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Product Details
PRC1054
Applications
Tested Dilution
Publications
Product Specifications
Species
Rat
Expression System
E. coli
Amino acid sequence
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
Molecular weight
7.9 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 10 - 100 ng/mL; determined by the dose-dependent chemotaxis of human blood monocytes.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Recombinant rat RANTES should be reconstituted in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in tissue culture grade buffered solutions with heat inactivated fetal calf serum or low endotoxin BSA.
Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.
Target Information
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 5; MGC17164; OTTHUMP00000197106; regulated upon activation normal T-cell expressed and secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES
Gene Aliases: Rantes; Scya5
Entrez Gene ID: (Rat) 81780
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