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Invitrogen
SARS-CoV-2 Spike Protein (RBD) (N501Y) Fc Chimera Recombinant Protein
Product Details
RP-88315
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
SARS-CoV-2 spike RBD (Accession # YP_009724390.1), amino acids Arg319-Phe541 with an N501Y substitution and a C-terminal rabbit IgG1 Fc region
Tag
Fc-tag
Molecular weight
52.5 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus.
The mutation was identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
The mutations were identified in the SARS-CoV-2 varient known as 20B/501Y.V1 or VOC 202012/01 or B.1.1.7
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
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