Gibco

Rat CCL2 (MCP-1) Recombinant Protein, PeproTech®

Product Details

400-12-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 6 publications 6 publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Rat

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN

Molecular weight

14 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

400-12-1MG will be provided as 2 x 500 µg (400-12-500UG).

Recombinant Rat MCP-1 is a 14.0 kDa protein containing 125 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization.

World journal of stem cells

Yang HC,Zhang M,Wu R,Zheng HQ,Zhang LY,Luo J,Li LL,Hu XQ

400-12 was used in Enzyme-linked immunosorbent assay to find that ExoCCR2 could suppress the CCL2-induced macrophage migration and activation in vivo and in vitro, compared with ExoCtrl treated group.

Wed Feb 26 00:00:00 EST 2020

Alveolar Epithelial Cell-Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Speth JM,Bourdonnay E,Penke LR,Mancuso P,Moore BB,Weinberg JB,Peters-Golden M

Wed Jun 15 00:00:00 EDT 2016

Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Szmydynger-Chodobska J,Strazielle N,Gandy JR,Keefe TH,Zink BJ,Ghersi-Egea JF,Chodobski A

Sun Jan 01 00:00:00 EST 2012

CCL2/MCP-1 modulation of microglial activation and proliferation.

Journal of neuroinflammation

Hinojosa AE,Garcia-Bueno B,Leza JC,Madrigal JL

Tue Jul 05 00:00:00 EDT 2011

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.

Journal of immunology (Baltimore, Md. : 1950)

Abu-Taha M,Rius C,Hermenegildo C,Noguera I,Cerda-Nicolas JM,Issekutz AC,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Wed Jul 15 00:00:00 EDT 2009

A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.

Blood

Mateo T,Naim Abu Nabah Y,Losada M,Estellés R,Company C,Bedrina B,Cerdá-Nicolás JM,Poole S,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Sat Sep 15 00:00:00 EDT 2007

CXCR2 blockade impairs angiotensin II-induced CC chemokine synthesis and mononuclear leukocyte infiltration.

Arteriosclerosis, thrombosis, and vascular biology

Abu Nabah YN,Losada M,Estellés R,Mateo T,Company C,Piqueras L,Lopez-Gines C,Sarau H,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

Thu Nov 01 00:00:00 EDT 2007

CXCR2 blockade impairs angiotensin II-induced CC chemokine synthesis and mononuclear leukocyte infiltration.

Arteriosclerosis, thrombosis, and vascular biology

Abu Nabah YN,Losada M,Estellés R,Mateo T,Company C,Piqueras L,Lopez-Gines C,Sarau H,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

Thu Nov 01 00:00:00 EDT 2007

Purinergic P2Y2 receptors induce increased MCP-1/CCL2 synthesis and release from rat alveolar and peritoneal macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Stokes L,Surprenant A

Thu Nov 01 00:00:00 EDT 2007

Monocyte chemotactic protein-1 regulates leukocyte recruitment during gastric ulcer recurrence induced by tumor necrosis factor-alpha.

American journal of physiology. Gastrointestinal and liver physiology

Watanabe T,Higuchi K,Hamaguchi M,Shiba M,Tominaga K,Fujiwara Y,Matsumoto T,Arakawa T

Fri Oct 01 00:00:00 EDT 2004

Beneficial autoimmunity to proinflammatory mediators restrains the consequences of self-destructive immunity.

Immunity

Wildbaum G,Nahir MA,Karin N

Sat Nov 01 00:00:00 EST 2003

Liver-infiltrating T lymphocytes are attracted selectively by IFN-inducible protein-10.

Cytokine

Tamaru M,Nishioji K,Kobayashi Y,Watanabe Y,Itoh Y,Okanoue T,Murai M,Matsushima K,Narumi S

Sat Apr 01 00:00:00 EST 2000

Chronic inflammation upregulates chemokine receptors and induces neutrophil migration to monocyte chemoattractant protein-1.

The Journal of clinical investigation

Johnston B,Burns AR,Suematsu M,Issekutz TB,Woodman RC,Kubes P

Sat May 01 00:00:00 EDT 1999

Angiotensin II induces monocyte chemoattractant protein-1 gene expression in rat vascular smooth muscle cells.

Circulation research

Chen XL,Tummala PE,Olbrych MT,Alexander RW,Medford RM

Mon Nov 02 00:00:00 EST 1998

In vivo inhibition of CC and CX3C chemokine-induced leukocyte infiltration and attenuation of glomerulonephritis in Wistar-Kyoto (WKY) rats by vMIP-II.

The Journal of experimental medicine

Chen S,Bacon KB,Li L,Garcia GE,Xia Y,Lo D,Thompson DA,Siani MA,Yamamoto T,Harrison JK,Feng L

Mon Jul 06 00:00:00 EDT 1998

Dendritic cells are recruited into the airway epithelium during the inflammatory response to a broad spectrum of stimuli.

The Journal of experimental medicine

McWilliam AS,Napoli S,Marsh AM,Pemper FL,Nelson DJ,Pimm CL,Stumbles PA,Wells TN,Holt PG

Sun Dec 01 00:00:00 EST 1996

Bioinformatics

Protein Aliases: C-C motif chemokine 2; H-MCP-1; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; RP23-350G1.3; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2

Rat CCL2 (MCP-1) Recombinant Protein, PeproTech®

Product Details

400-12-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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