Invitrogen

CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), FITC, eBioscience™

9 Published Figures

25 References

View all (223) CD11b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

CD11b (activation epitope) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD11b (activation epitope) Antibody (11-0113-42) in Flow

Staining of 5-minute PMA/Ionomycin-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD11b (activation epitope) FITC (purple histogram). The orange histogram depicts staining of unstimulated cells with the Anti-Human CD11b (activation epitope) FITC antibody. Cells in the granulocyte gate were used for analysis.

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Product Details

11-0113-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

- -

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CBRM1/5

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572437

Product Specific Information

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.

Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Targeting formyl peptide receptor 1 with anteiso-C13-surfactin for neutrophil-dominant acute respiratory distress syndrome.

British journal of pharmacology

Yang SC,Wang YH,Ho CM,Tsai YF,Sung PJ,Lin TE,Hwang TL

11-0113-42 was used in Flow Cytometry to identify IA-1 as a competitive FPR1 antagonist, thus diminishing the downstream signalling pathways involving calcium, mitogen-activated protein kinases and Akt.

Tue Aug 01 00:00:00 EDT 2023

Lipoxins modulate neutrophil oxidative burst, integrin expression and lymphatic transmigration differentially in human health and atherosclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kraft JD,Blomgran R,Bergström I,Soták M,Clark M,Rani A,Rajan MR,Dalli J,Nyström S,Quiding-Järbrink M,Bromberg J,Skoog P,Börgeson E

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Upregulation of CD3ζ and L-selectin in antigen-specific cytotoxic T lymphocytes by eliminating myeloid-derived suppressor cells with doxorubicin to improve killing efficacy of neuroblastoma cells in vitro.

Journal of clinical laboratory analysis

Xu W,Li S,Li M,Zhou H,Yang X

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

RAS-Responsive Element-Binding Protein 1 Blocks the Granulocytic Differentiation of Myeloid Leukemia Cells.

Oncology research

Yao J,Zhong L,Zhong P,Liu D,Yuan Z,Liu J,Yao S,Zhao Y,Chen M,Li L,Liu L,Liu B

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Fri Jul 12 00:00:00 EDT 2019

Deficiency of FAM3D (Family With Sequence Similarity 3, Member D), A Novel Chemokine, Attenuates Neutrophil Recruitment and Ameliorates Abdominal Aortic Aneurysm Development.

Arteriosclerosis, thrombosis, and vascular biology

He L,Fu Y,Deng J,Shen Y,Wang Y,Yu F,Xie N,Chen Z,Hong T,Peng X,Li Q,Zhou J,Han J,Wang Y,Xi J,Kong W

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte‑associated immunoglobulin‑like receptor 1.

Molecular medicine reports

Fu Q,Man X,Yu M,Chu Y,Luan X,Piao H,Xue J,Jin C

11-0113-42 was used in Flow cytometry/Cell sorting to identify mesenchymal stem cells (MSCs) from human first-trimester deciduas, termed decidual MSCs (DMSCs), and to investigate the effect of DMSCs on the regulation of dNK cells via collagen.

Fri Sep 01 00:00:00 EDT 2017

TLR2 activation induces antioxidant defence in human monocyte-macrophage cell line models.

Oncotarget

Karwaciak I,Gorzkiewicz M,Bartosz G,Pulaski L

11-0113 was used in Flow cytometry/Cell sorting to determine whether there are specific biochemical mechanisms responsible for an increase in oxidative stress resistance in differentiating macrophages.

Tue Aug 15 00:00:00 EDT 2017

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

NLS‑RARα modulates acute promyelocytic leukemia NB4 cell proliferation and differentiation via the PI3K/AKT pathway.

Molecular medicine reports

Song H,Li L,Zhong L,Yang R,Jiang K,Yang X,Liu B

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Differential in vivo activation of monocyte subsets during low-grade inflammation through experimental endotoxemia in humans.

Scientific reports

Thaler B,Hohensinner PJ,Krychtiuk KA,Matzneller P,Koller L,Brekalo M,Maurer G,Huber K,Zeitlinger M,Jilma B,Wojta J,Speidl WS

11-0113 was used in Flow cytometry/Cell sorting to explore the effects of low-grade inflammation on the activation and distribution of different monocyte subsets.

Fri Jul 22 00:00:00 EDT 2016

Histidine-Rich Glycoprotein Prevents Septic Lethality through Regulation of Immunothrombosis and Inflammation.

EBioMedicine

Wake H,Mori S,Liu K,Morioka Y,Teshigawara K,Sakaguchi M,Kuroda K,Gao Y,Takahashi H,Ohtsuka A,Yoshino T,Morimatsu H,Nishibori M

11-0113 was used in Flow cytometry/Cell sorting to suggest that supplementary therapy with HRG can help to treat sepsis through suppression of excessive systemic inflammation and immunothrombosis.

Fri Jul 01 00:00:00 EDT 2016

A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells.

Oncotarget

Song G,Shi L,Guo Y,Yu L,Wang L,Zhang X,Li L,Han Y,Ren X,Guo Q,Bi K,Jiang G

11-0113 was used in Flow cytometry/Cell sorting to highlight an association between PAD4 and DNA hypermethylation in APL and demonstrate that targeting PAD4 or regulating its downstream effectors may be a promising strategy to control differentiation.

Tue Jan 19 00:00:00 EST 2016

Science China. Life sciences

Zeng C,Wang W,Yu X,Yang L,Chen S,Li Y

11-0113 was used in Flow cytometry/Cell sorting to provide numerous potential therapeutic targets for modulation of the differentiation of monocytic leukemia.

Tue Dec 01 00:00:00 EST 2015

Serum biomarkers VEGF-C and IL-6 are associated with severe human Peripheral Artery Stenosis.

