Invitrogen

CD40 Monoclonal Antibody (5C3), FITC, eBioscience™

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CD40 Antibody (11-0409-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0409-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5C3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272068

Product Specific Information

Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Applications Reported: The 5C3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 5C3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

11-0409-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 EDT 2021

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

11-0409-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 EDT 2021

Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis.

Immunology and cell biology

Carstensen M,Christensen T,Stilund M,Møller HJ,Petersen EL,Petersen T

Published figure using CD40 monoclonal antibody (Product # 11-0409-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD40 monoclonal antibody (Product # 11-0409-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells.

Frontiers in immunology

Richardson JR,Armbruster NS,Günter M,Henes J,Autenrieth SE

11-0409-42 was used in Flow Cytometry to study the effect of PSMα3 on the maturation, cytokine production, antigen uptake, and T cell stimulatory capacity of human monocyte-derived DCs treated simultaneously with either LPS(TLR4 ligand) or Sa cell lysate (TLR2 ligand).

Thu Oct 03 00:00:00 EDT 2019

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using CD40 monoclonal antibody (Product # 11-0409-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application.

Scientific reports

Mihajlovic M,van den Heuvel LP,Hoenderop JG,Jansen J,Wilmer MJ,Westheim AJF,Allebes WA,Stamatialis D,Hilbrands LB,Masereeuw R

Wed Aug 02 00:00:00 EDT 2017

Activated macrophages control human adipocyte mitochondrial bioenergetics via secreted factors.

Molecular metabolism

Keuper M,Sachs S,Walheim E,Berti L,Raedle B,Tews D,Fischer-Posovszky P,Wabitsch M,Hrabě de Angelis M,Kastenmüller G,Tschöp MH,Jastroch M,Staiger H,Hofmann SM

11-0409 was used in Flow cytometry/Cell sorting to delineate how human macrophages may affect the energy metabolism of white adipocytes.

Sun Oct 01 00:00:00 EDT 2017

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD40 monoclonal antibody (Product # 11-0409-42) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.

Journal of immunology (Baltimore, Md. : 1950)

Joo H,Upchurch K,Zhang W,Ni L,Li D,Xue Y,Li XH,Hori T,Zurawski S,Liu YJ,Zurawski G,Oh S

11-0409 was used in Flow cytometry/Cell sorting to investigate the role of Dectin-1 in dendritic cells, showing opposing roles between plasmacytoid and myeloid dendritic cells in Th2 polarisation.

Sat Aug 15 00:00:00 EDT 2015

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

Published figure using CD40 monoclonal antibody (Product # 11-0409-42) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.

Blood

Ozelo MC,Vidal B,Brown C,Notley C,Hegadorn C,Webster S,Harpell L,Ahlin J,Winterborn A,Handforth J,Arruda VR,Hough C,Lillicrap D

11-0409 was used in Flow cytometry/Cell sorting to investigate the immune response to treatment with FVIII-expressing autologous BOECs.

Thu Jun 26 00:00:00 EDT 2014

Identification of B cells through negative gating-An example of the MIFlowCyt standard applied.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Blimkie D,Fortuno ES,Thommai F,Xu L,Fernandes E,Crabtree J,Rein-Weston A,Jansen K,Brinkman RR,Kollmann TR

11-0409 was used in Flow cytometry/Cell sorting to demonstrate how a MIFlowCyt-compliant report can be prepared with minimal effort, and yet provide the reader with a much clearer picture of the portrayed FCM experiment and data.

Tue Jun 01 00:00:00 EDT 2010

Polychromatic flow cytometric high-throughput assay to analyze the innate immune response to Toll-like receptor stimulation.

Journal of immunological methods

Jansen K,Blimkie D,Furlong J,Hajjar A,Rein-Weston A,Crabtree J,Reikie B,Wilson C,Kollmann T

11-0409 was used in Flow cytometry/Cell sorting to describe a novel platform that enables the interrogation and screening of APC responses to TLR ligands.

Thu Jul 31 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5

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Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5

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UniProt ID: (Human) P25942

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Entrez Gene ID: (Human) 958

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Function(s)

antigen binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (5C3), FITC, eBioscience™

CD40 Antibody (11-0409-42) in Flow

Product Details

11-0409-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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