY,Döppler H,Necela B,Krishna M,Crawford HC,Raimondo M,Storz P

Mon Aug 05 00:00:00 EDT 2013

IL-27 receptor signaling regulates CD4+ T cell chemotactic responses during infection.

Journal of immunology (Baltimore, Md. : 1950)

Gwyer Findlay E,Villegas-Mendez A,de Souza JB,Inkson CA,Shaw TN,Saris CJ,Hunter CA,Riley EM,Couper KN

Wed May 01 00:00:00 EDT 2013

Aurintricarboxylic acid ameliorates experimental autoimmune encephalomyelitis by blocking chemokine-mediated pathogenic cell migration and infiltration.

Journal of immunology (Baltimore, Md. : 1950)

Zhang F,Wei W,Chai H,Xie X

Fri Feb 01 00:00:00 EST 2013

High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses.

The Journal of experimental medicine

Yang D,Postnikov YV,Li Y,Tewary P,de la Rosa G,Wei F,Klinman D,Gioannini T,Weiss JP,Furusawa T,Bustin M,Oppenheim JJ

Mon Jan 16 00:00:00 EST 2012

Chemokine receptor Ccr1 drives neutrophil-mediated kidney immunopathology and mortality in invasive candidiasis.

PLoS pathogens

Lionakis MS,Fischer BG,Lim JK,Swamydas M,Wan W,Richard Lee CC,Cohen JI,Scheinberg P,Gao JL,Murphy PM

Mon Dec 17 00:00:00 EST 2012

Tumor-infiltrating monocytic myeloid-derived suppressor cells mediate CCR5-dependent recruitment of regulatory T cells favoring tumor growth.

Journal of immunology (Baltimore, Md. : 1950)

Schlecker E,Stojanovic A,Eisen C,Quack C,Falk CS,Umansky V,Cerwenka A

Sat Dec 15 00:00:00 EST 2012

Growth differentiation factor 15 deficiency protects against atherosclerosis by attenuating CCR2-mediated macrophage chemotaxis.

The Journal of experimental medicine

de Jager SC,Bermúdez B,Bot I,Koenen RR,Bot M,Kavelaars A,de Waard V,Heijnen CJ,Muriana FJ,Weber C,van Berkel TJ,Kuiper J,Lee SJ,Abia R,Biessen EA

Mon Feb 14 00:00:00 EST 2011

An aberrant thymus in CCR5-/- mice is coupled with an enhanced adaptive immune response in fungal infection.

Journal of immunology (Baltimore, Md. : 1950)

Kroetz DN,Deepe GS

Sun May 15 00:00:00 EDT 2011

CCL5 neutralization restricts cancer growth and potentiates the targeting of PDGFRβ in colorectal carcinoma.

PloS one

Cambien B,Richard-Fiardo P,Karimdjee BF,Martini V,Ferrua B,Pitard B,Schmid-Antomarchi H,Schmid-Alliana A

Mon Apr 23 00:00:00 EDT 2012

Thymic development beyond beta-selection requires phosphatidylinositol 3-kinase activation by CXCR4.

The Journal of experimental medicine

Janas ML,Varano G,Gudmundsson K,Noda M,Nagasawa T,Turner M

Mon Jan 18 00:00:00 EST 2010

Interferon-inducible CXC chemokines directly contribute to host defense against inhalational anthrax in a murine model of infection.

PLoS pathogens

Crawford MA,Burdick MD,Glomski IJ,Boyer AE,Barr JR,Mehrad B,Strieter RM,Hughes MA

Thu Nov 18 00:00:00 EST 2010

Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity.

Journal of immunology (Baltimore, Md. : 1950)

Arthur JC,Lich JD,Ye Z,Allen IC,Gris D,Wilson JE,Schneider M,Roney KE,O'Connor BP,Moore CB,Morrison A,Sutterwala FS,Bertin J,Koller BH,Liu Z,Ting JP

Fri Oct 15 00:00:00 EDT 2010

A cell-impermeable cyclosporine A derivative reduces pathology in a mouse model of allergic lung inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Balsley MA,Malesevic M,Stemmy EJ,Gigley J,Jurjus RA,Herzog D,Bukrinsky MI,Fischer G,Constant SL

Wed Dec 15 00:00:00 EST 2010

Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Buatois V,Fagète S,Magistrelli G,Chatel L,Fischer N,Kosco-Vilbois MH,Ferlin WG

Sun Aug 15 00:00:00 EDT 2010

CCR5 expression levels influence NFAT translocation, IL-2 production, and subsequent signaling events during T lymphocyte activation.

