Invitrogen

CD41a Monoclonal Antibody (HIP8), FITC, eBioscience™

4 Published Figures

18 References

View all (83) CD41 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD41a Antibody (11-0419-42) in Flow

Staining of human platelets with Anti-Human CD42b APC and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD41a FITC (right). Total events were used for analysis.

CD41a Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

11-0419-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 15 publications 15 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIP8

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718234

Product Specific Information

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alphaIIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation.

Applications Reported: The HIP8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia.

Nature immunology

Voit RA,Tao L,Yu F,Cato LD,Cohen B,Fleming TJ,Antoszewski M,Liao X,Fiorini C,Nandakumar SK,Wahlster L,Teichert K,Regev A,Sankaran VG

11-0419-42 was used in Flow cytometry/Cell sorting to show that this transcriptional network is co-opted in high-risk leukemias, thereby enabling these cancers to acquire stem cell properties.

Sun Jan 01 00:00:00 EST 2023

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD41a monoclonal antibody (Product # 11-0419-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.

Platelets

Butov KR,Osipova EY,Mikhalkin NB,Trubina NM,Panteleev MA,Machlus KR

11-0419-42 was used in Flow cytometry/Cell sorting to propose a simple, robust, and adapted method of primary human MK culture from 1 mL of bone marrow aspirate.

Sun Oct 03 00:00:00 EDT 2021

Resolving the immune landscape of human prostate at a single-cell level in health and cancer.

Cell reports

Tuong ZK,Loudon KW,Berry B,Richoz N,Jones J,Tan X,Nguyen Q,George A,Hori S,Field S,Lynch AG,Kania K,Coupland P,Babbage A,Grenfell R,Barrett T,Warren AY,Gnanapragasam V,Massie C,Clatworthy MR

11-0419-42 was used in Flow cytometry/Cell sorting to identify a subset of tumor-enriched androgen receptor-negative luminal epithelial cells with increased expression of cancer-associated genes.

Tue Dec 21 00:00:00 EST 2021

Yersinia pestis escapes entrapment in thrombi by targeting platelet function.

Journal of thrombosis and haemostasis : JTH

Palace SG,Vitseva O,Proulx MK,Freedman JE,Goguen JD,Koupenova M

11-0419-42 was used in Immunomicroscopy to determine whether extracellular bloodborne bacterial pathogens actively interfere with platelet function, using Y pestis as a model system.

Tue Dec 01 00:00:00 EST 2020

SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients.

Research square

Sahai A,Bhandari R,Koupenova M,Freedman JE,Godwin M,McIntyre T,Chung MK,Iskandar JP,Kamran H,Hariri E,Aggarwal A,Kalra A,Bartholomew JR,McCrae KR,Elbadawi A,Svensson LG,Kapadia S,Cameron SJ

11-0419-42 was used in Immunocytochemistry to suggest that human platelets express ACE2-TMPRSS2 receptor-protease axis for SARS-CoV-2 infection, however aspirin does not prevent thrombosis and death in COVID-19.

Wed Dec 23 00:00:00 EST 2020

SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients.

Research square

Sahai A,Bhandari R,Koupenova M,Freedman JE,Godwin M,McIntyre T,Chung MK,Iskandar JP,Kamran H,Hariri E,Aggarwal A,Kalra A,Bartholomew JR,McCrae KR,Elbadawi A,Svensson LG,Kapadia S,Cameron SJ

Wed Dec 23 00:00:00 EST 2020

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

11-0419 was used in Flow cytometry/Cell sorting to show peripheral regulatory T cells can be induced by platelets through GARP and could explain why poor prognosis and metastasis are associated with high counts of circulating platelets.

Sat Dec 05 00:00:00 EST 2020

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Sat Dec 05 00:00:00 EST 2020

Platelets Fuel the Inflammasome Activation of Innate Immune Cells.

Cell reports

Rolfes V,Ribeiro LS,Hawwari I,Böttcher L,Rosero N,Maasewerd S,Santos MLS,Próchnicki T,Silva CMS,Wanderley CWS,Rothe M,Schmidt SV,Stunden HJ,Bertheloot D,Rivas MN,Fontes CJ,Carvalho LH,Cunha FQ,Latz E,Arditi M,Franklin BS

11-0419-42 was used in Flow Cytometry to conclude that platelets provide an additional layer of regulation of inflammasomes and IL-1-driven inflammation.

Tue May 12 00:00:00 EDT 2020

T cell-derived soluble glycoprotein GPIbα mediates PGE₂ production in human monocytes activated with the vaccine adjuvant MDP.

Science signaling

Liu F,Endo Y,Romantseva T,Wu WW,Akue A,Shen RF,Golding H,Zaitseva M

11-0419 was used in Flow cytometry/Cell sorting to study the mechanism of prostaglandin E2 production by human monocytes activated with muramyl dipeptide (MDP) adjuvant, which activates cytosolic nucleotide-binding oligomerization domain 2 (NOD2).

Tue Oct 08 00:00:00 EDT 2019

Mitochondrial DNA in the tumour microenvironment activates neutrophils and is associated with worse outcomes in patients with advanced epithelial ovarian cancer.

