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Product Details
131001
Applications
Tested Dilution
Publications
Product Specifications
Species
Rat
Expression System
E. coli
Molecular weight
19 kDa
Class
Recombinant
Type
Protein
Purity
>95%
Endotoxin concentration
<1 EU/µg
Activity
EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]As there is no international Rat IFN-alpha standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha/beta) provided by the National Institutes of Health.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
citric acid, pH 5, with NaCl, 0.125M trehalose
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Each blood donation used for interferon production tested negative for: HBsAg, HIV I-II, HCV and VDRL.
After receipt, this product should be kept at 4ºC while lyophilized and at -70ºC after reconstitution in sterile distilled water for retention of full activity. After reconstitution store at -70°C in aliquots. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Further dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IFN-a; IFN-alpha 1b; IFNA 2; interferon alpha 1b; interferon alpha family, gene 2; interferon alpha-D; Interferon-alpha II; leIF D; RP11-354P17.2
Entrez Gene ID: (Rat) 298213
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