Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), FITC, eBioscience™

7 Published Figures

9 References

View all (70) CD49b (Integrin alpha 2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD49b (Integrin alpha 2) Antibody (11-0491-82) in Flow

Staining of mouse splenocytes with 0.06 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (open histogram) or 0.06 µg of Anti-Mouse CD49b (Integrin alpha 2) FITC (filled histogram). Total viable cells were used for analysis.

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Product Details

11-0491-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HMa2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465080

Product Specific Information

Description: The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells.

Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Platelet Dysfunction and Thrombosis in JAK2^V617F-Mutated Primary Myelofibrotic Mice.

Arteriosclerosis, thrombosis, and vascular biology

Matsuura S,Thompson CR,Belghasem ME,Bekendam RH,Piasecki A,Leiva O,Ray A,Italiano J,Yang M,Merill-Skoloff G,Chitalia VC,Flaumenhaft R,Ravid K

11-0491 was used in Flow cytometry/Cell sorting to examine the propensity for thrombosis, as well as platelet activation properties in a mouse model of primary myelofibrosis induced by JAK2V617F mutation.

Thu Oct 01 00:00:00 EDT 2020

N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells.

Cell reports

Zhao M,Tao F,Venkatraman A,Li Z,Smith SE,Unruh J,Chen S,Ward C,Qian P,Perry JM,Marshall H,Wang J,He XC,Li L

11-0491 was used in Flow cytometry/Cell sorting to report that N-cad+ cells were functional bone and marrow stromal progenitor cells, giving rise to osteoblasts, adipocytes, and chondrocytes both in vitro and in vivo.

Tue Jan 15 00:00:00 EST 2019

Cytochrome P450 26A1 modulates natural killer cells in mouse early pregnancy.

Journal of cellular and molecular medicine

Meng CY,Li ZY,Fang WN,Song ZH,Yang DD,Li DD,Yang Y,Peng JP

11-0491 was used in Flow cytometry/Cell sorting to provide evidence correlating the deleterious effects of blocking CYP26A1 in pregnancy with the aberrant regulation of NK cells in the uterus.

Sat Apr 01 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

11-0491 was used in Flow cytometry/Cell sorting to investigate the developmental stage of tumour-associated NK cells, showing that the tumour microenvironment alters the phenotype and function of NK cells.

Thu Sep 01 00:00:00 EDT 2016

Multicomponent LBSap vaccine displays immunological and parasitological profiles similar to those of Leish-Tec® and Leishmune® vaccines against visceral leishmaniasis.

Parasites & vectors

de Mendonça LZ,Resende LA,Lanna MF,Aguiar-Soares RD,Roatt BM,Castro RA,Batista MA,Silveira-Lemos D,Gomes Jde A,Fujiwara RT,Rezende SA,Martins-Filho OA,Corrêa-Oliveira R,Dutra WO,Reis AB,Giunchetti RC

11-0491 was used in Flow cytometry/Cell sorting to compare the effects of the multicomponent LBSap vaccine with those of Leish-Tec® and Leishmune®.

Tue Aug 30 00:00:00 EDT 2016

Decrease of peripheral and intestinal NKG2A-positive T cells in patients with ulcerative colitis.

PloS one

Katsurada T,Kobayashi W,Tomaru U,Baba T,Furukawa S,Ishizu A,Takeda K,Sakamoto N,Asaka M,Takeda H,Kasahara M

11-0491 was used in Flow cytometry/Cell sorting to analyse the expression of the inhibitory natural killer receptor NKG2A on T cells in a mouse colitis model and human inflammatory bowel disease.

Wed May 01 00:00:00 EDT 2013

Macrophages help NK cells to attack tumor cells by stimulatory NKG2D ligand but protect themselves from NK killing by inhibitory ligand Qa-1.

PloS one

Zhou Z,Zhang C,Zhang J,Tian Z

11-0491 was used in Flow cytometry/Cell sorting to examine the role of the interaction between natural killer cells and macrophages in the regulation of the anti-tumour activities of natural killer cells.

Mon Dec 17 00:00:00 EST 2012

Galectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices.

Glycobiology

Nobumoto A,Nagahara K,Oomizu S,Katoh S,Nishi N,Takeshita K,Niki T,Tominaga A,Yamauchi A,Hirashima M

11-0491 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanism by which galectin-9 inhibits lung metastasis in experimental mouse models.

Mon Sep 01 00:00:00 EDT 2008

Basophils are essential initiators of a novel type of chronic allergic inflammation.

Blood

Obata K,Mukai K,Tsujimura Y,Ishiwata K,Kawano Y,Minegishi Y,Watanabe N,Karasuyama H

11-0491 was used in Flow cytometry/Cell sorting to study a possible role for basophils in allergic disorders and immunity to pathogens.

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: CD49B; DX5; Itga2

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UniProt ID: (Mouse) Q62469

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Entrez Gene ID: (Mouse) 16398

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Function(s)

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin

Process(es)

Performance Guarantee

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CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), FITC, eBioscience™

CD49b (Integrin alpha 2) Antibody (11-0491-82) in Flow

Product Details

11-0491-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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