Invitrogen

CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC, eBioscience™

1 Published Figure

12 References

View all (12) CD51/CD61 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD51/CD61 (Integrin alpha v beta 3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD51/CD61 (Integrin alpha v beta 3) Antibody (11-0519-42) in Flow

Staining of the M21 cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD51/CD61 (Integrin alpha/beta 3) FITC (filled histogram). Total viable cells were used for analysis.

CD51/CD61 (Integrin alpha v beta 3) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

11-0519-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

23C6

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907402

Product Specific Information

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 23C6 antibody has been pre-titrated and tested by flow cytometric analysis of human melanoma cell line and peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGFβ-Induced EMT in Human Non-Small Cell Lung Cancer.

International journal of molecular sciences

Andreucci E,Bugatti K,Peppicelli S,Ruzzolini J,Lulli M,Calorini L,Battistini L,Zanardi F,Sartori A,Bianchini F

11-0519-42 was used in Flow cytometry/Cell sorting to highlight αvβ6 receptors as privileged access points for dual-targeting molecular conjugates engaged in an efficient and precise strategy against non-small cell lung cancer.

Thu Jan 12 00:00:00 EST 2023

Effect of Denosumab on the Change of Osteoclast Precursors Compared to Zoledronate Treatment in Postmenopausal Women with Osteoporosis.

Journal of bone metabolism

Kong SH,Kim JH,Kim SW,Jeong AJ,Lee SH,Ye SK,Shin CS

11-0519-42 was used in Flow cytometry/Cell sorting to conclude that denosumab and zoledronate treatments induced similar changes in OC precursors.

Sun May 01 00:00:00 EDT 2022

Targeting Ultrasmall Gold Nanoparticles with cRGD Peptide Increases the Uptake and Efficacy of Cytotoxic Payload.

Nanomaterials (Basel, Switzerland)

Perrins RD,McCarthy LA,Robinson A,Spry KL,Cognet V,Ferreira A,Porter J,Garc¿a CE,Rodriguez MÁ,Lopez D,Perera I,Conlon K,Barrientos A,Coulter T,Pace A,Hale SJM,Ferrari E,Bachrati CZ

11-0519-42 was used in flow cytometry to combine the versatility of ultrasmall gold nanoparticles with the target selectivity of cRGD peptide for the directed delivery of a cytotoxic payload in a novel design.

Tue Nov 15 00:00:00 EST 2022

High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.

Nanomaterials (Basel, Switzerland)

Nagaraj R,Stack T,Yi S,Mathew B,Shull KR,Scott EA,Mathew MT,Bijukumar DR

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of an anti-angiogenic peptide coating on micelle surfaces on neovascularization.

Sun Mar 22 00:00:00 EDT 2020

High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.

Nanomaterials (Basel, Switzerland)

Nagaraj R,Stack T,Yi S,Mathew B,Shull KR,Scott EA,Mathew MT,Bijukumar DR

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of an anti-angiogenic peptide coating on micelle surfaces on neovascularization.

Sun Mar 22 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.

Molecular therapy. Nucleic acids

Cen B,Wei Y,Huang W,Teng M,He S,Li J,Wang W,He G,Bai X,Liu X,Yuan Y,Pan X,Ji A

11-0519 was used in Immunocytochemistry-immunoflourescence to reveal a great translational potential of biRGD-siPIK3CB conjugate as a novel molecule for glioblastoma therapy.

Fri Dec 07 00:00:00 EST 2018

An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.

Molecular therapy. Nucleic acids

Cen B,Wei Y,Huang W,Teng M,He S,Li J,Wang W,He G,Bai X,Liu X,Yuan Y,Pan X,Ji A

11-0519 was used in Immunocytochemistry-immunoflourescence to reveal a great translational potential of biRGD-siPIK3CB conjugate as a novel molecule for glioblastoma therapy.

Fri Dec 07 00:00:00 EST 2018

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Immunology

Swindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus.

Sun Nov 01 00:00:00 EDT 2015

Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.

Human reproduction (Oxford, England)

Green CJ,Fraser ST,Day ML

11-0519 was used in Flow cytometry/Cell sorting to study an increase in apical fibronectin in blastocysts mediated by insulin-like growth factor to increase blastocyst attachment to endometrial epithelial cells in vitro.

Sun Feb 01 00:00:00 EST 2015

Tumor-targeted in vivo gene silencing via systemic delivery of cRGD-conjugated siRNA.

Nucleic acids research

Liu X,Wang W,Samarsky D,Liu L,Xu Q,Zhang W,Zhu G,Wu P,Zuo X,Deng H,Zhang J,Wu Z,Chen X,Zhao L,Qiu Z,Zhang Z,Zeng Q,Yang W,Zhang B,Ji A

11-0519 was used in Flow cytometry/Cell sorting to assess the anti-tumour potential of an siRNA molecule targetting VEGFR2 conjugated to cRGD peptide.

Wed Oct 01 00:00:00 EDT 2014

Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.

Arthritis research & therapy

Sekine C,Koyanagi A,Koyama N,Hozumi K,Chiba S,Yagita H

11-0519 was used in Flow cytometry/Cell sorting to determine the role of individual Notch receptors and ligands in osteoclastogenesis..

Mon Mar 05 00:00:00 EST 2012

Bioinformatics

Protein Aliases: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; Vitronectin receptor subunit alpha

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Gene Aliases: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR

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UniProt ID: (Human) P06756, (Human) P05106

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Entrez Gene ID: (Human) 3685, (Human) 3690

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Function(s)

virus receptor activity opsonin binding fibronectin binding protease binding protein kinase C binding voltage-gated calcium channel activity protein binding coreceptor activity fibroblast growth factor binding C-X3-C chemokine binding insulin-like growth factor I binding neuregulin binding metal ion binding transforming growth factor beta binding extracellular matrix binding extracellular matrix protein binding protein disulfide isomerase activity platelet-derived growth factor receptor binding enzyme binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding integrin

Process(es)

angiogenesis antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent cell adhesion cell-matrix adhesion integrin-mediated signaling pathway positive regulation of cell proliferation negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage cell growth cell migration extracellular matrix organization positive regulation of cell migration cell-substrate adhesion negative regulation of lipid transport positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading endodermal cell differentiation apolipoprotein A-I-mediated signaling pathway apoptotic cell clearance negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of cell adhesion viral entry into host cell vascular endothelial growth factor receptor signaling pathway negative regulation of lipoprotein metabolic process regulation of phagocytosis leukocyte migration negative chemotaxis entry of symbiont into host cell by promotion of host phagocytosis ERK1 and ERK2 cascade calcium ion transmembrane transport extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic cell clearance negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation platelet degranulation cell-substrate junction assembly blood coagulation positive regulation of endothelial cell migration smooth muscle cell migration platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway activation of protein kinase activity tube development wound healing regulation of bone resorption mesodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation angiogenesis involved in wound healing platelet aggregation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC, eBioscience™

CD51/CD61 (Integrin alpha v beta 3) Antibody (11-0519-42) in Flow

Product Details

11-0519-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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