British journal of cancer

Singel KL,Grzankowski KS,Khan ANMNH,Grimm MJ,D'Auria AC,Morrell K,Eng KH,Hylander B,Mayor PC,Emmons TR,Lénárt N,Fekete R,Környei Z,Muthukrishnan U,Gilthorpe JD,Urban CF,Itagaki K,Hauser CJ,Leifer C,Moysich KB,Odunsi K,Dénes Á,Segal BH

Published figure using CD41a monoclonal antibody (Product # 11-0419-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

The role of platelets in mediating a response to human influenza infection.

Nature communications

Koupenova M,Corkrey HA,Vitseva O,Manni G,Pang CJ,Clancy L,Yao C,Rade J,Levy D,Wang JP,Finberg RW,Kurt-Jones EA,Freedman JE

11-0419 was used in Immunocytochemistry-immunoflourescence to determine if platelet processes are activated during influenza infection.

Tue Apr 16 00:00:00 EDT 2019

Pollen-derived RNAs Are Found in the Human Circulation.

iScience

Koupenova M,Mick E,Corkrey HA,Singh A,Tanriverdi SE,Vitseva O,Levy D,Keeler AM,Ezzaty Mirhashemi M,ElMallah MK,Gerstein M,Rozowsky J,Tanriverdi K,Freedman JE

11-0419 was used in Flow cytometry to demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans.

Fri Sep 27 00:00:00 EDT 2019

PD-L1 is expressed on human platelets and is affected by immune checkpoint therapy.

Oncotarget

Rolfes V,Idel C,Pries R,Plötze-Martin K,Habermann J,Gemoll T,Bohnet S,Latz E,Ribbat-Idel J,Franklin BS,Wollenberg B

11-0419 was used in Flow cytometry/Cell sorting to demonstrate the expression and upregulation of PD-L1 on platelets and peripheral blood mononuclear cells from smokers and patients with head and neck squamous cell carcinoma or lung cancer.

Fri Jun 08 00:00:00 EDT 2018

The Plant Hormone Abscisic Acid Is a Prosurvival Factor in Human and Murine Megakaryocytes.

The Journal of biological chemistry

Malara A,Fresia C,Di Buduo CA,Soprano PM,Moccia F,Balduini C,Zocchi E,De Flora A,Balduini A

11-0419 was used in Flow cytometry/Cell sorting to study the role of exogenous abscisic acid in modulating thrombopoiesis.

Fri Feb 24 00:00:00 EST 2017

Circulating microparticles carry oxidation-specific epitopes and are recognized by natural IgM antibodies.

Journal of lipid research

Tsiantoulas D,Perkmann T,Afonyushkin T,Mangold A,Prohaska TA,Papac-Milicevic N,Millischer V,Bartel C,Hörkkö S,Boulanger CM,Tsimikas S,Fischer MB,Witztum JL,Lang IM,Binder CJ

11-0419 was used in Flow cytometry/Cell sorting to demonstrate a novel mechanism by which anti-OxLDL IgM antibodies could mediate protective functions in CVD.

Sun Feb 01 00:00:00 EST 2015

Bone marrow stromal cells transduced with a thrombopoietin, interleukin-6, and interleukin-11 syncretic gene induce cord mononuclear cells to generate platelets in vitro.

Transfusion

Lu H,Jiang T,Li R,Wang S,Zhang Q,Zhao S

11-0419 was used in Flow cytometry/Cell sorting to suggest that our method could be used to develop a low-cost sustainable cultivation system that generates PLTs in vitro by enhancing the autocrine production of related cytokines.

Thu Jan 01 00:00:00 EST 2015

Association between toll-like receptor 6 expression and auxiliary T cells in the peripheral blood of pediatric patients with allergic purpura.

Experimental and therapeutic medicine

Chang H,Cao Y,Lin YI,Zhu H,Fu Y,Chen X,Zhang Q

11-0419 was used in Flow cytometry/Cell sorting to investigate the correlations between Toll-like receptor 6 expression in the peripheral blood mononuclear cells and auxiliary T cells of children with purpura.

Thu Oct 01 00:00:00 EDT 2015

Constitutively released adenosine diphosphate regulates proplatelet formation by human megakaryocytes.

Haematologica

Balduini A,Di Buduo CA,Malara A,Lecchi A,Rebuzzini P,Currao M,Pallotta I,Jakubowski JA,Cattaneo M

11-0419 was used in Flow cytometry/Cell sorting to study the role of adenosine diphosphate and P2Y receptors on proplatelet formation by human megakaryocytes in culture.

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alphaIIb protein; CD41; form 1; form 2; GPalpha IIb; GPIIb; Integrin alpha-IIb; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); platelet fibrinogen receptor, alpha subunit; platelet glycoprotein IIb of IIb/IIIa complex; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK; protein phosphatase 1, regulatory subunit 93

View more View less

Gene Aliases: BDPLT16; BDPLT2; CD41; CD41B; GP2B; GPIIb; GT; GTA; HPA3; ITGA2B; ITGAB; PPP1R93

View more View less

UniProt ID: (Human) P08514

View more View less

Entrez Gene ID: (Human) 3674

View more View less

Function(s)

protein binding identical protein binding metal ion binding extracellular matrix binding fibrinogen binding integrin

Process(es)

platelet degranulation positive regulation of leukocyte migration cell adhesion cell-matrix adhesion integrin-mediated signaling pathway extracellular matrix organization platelet aggregation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD41a Monoclonal Antibody (HIP8), FITC, eBioscience™

CD41a Antibody (11-0419-42) in Flow

Product Details

11-0419-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!