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Mouse TPO (Thrombopoietin), Animal-Free Recombinant Protein, PeproTech®

Mouse TPO (Thrombopoietin), Animal-Free Protein in Functional Assay (Functional)

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Mouse TPO (Thrombopoietin), Animal-Free Protein (AF-315-14-10UG) in Functional

Bioassay analysis of Mouse TPO (Thrombopoietin), Animal-Free Recombinant Protein, PeproTech® (Product # AF-315-14-1MG).

Product Image Gallery

Product Details

AF-315-14-10UG

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Mouse

Published species

Mouse

Expression System

E. coli

Amino acid sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

Molecular weight

18.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Platelets are indispensable for alveolar development in neonatal mice.

Frontiers in pediatrics

Huang Z,Lin B,Han D,Wang X,Zhong J,Wagenaar GTM,Yang C,Chen X

AF-315-14 was used in Immunohistochemistry to investigate the role of anti-CD41 antibody-induced platelet depletion during postnatal lung development mice.

Sat Aug 27 00:00:00 EDT 2022

Oncogenic ZMYND11-MBTD1 fusion protein anchors the NuA4/TIP60 histone acetyltransferase complex to the coding region of active genes.

Cell reports

Devoucoux M,Fort V,Khelifi G,Xu J,Alerasool N,Galloy M,Wong N,Bourriquen G,Fradet-Turcotte A,Taipale M,Hope K,Hussein SMI,Côté J

AF-315-14 was used in In vitro experiments to indicate that the ZMYND11-MBTD1 fusion functions primarily by mistargeting the NuA4/TIP60 complex to the body of genes, altering normal transcription of specific genes, likely driving oncogenesis in part through the Myc regulatory network.

Tue Jun 14 00:00:00 EDT 2022

Aged skeletal stem cells generate an inflammatory degenerative niche.

Nature

Ambrosi TH,Marecic O,McArdle A,Sinha R,Gulati GS,Tong X,Wang Y,Steininger HM,Hoover MY,Koepke LS,Murphy MP,Sokol J,Seo EY,Tevlin R,Lopez M,Brewer RE,Mascharak S,Lu L,Ajanaku O,Conley SD,Seita J,Morri M,Neff NF,Sahoo D,Yang F,Weissman IL,Longaker MT,Chan CKF

Wed Sep 01 00:00:00 EDT 2021

Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells.

Nature communications

Ochi K,Morita M,Wilkinson AC,Iwama A,Yamazaki S

Fri Jun 11 00:00:00 EDT 2021

Long-term ex vivo expansion of mouse hematopoietic stem cells.

Nature protocols

Wilkinson AC,Ishida R,Nakauchi H,Yamazaki S

Sat Feb 01 00:00:00 EST 2020

SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness.

Scientific reports

Nguyen CH,Glüxam T,Schlerka A,Bauer K,Grandits AM,Hackl H,Dovey O,Zöchbauer-Müller S,Cooper JL,Vassiliou GS,Stoiber D,Wieser R,Heller G

Mon Jun 24 00:00:00 EDT 2019

Stress hematopoiesis reveals abnormal control of self-renewal, lineage bias, and myeloid differentiation in Mll partial tandem duplication (Mll-PTD) hematopoietic stem/progenitor cells.

Blood

Zhang Y,Yan X,Sashida G,Zhao X,Rao Y,Goyama S,Whitman SP,Zorko N,Bernot K,Conway RM,Witte D,Wang QF,Tenen DG,Xiao Z,Marcucci G,Mulloy JC,Grimes HL,Caligiuri MA,Huang G

Thu Aug 02 00:00:00 EDT 2012

Dual actions of Meis1 inhibit erythroid progenitor development and sustain general hematopoietic cell proliferation.

Blood

Cai M,Langer EM,Gill JG,Satpathy AT,Albring JC,KC W,Murphy TL,Murphy KM

Thu Jul 12 00:00:00 EDT 2012

Stromal SPARC contributes to the detrimental fibrotic changes associated with myeloproliferation whereas its deficiency favors myeloid cell expansion.

