Gibco

Mouse EGF, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-315-09-500UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 9 publications 9 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Molecular weight

6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development.

Communications biology

Freitag K,Eede P,Ivanov A,Sterczyk N,Schneeberger S,Borodina T,Sauer S,Beule D,Heppner FL

AF-315-09 was used in Cell Culture to highlight the need to carefully consider the choice of astrocyte origin and phenotype with respect to age, isolation and culture protocols based on the respective biological question.

Fri Jan 13 00:00:00 EST 2023

Characterization of an in vitro 3D intestinal organoid model by using massive RNAseq-based transcriptome profiling.

Scientific reports

Lu J,Krepelova A,Rasa SMM,Annunziata F,Husak O,Adam L,Nunna S,Neri F

Tue Aug 17 00:00:00 EDT 2021

An exercise "sweet spot" reverses cognitive deficits of aging by growth-hormone-induced neurogenesis.

iScience

Blackmore DG,Steyn FJ,Carlisle A,O'Keeffe I,Vien KY,Zhou X,Leiter O,Jhaveri D,Vukovic J,Waters MJ,Bartlett PF

Fri Nov 19 00:00:00 EST 2021

Hypothalamic Rax⁺ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice.

Nature communications

Mu W,Li S,Xu J,Guo X,Wu H,Chen Z,Qiao L,Helfer G,Lu F,Liu C,Wu QF

Fri Apr 16 00:00:00 EDT 2021

Inhibition of Apoptosis Reduces Diploidization of Haploid Mouse Embryonic Stem Cells during Differentiation.

Stem cell reports

Zhang W,Tian Y,Gao Q,Li X,Li Y,Zhang J,Yao C,Wang Y,Wang H,Zhao Y,Zhang Q,Li L,Yu Y,Fan Y,Shuai L

Tue Jul 14 00:00:00 EDT 2020

Regulation of colonic epithelial cell homeostasis by mTORC1.

Scientific reports

Kotani T,Setiawan J,Konno T,Ihara N,Okamoto S,Saito Y,Murata Y,Noda T,Matozaki T

Fri Aug 14 00:00:00 EDT 2020

Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes.

Nature communications

Matsumura K,Seiriki K,Okada S,Nagase M,Ayabe S,Yamada I,Furuse T,Shibuya H,Yasuda Y,Yamamori H,Fujimoto M,Nagayasu K,Yamamoto K,Kitagawa K,Miura H,Gotoda-Nishimura N,Igarashi H,Hayashida M,Baba M,Kondo M,Hasebe S,Ueshima K,Kasai A,Ago Y,Hayata-Takano A,Shintani N,Iguchi T,Sato M,Yamaguchi S,Tamura M,Wakana S,Yoshiki A,Watabe AM,Okano H,Takuma K,Hashimoto R,Hashimoto H,Nakazawa T

Wed Feb 26 00:00:00 EST 2020

Chemical compound cinobufotalin potently induces FOXO1-stimulated cisplatin sensitivity by antagonizing its binding partner MYH9.

Signal transduction and targeted therapy

Li Y,Liu X,Lin X,Zhao M,Xiao Y,Liu C,Liang Z,Lin Z,Yi R,Tang Z,Liu J,Li X,Jiang Q,Li L,Xie Y,Liu Z,Fang W

Fri Jan 29 00:00:00 EST 2021

Boosting bioluminescence neuroimaging: an optimized protocol for brain studies.

PloS one

Aswendt M,Adamczak J,Couillard-Despres S,Hoehn M

Tue Aug 20 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: EGF; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)