Journal of inflammation (London, England)

Chen J,Han L,Xu X,Tang H,Wang H,Wei B

11-0113 was used in Immunofluorescence to investigate which biomarkers in serum are associated with the severity of PAS.

Tue Aug 18 00:00:00 EDT 2015

Serum biomarkers VEGF-C and IL-6 are associated with severe human Peripheral Artery Stenosis.

Journal of inflammation (London, England)

Chen J,Han L,Xu X,Tang H,Wang H,Wei B

11-0113 was used in Immunofluorescence to investigate which biomarkers in serum are associated with the severity of PAS.

Tue Aug 18 00:00:00 EDT 2015

MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia.

Autophagy

Zeng CW,Chen ZH,Zhang XJ,Han BW,Lin KY,Li XJ,Wei PP,Zhang H,Li Y,Chen YQ

11-0113 was used in Flow cytometry/Cell sorting to demonstrate MIR125B1 dysregulation may interfere with ATRA-mediated differentiation through an autophagy-dependent pathway.

Wed Oct 01 00:00:00 EDT 2014

Fluid shear stress increases neutrophil activation via platelet-activating factor.

Biophysical journal

Mitchell MJ,Lin KS,King MR

11-0113 was used in Flow cytometry/Cell sorting to demonstrate that fluid shear stress exposure differentially controls how neutrophils respond to chemoattractants.

Tue May 20 00:00:00 EDT 2014

c-myc but not Hif-1α-dependent downregulation of VEGF influences the proliferation and differentiation of HL-60 cells induced by ATRA.

Oncology reports

Song G,Li Y,Zhang Z,Ren X,Li H,Zhang W,Wei R,Pan S,Shi L,Bi K,Jiang G

11-0113-42 was used in Flow Cytometry to investigate the role of c-myc in modulating the expression of vascular endothelial growth factor in HL-60 cells.

Sat Jun 01 00:00:00 EDT 2013

Adiponectin inhibits neutrophil phagocytosis of Escherichia coli by inhibition of PKB and ERK 1/2 MAPK signalling and Mac-1 activation.

PloS one

Rossi A,Lord J

11-0113 was used in Flow cytometry/Cell sorting to investigate the role of adiponectin in neutrophils.

Wed Mar 05 00:00:00 EST 2014

Gu-4 suppresses affinity and avidity modulation of CD11b and improves the outcome of mice with endotoxemia and sepsis.

PloS one

Yan T,Li Q,Zhou H,Zhao Y,Yu S,Xu G,Yin Z,Li Z,Zhao Z

Published figure using CD11b (activation epitope) monoclonal antibody (Product # 11-0113-42) in Flow Cytometry

Mon Sep 24 00:00:00 EDT 2012

Common genetic variations in the NALP3 inflammasome are associated with delayed apoptosis of human neutrophils.

PloS one

Blomgran R,Patcha Brodin V,Verma D,Bergström I,Söderkvist P,Sjöwall C,Eriksson P,Lerm M,Stendahl O,Särndahl E

11-0113 was used in Flow cytometry/Cell sorting to investigate whether the inflammatory phenotype caused by the Q705K/C10X NALP3 polymorphism was related to dysfunctional apoptosis.

Mon Jul 09 00:00:00 EDT 2012

Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.

Journal of leukocyte biology

Feng C,Zhang L,Almulki L,Faez S,Whitford M,Hafezi-Moghadam A,Cross AS

11-0113 was used in Flow cytometry/Cell sorting to investigate how sialylation of adhesion molecules expressed on endothelial cells and polymorphonuclear cells affects the adhesion between these cells.

Mon Aug 01 00:00:00 EDT 2011

Outside-in signal transmission by conformational changes in integrin Mac-1.

Journal of immunology (Baltimore, Md. : 1950)

Lefort CT,Hyun YM,Schultz JB,Law FY,Waugh RE,Knauf PA,Kim M

11-0113 was used in Flow cytometry/Cell sorting to investigate how integrin Mac-1 structure mediates signal transduction from outside to inside the cell.

Sun Nov 15 00:00:00 EST 2009

CD44-mediated phagocytosis induces inside-out activation of complement receptor-3 in murine macrophages.

Blood

Vachon E,Martin R,Kwok V,Cherepanov V,Chow CW,Doerschuk CM,Plumb J,Grinstein S,Downey GP

11-0113 was used in Flow cytometry/Cell sorting to suggest that CD44 stimulation induces inside-out activation of CR3 through the GTPase Rap1.

Sat Dec 15 00:00:00 EST 2007

Antineutrophil cytoplasm antibody-stimulated neutrophil adhesion depends on diacylglycerol kinase-catalyzed phosphatidic acid formation.

Journal of the American Society of Nephrology : JASN

Williams JM,Pettitt TR,Powell W,Grove J,Savage CO,Wakelam MJ

11-0113 was used in Flow cytometry/Cell sorting to investigate the signalling pathways activated by circulating antineutrophil cytoplasmic antibodies in systematic vasculitis to identify novel drug targets.

Sun Apr 01 00:00:00 EDT 2007

ANCA induces beta2 integrin and CXC chemokine-dependent neutrophil-endothelial cell interactions that mimic those of highly cytokine-activated endothelium.

Journal of leukocyte biology

Calderwood JW,Williams JM,Morgan MD,Nash GB,Savage CO

11-0113 was used in Flow cytometry/Cell sorting to investigate the role of beta2 integrins and chemokine receptors in neutrophil migration through the endothelium.

Sat Jan 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Human) 3684

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Function(s)

glycoprotein binding protein binding metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration

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CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), FITC, eBioscience™

CD11b (activation epitope) Antibody (11-0113-42) in Flow

Product Details

11-0113-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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