Blood

Tripodo C,Sangaletti S,Guarnotta C,Piccaluga PP,Cacciatore M,Giuliano M,Franco G,Chiodoni C,Sciandra M,Miotti S,Calvaruso G,Carè A,Florena AM,Scotlandi K,Orazi A,Pileri SA,Colombo MP

Thu Oct 25 00:00:00 EDT 2012

Thrombopoietin/MPL participates in initiating and maintaining RUNX1-ETO acute myeloid leukemia via PI3K/AKT signaling.

Blood

Pulikkan JA,Madera D,Xue L,Bradley P,Landrette SF,Kuo YH,Abbas S,Zhu LJ,Valk P,Castilla LH

Thu Jul 26 00:00:00 EDT 2012

Activated Gs signaling in osteoblastic cells alters the hematopoietic stem cell niche in mice.

Blood

Schepers K,Hsiao EC,Garg T,Scott MJ,Passegué E

Thu Oct 25 00:00:00 EDT 2012

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

Thu Dec 13 00:00:00 EST 2012

Connexin-43 prevents hematopoietic stem cell senescence through transfer of reactive oxygen species to bone marrow stromal cells.

Proceedings of the National Academy of Sciences of the United States of America

Taniguchi Ishikawa E,Gonzalez-Nieto D,Ghiaur G,Dunn SK,Ficker AM,Murali B,Madhu M,Gutstein DE,Fishman GI,Barrio LC,Cancelas JA

Tue Jun 05 00:00:00 EDT 2012

Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms.

Proceedings of the National Academy of Sciences of the United States of America

Guo S,Bai H,Megyola CM,Halene S,Krause DS,Scadden DT,Lu J

Tue Oct 09 00:00:00 EDT 2012

Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBFβ interaction.

Proceedings of the National Academy of Sciences of the United States of America

Cunningham L,Finckbeiner S,Hyde RK,Southall N,Marugan J,Yedavalli VR,Dehdashti SJ,Reinhold WC,Alemu L,Zhao L,Yeh JR,Sood R,Pommier Y,Austin CP,Jeang KT,Zheng W,Liu P

Tue Sep 04 00:00:00 EDT 2012

NLRP3 inflammasome activity is negatively controlled by miR-223.

Journal of immunology (Baltimore, Md. : 1950)

Bauernfeind F,Rieger A,Schildberg FA,Knolle PA,Schmid-Burgk JL,Hornung V

Mon Oct 15 00:00:00 EDT 2012

TAK1 (MAP3K7) signaling regulates hematopoietic stem cells through TNF-dependent and -independent mechanisms.

PloS one

Takaesu G,Inagaki M,Takubo K,Mishina Y,Hess PR,Dean GA,Yoshimura A,Matsumoto K,Suda T,Ninomiya-Tsuji J

Tue May 28 00:00:00 EDT 2013

Induction of histiocytic sarcoma in mouse skeletal muscle.

PloS one

Liu J,Hettmer S,Milsom MD,Hofmann I,Hua F,Miller C,Bronson RT,Wagers AJ

Thu Feb 07 00:00:00 EST 2013

The TspanC8 subgroup of tetraspanins interacts with A disintegrin and metalloprotease 10 (ADAM10) and regulates its maturation and cell surface expression.

The Journal of biological chemistry

Haining EJ,Yang J,Bailey RL,Khan K,Collier R,Tsai S,Watson SP,Frampton J,Garcia P,Tomlinson MG

Fri Nov 16 00:00:00 EST 2012

Rho kinase regulates the survival and transformation of cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL.

Cancer cell

Mali RS,Ramdas B,Ma P,Shi J,Munugalavadla V,Sims E,Wei L,Vemula S,Nabinger SC,Goodwin CB,Chan RJ,Traina F,Visconte V,Tiu RV,Lewis TA,Stern AM,Wen Q,Crispino JD,Boswell HS,Kapur R

Tue Sep 13 00:00:00 EDT 2011

SHP2 tyrosine phosphatase stimulates CEBPA gene expression to mediate cytokine-dependent granulopoiesis.

Blood

Zhang L,Friedman AD

Thu Aug 25 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; ML; Myeloproliferative leukemia virus oncogene ligand; Thrombopoietin

Mouse TPO (Thrombopoietin), Animal-Free Recombinant Protein, PeproTech®

Mouse TPO (Thrombopoietin), Animal-Free Protein (AF-315-14-10UG) in Functional

Product Details

AF-315-14-10UG

Immunohistochemistry (IHC)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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