Journal of immunology (Baltimore, Md. : 1950)

Camargo JF,Quinones MP,Mummidi S,Srinivas S,Gaitan AA,Begum K,Jimenez F,VanCompernolle S,Unutmaz D,Ahuja SS,Ahuja SK

Thu Jan 01 00:00:00 EST 2009

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 EDT 2009

The chemokine receptor CCR5 plays a key role in the early memory CD8+ T cell response to respiratory virus infections.

Immunity

Kohlmeier JE,Miller SC,Smith J,Lu B,Gerard C,Cookenham T,Roberts AD,Woodland DL

Fri Jul 18 00:00:00 EDT 2008

Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation.

Cell stem cell

Di Giorgio FP,Boulting GL,Bobrowicz S,Eggan KC

Thu Dec 04 00:00:00 EST 2008

CXCL10 blockade protects mice from cyclophosphamide-induced cystitis.

Journal of immune based therapies and vaccines

Sakthivel SK,Singh UP,Singh S,Taub DD,Novakovic KR,Lillard JW

Tue Oct 28 00:00:00 EDT 2008

Autoantigens signal through chemokine receptors: uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate.

Blood

Howard OM,Dong HF,Su SB,Caspi RR,Chen X,Plotz P,Oppenheim JJ

Wed Jun 01 00:00:00 EDT 2005

Critical role for CCR5 in the function of donor CD4+CD25+ regulatory T cells during acute graft-versus-host disease.

Blood

Wysocki CA,Jiang Q,Panoskaltsis-Mortari A,Taylor PA,McKinnon KP,Su L,Blazar BR,Serody JS

Tue Nov 01 00:00:00 EST 2005

Leukotriene B4, an activation product of mast cells, is a chemoattractant for their progenitors.

The Journal of experimental medicine

Weller CL,Collington SJ,Brown JK,Miller HR,Al-Kashi A,Clark P,Jose PJ,Hartnell A,Williams TJ

Mon Jun 20 00:00:00 EDT 2005

CCR1 and CC chemokine ligand 5 interactions exacerbate innate immune responses during sepsis.

Journal of immunology (Baltimore, Md. : 1950)

Ness TL,Carpenter KJ,Ewing JL,Gerard CJ,Hogaboam CM,Kunkel SL

Wed Dec 01 00:00:00 EST 2004

Hematopoietic stem cells are uniquely selective in their migratory response to chemokines.

The Journal of experimental medicine

Wright DE,Bowman EP,Wagers AJ,Butcher EC,Weissman IL

Mon May 06 00:00:00 EDT 2002

The intestinal chemokine thymus-expressed chemokine (CCL25) attracts IgA antibody-secreting cells.

The Journal of experimental medicine

Bowman EP,Kuklin NA,Youngman KR,Lazarus NH,Kunkel EJ,Pan J,Greenberg HB,Butcher EC

Mon Jan 21 00:00:00 EST 2002

Homeostatic T cell proliferation in a T cell-dendritic cell coculture system.

Proceedings of the National Academy of Sciences of the United States of America

Ge Q,Palliser D,Eisen HN,Chen J

Tue Mar 05 00:00:00 EST 2002

CCL7 and CXCL10 orchestrate oxidative stress-induced neutrophilic lung inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Michalec L,Choudhury BK,Postlethwait E,Wild JS,Alam R,Lett-Brown M,Sur S

Tue Jan 15 00:00:00 EST 2002

Developmental switches in chemokine response profiles during B cell differentiation and maturation.

The Journal of experimental medicine

Bowman EP,Campbell JJ,Soler D,Dong Z,Manlongat N,Picarella D,Hardy RR,Butcher EC

Mon Apr 17 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: C-C motif chemokine 5; MGC17164; MuRantes; OTTHUMP00000197106; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; Small-inducible cytokine A5; T-cell-specific protein RANTES

Mouse CCL5 (RANTES) Recombinant Protein, PeproTech®

Product Details

250-07-20UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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