Invitrogen

CD80 (B7-1) Monoclonal Antibody (2D10.4), FITC, eBioscience™

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Antibody Testing Data (1)
Published Figures (18)

CD80 (B7-1) Antibody in Flow Cytometry (Flow)

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CD80 (B7-1) Antibody (11-0809-42) in Flow

Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.

CD80 (B7-1) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

11-0809-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 18 publications 18 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D10.4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853194

Product Specific Information

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy.

Research (Washington, D.C.)

Yang C,Ming Y,Zhou K,Hao Y,Hu D,Chu B,He X,Yang Y,Qian Z

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses.

PLoS pathogens

Mikhalkevich N,O'Carroll IP,Tkavc R,Lund K,Sukumar G,Dalgard CL,Johnson KR,Li W,Wang T,Nath A,Iordanskiy S

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

11-0809 was used in Flow cytometry/Cell sorting to assess the immunological properties of human induced pluripotent stem cells-neural crest cells.

Tue Jan 01 00:00:00 EST 2019

Astragalus polysaccharides (PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer.

Nutrients

Bamodu OA,Kuo KT,Wang CH,Huang WC,Wu ATH,Tsai JT,Lee KY,Yeh CT,Wang LS

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Fri Sep 20 00:00:00 EDT 2019

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response.

Frontiers in immunology

García-González PA,Schinnerling K,Sepúlveda-Gutiérrez A,Maggi J,Mehdi AM,Nel HJ,Pesce B,Larrondo ML,Aravena O,Molina MC,Catalán D,Thomas R,Verdugo RA,Aguillón JC

11-0809 was used in Flow cytometry/Cell sorting to study the transcriptional profile of monocyte-derived dendritic cells from healthy subjects to identify molecular regulators and pathways associated with the induction of tolerogenic properties in tolerogenic dendritic cells.

Wed Nov 20 00:00:00 EST 2019

Antigen-Presenting Human γδ T Cells Promote Intestinal CD4⁺ T Cell Expression of IL-22 and Mucosal Release of Calprotectin.

Journal of immunology (Baltimore, Md. : 1950)

Tyler CJ,McCarthy NE,Lindsay JO,Stagg AJ,Moser B,Eberl M

Mon May 01 00:00:00 EDT 2017

MALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells.

Experimental and therapeutic medicine

Wu X,Xu L,Shen Y,Yu N,Zhang Y,Guo T

11-0809 was used in Flow cytometry/Cell sorting to assess the role of TLR6 in the immune status of umbilical cord mesenchymal stem cells, by stimulation with the TLR6 agonist MALP-2.

Fri Dec 01 00:00:00 EST 2017

Immunoprofiling of Adult-Derived Human Liver Stem/Progenitor Cells: Impact of Hepatogenic Differentiation and Inflammation.

Stem cells international

El-Kehdy H,Sargiacomo C,Fayyad-Kazan M,Fayyad-Kazan H,Lombard C,Lagneaux L,Sokal E,Najar M,Najimi M

11-0809-42 was used in Flow cytometry/Cell sorting to suggest CD200 as a key factor in regulating the immunobiology of differentiated ADHLSCs.

Sat Apr 09 00:00:00 EDT 2022

CD80 down-regulation is associated to aberrant DNA methylation in non-inflammatory colon carcinogenesis.

BMC cancer

Scarpa M,Scarpa M,Castagliuolo I,Erroi F,Basato S,Brun P,Angriman I,Castoro C

11-0809 was used in Flow cytometry/Cell sorting to indicate that the failure of immune surveillance mechanisms in non-inflammatory colon carcinogenesis may affect CD80 expression.

Mon Jul 04 00:00:00 EDT 2016

The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord.

International journal of molecular medicine

Zhang L,Liu D,Pu D,Wang Y,Li L,He Y,Li Y,Li L,Qiu Z,Zhao S,Li W

11-0809 was used in Western Blotting to examine the role of the TLR3 and TLR4 pathways in modulating the immune properties of human umbilical cord-derived MSCs.

Wed Apr 01 00:00:00 EDT 2015

A DNA vaccine encoding mutated HPV58 mE6E7-Fc-GPI fusion antigen and GM-CSF and B7.1.

OncoTargets and therapy

Wang H,Yu J,Li L

Published figure using CD80 (B7-1) monoclonal antibody (Product # 11-0809-42) in Immunofluorescence

Wed Nov 25 00:00:00 EST 2015

HIV-1 and HIV-2 differentially mature plasmacytoid dendritic cells into IFN-producing cells or APCs.

Journal of immunology (Baltimore, Md. : 1950)

Royle CM,Graham DR,Sharma S,Fuchs D,Boasso A

11-0809 was used in Flow cytometry/Cell sorting to study the factors causing the observed differences in pathogenicity between HIV-1 and HIV-2.

Wed Oct 01 00:00:00 EDT 2014

Haemophilus ducreyi-induced interleukin-10 promotes a mixed M1 and M2 activation program in human macrophages.

Infection and immunity

Li W,Katz BP,Spinola SM

11-0809 was used in Flow cytometry/Cell sorting to investigate the contribution of IL-10-modulated macrophage polarisation to H. ducreyi clearance during human infection.

Sat Dec 01 00:00:00 EST 2012

Down-regulation of MHC class II expression through inhibition of CIITA transcription by lytic transactivator Zta during Epstein-Barr virus reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Li D,Qian L,Chen C,Shi M,Yu M,Hu M,Song L,Shen B,Guo N

11-0809 was used in Flow cytometry/Cell sorting to indicate that Zta may act as an inhibitor of the MHC class II pathway, suppressing CIITA transcription.

Sun Feb 15 00:00:00 EST 2009

Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.

The Journal of clinical investigation

Bashuda H,Kimikawa M,Seino K,Kato Y,Ono F,Shimizu A,Yagita H,Teraoka S,Okumura K

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1

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Gene Aliases: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; LAB7

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UniProt ID: (Human) P33681

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Entrez Gene ID: (Human) 941

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Function(s)

virus receptor activity protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

cell-cell signaling positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling T cell costimulation intracellular signal transduction T cell activation positive regulation of interleukin-2 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of T-helper 1 cell differentiation positive regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD80 (B7-1) Monoclonal Antibody (2D10.4), FITC, eBioscience™

CD80 (B7-1) Antibody (11-0809-42) in Flow

Product Details

11-0809-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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