Invitrogen

CD94 Monoclonal Antibody (DX22), FITC, eBioscience™

FIGURE: 1 / 1

CD94 Antibody (11-0949-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (dotted histogram) Anti-Human CD94 FITC (black histogram).

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Product Details

11-0949-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DX22

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149673

Product Specific Information

Description: The DX22 monoclonal antibody reacts with human CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of gd T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage.

Applications Reported: The DX22 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX22 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.

Frontiers in immunology

Creyns B,Jacobs I,Verstockt B,Cremer J,Ballet V,Vandecasteele R,Vanuytsel T,Ferrante M,Vermeire S,Van Assche G,Ceuppens JL,Breynaert C

11-0949 was used in Flow cytometry/Cell sorting to study the impact of biological treatment on innate lymphoid cells in Crohn disease and ulcerative colitis.

Mon Apr 12 00:00:00 EDT 2021

CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors.

Journal of immunology (Baltimore, Md. : 1950)

Bar-Ephraim YE,Koning JJ,Burniol Ruiz E,Konijn T,Mourits VP,Lakeman KA,Boon L,Bögels M,van Maanen JP,Den Haan JMM,van Egmond M,Bouma G,Reijmers RM,Mebius RE

11-0949-42 was used in Flow Cytometry to demonstrate that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.

Tue Jan 01 00:00:00 EST 2019

Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.

Journal of immunology (Baltimore, Md. : 1950)

Cuff AO,Robertson FP,Stegmann KA,Pallett LJ,Maini MK,Davidson BR,Male V

11-0949 was used in Flow cytometry/Cell sorting to study the molecular signal that characterises an Eomeshi population of natural killer cells in the human liver that is not present in the blood.

Thu Dec 01 00:00:00 EST 2016

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.

The Journal of clinical investigation

Peng H,Jiang X,Chen Y,Sojka DK,Wei H,Gao X,Sun R,Yokoyama WM,Tian Z

11-0949 was used in Flow cytometry/Cell sorting to define the transcriptional, phenotypic and functional features of natural killer cells in the liver; showing that liver resident CD49a+DX5- natural killer cells possess memory potential and are derived from HPCs/HSCs rather than bone marrow.

Mon Apr 01 00:00:00 EDT 2013

Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.

PloS one

Xu X,Tan C,Li P,Zhang S,Pang X,Liu H,Li L,Sun X,Zhang Y,Wu H,Chen X,Ge Q

11-0949 was used in Flow cytometry/Cell sorting to investigate the alterations in human immune cell distributions and their functions in response to various stimuli during head-down bed rest.

Tue Jun 17 00:00:00 EDT 2014

Neuron-interacting satellite glial cells in human trigeminal ganglia have an APC phenotype.

Journal of immunology (Baltimore, Md. : 1950)

van Velzen M,Laman JD,Kleinjan A,Poot A,Osterhaus AD,Verjans GM

11-0949 was used in Flow cytometry/Cell sorting to indicate a role for TG-glial cells as resident APCs with potential T cell modulatory properties.

Sat Aug 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD94; CD94 antigen; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KP43; natural killer cell receptor; Natural killer cells antigen CD94; NK cell receptor

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Gene Aliases: CD94; KLRD1

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UniProt ID: (Human) Q13241

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Entrez Gene ID: (Human) 3824

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Function(s)

transmembrane signaling receptor activity protein binding MHC class I protein complex binding MHC class Ib protein binding, via antigen binding groove carbohydrate binding protein antigen binding

Process(es)

natural killer cell mediated immunity cell surface receptor signaling pathway innate immune response regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD94 Monoclonal Antibody (DX22), FITC, eBioscience™

CD94 Antibody (11-0949-42) in Flow

Product Details

11-0949-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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