Gibco

Mouse TNF-alpha, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-315-01A-20UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Molecular weight

17.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine TNF-alpha is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Obeticholic acid improved triptolide/lipopolysaccharide-induced hepatotoxicity by inhibiting caspase-11-GSDMD pyroptosis pathway.

Journal of applied toxicology : JAT

Liang P,Zhou S,Yuan Z,Zhang L,Jiang Z,Yu Q

AF-315-01A was used in In vitro experiments to indicate that FXR might be an attractive therapeutic target for TP/LPS-induced hepatotoxicity, providing an effective strategy for drug-induced liver injury.

Sat Apr 01 00:00:00 EDT 2023

Bclaf1 regulates c-FLIP expression and protects cells from TNF-induced apoptosis and tissue injury.

EMBO reports

Zhang R,Xue T,Shao A,Lang Y,Qin C,Zhao M,Kuang Y,Yu Z,Geng Y,Zhao C,Tang J

AF-315-01A was used in In vivo experiments to suggest Bclaf1 is a key regulator in TNF-induced apoptosis, both in vitro and in vivo.

Wed Jan 05 00:00:00 EST 2022

Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice.

Bioscience trends

Zhou Y,Cheng Y,Li Y,Ma J,Wu Z,Chen Y,Mei J,Chen M

AF-315-01A was used in Sample Prep (WB) to explore the relation of GABAergic transmission and soluble Aβ in early APP/PS1 mice.

Sun Nov 21 00:00:00 EST 2021

CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype.

Journal of cellular and molecular medicine

Xie C,Ye F,Zhang N,Huang Y,Pan Y,Xie X

AF-315-01A was used in In vitro experiments to investigate chemokine activation of inflammation in abdominal aortic aneurysm.

Sun Aug 01 00:00:00 EDT 2021

MiR-1290 promotes myoblast differentiation and protects against myotube atrophy via Akt/p70/FoxO3 pathway regulation.

Skeletal muscle

Che J,Xu C,Wu Y,Jia P,Han Q,Ma Y,Wang X,Zheng Y

AF-315-01A was used in In vitro experiments to investigate miRNA-1290 in sarcopenia.

Mon Mar 15 00:00:00 EDT 2021

Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology.

Nature communications

Sonner JK,Keil M,Falk-Paulsen M,Mishra N,Rehman A,Kramer M,Deumelandt K,Röwe J,Sanghvi K,Wolf L,von Landenberg A,Wolff H,Bharti R,Oezen I,Lanz TV,Wanke F,Tang Y,Brandao I,Mohapatra SR,Epping L,Grill A,Röth R,Niesler B,Meuth SG,Opitz CA,Okun JG,Reinhardt C,Kurschus FC,Wick W,Bode HB,Rosenstiel P,Platten M

Fri Oct 25 00:00:00 EDT 2019

Silencing of PTGS2 exerts promoting effects on angiogenesis endothelial progenitor cells in mice with ischemic stroke via repression of the NF-κB signaling pathway.

Journal of cellular physiology

Zhou Z,Lu C,Meng S,Dun L,Yin N,An H,Xu H,Liu G,Cai Y

AF-315-01A was used in In vitro experiments to conclude that the specific knockdown of PTGS2 expression could repress the NF-κB signaling pathway, thereby inhibits apoptosis and promotes proliferation, migration and angiogenesis of EPCs, providing protective effect on mice with ischemic stroke.

Sun Dec 01 00:00:00 EST 2019

Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock.

Immunity

Mandal P,Feng Y,Lyons JD,Berger SB,Otani S,DeLaney A,Tharp GK,Maner-Smith K,Burd EM,Schaeffer M,Hoffman S,Capriotti C,Roback L,Young CB,Liang Z,Ortlund EA,DiPaolo NC,Bosinger S,Bertin J,Gough PJ,Brodsky IE,Coopersmith CM,Shayakhmetov DM,Mocarski ES

Tue Jul 17 00:00:00 EDT 2018

CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.

Journal of immunology (Baltimore, Md. : 1950)

Dai Z,Xing L,Cerise J,Wang EH,Jabbari A,de Jong A,Petukhova L,Christiano AM,Clynes R

Mon Aug 15 00:00:00 EDT 2016

Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity.

Scientific reports

Pepin M,Mezouar S,Pegon J,Muczynski V,Adam F,Bianchini EP,Bazaa A,Proulle V,Rupin A,Paysant J,Panicot-Dubois L,Christophe OD,Dubois C,Lenting PJ,Denis CV

Mon Nov 28 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha