Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (eBioY418 (Y418)), FITC, eBioscience™

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View all (70) CD49b (Integrin alpha 2) antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

11-0498-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioY418 (Y418)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907400

Product Specific Information

Description: The eBioY418 (Y418) monoclonal antibody reacts with human CD49b, the 150-kDa, integrin alpha 2 subunit. The complex of CD49b non-covalently associated with integrin beta 1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by lymphocytes, activated T cells, monocytes and platelets.

Applications Reported: This eBioY418 (Y418) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioY418 (Y418) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: BR; CD49B; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

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UniProt ID: (Human) P17301

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Entrez Gene ID: (Human) 3673

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Function(s)
virus receptor activity integrin binding protein binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin
Process(es)
response to hypoxia positive regulation of leukocyte migration substrate-dependent cell migration hypotonic response cell adhesion cell-matrix adhesion integrin-mediated signaling pathway female pregnancy blood coagulation cell proliferation organ morphogenesis positive regulation of epithelial cell migration positive regulation of alkaline phosphatase activity response to amine response to muscle activity positive regulation of smooth muscle cell migration extracellular matrix organization mammary gland development positive regulation of cell projection organization cell-substrate adhesion positive regulation of collagen biosynthetic process positive regulation of collagen binding response to L-ascorbic acid cell adhesion mediated by integrin collagen-activated signaling pathway response to drug positive regulation of DNA binding skin morphogenesis establishment of protein localization positive regulation of translation positive regulation of cell adhesion positive regulation of smooth muscle contraction viral entry into host cell focal adhesion assembly mesodermal cell differentiation positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of positive chemotaxis detection of mechanical stimulus involved in sensory perception of pain positive regulation of transmission of nerve impulse positive regulation of phagocytosis, engulfment hepatocyte differentiation response to parathyroid hormone cellular response to mechanical stimulus cellular response to estradiol stimulus
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