Invitrogen

CD64 Monoclonal Antibody (10.1), FITC, eBioscience™

4 Published Figures

9 References

View all (98) CD64 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD64 Antibody (11-0649-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD64 (Fc gamma Receptor 1) FITC (purple histogram). Cells in the monocyte gate were used for analysis.

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Product Details

11-0649-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

10.1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10544395

Product Specific Information

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD64 monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Published figure using CD64 monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Published figure using CD64 monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

11-0649 was used in Flow cytometry/Cell sorting to report a disease modeling platform to study the role of macrophages in patients with inborn errors of IFN-γ immunity.

Wed Feb 19 00:00:00 EST 2020

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies.

mAbs

Hristodorov D,Mladenov R,von Felbert V,Huhn M,Fischer R,Barth S,Thepen T

11-0649 was used in Flow cytometry/Cell sorting to show that targeting M1 macrophages through CD64 can be instrumental in novel strategies for chronic inflammatory conditions.

Mon Jun 13 00:00:00 EDT 2016

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies.

mAbs

Hristodorov D,Mladenov R,von Felbert V,Huhn M,Fischer R,Barth S,Thepen T

11-0649 was used in Flow cytometry/Cell sorting to show that targeting M1 macrophages through CD64 can be instrumental in novel strategies for chronic inflammatory conditions.

Mon Jun 13 00:00:00 EDT 2016

Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy.

mAbs

Hristodorov D,Mladenov R,Brehm H,Fischer R,Barth S,Thepen T

Published figure using CD64 (Fc gamma Receptor 1) monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Immunophenotyping in myelodysplastic syndromes can add prognostic information to well-established and new clinical scores.

PloS one

Reis-Alves SC,Traina F,Harada G,Campos PM,Saad ST,Metze K,Lorand-Metze I

11-0649 was used in Flow cytometry/Cell sorting to show that immunophenotypic analysis of myelomonocytic progenitors provides additional prognostic information for clinical scores.

Mon Sep 08 00:00:00 EDT 2014

Intracellular reprogramming of expression, glycosylation, and function of a plant-derived antiviral therapeutic monoclonal antibody.

PloS one

Lee JH,Park DY,Lee KJ,Kim YK,So YK,Ryu JS,Oh SH,Han YS,Ko K,Choo YK,Park SJ,Brodzik R,Lee KK,Oh DB,Hwang KA,Koprowski H,Lee YS,Ko K

11-0649 was used in Flow cytometry/Cell sorting to show that adding an ER retention signal to a plant-derived human anti-rabies mAb expressed in tobacco plants enhances the final mAb yield.

Tue Sep 23 00:00:00 EDT 2014

The guanine nucleotide exchange protein for ADP-ribosylation factor 6, ARF-GEP100/BRAG2, regulates phagocytosis of monocytic phagocytes in an ARF6-dependent process.

The Journal of biological chemistry

Someya A,Moss J,Nagaoka I

11-0649 was used in Flow cytometry/Cell sorting to provide evidence that GEP100 functions in phagocytosis via its role in ARF6-dependent actin remodelling.

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD64; CD64 antigen; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; RP11-196G18.2

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Gene Aliases: CD64; CD64A; FCG1; FCGR1; FCGR1A; FCRI; IGFR1

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UniProt ID: (Human) P12314

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Entrez Gene ID: (Human) 2209

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin receptor superfamily

Process(es)

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent receptor-mediated endocytosis phagocytosis, engulfment immune response signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response interferon-gamma-mediated signaling pathway

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CD64 Monoclonal Antibody (10.1), FITC, eBioscience™

CD64 Antibody (11-0649-42) in Flow

Product Details

11-0649-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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