Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Mouse IgG3 Isotype Control (B10), FITC, eBioscience™
Product Details
11-4742-42
Applications
Tested Dilution
Flow Cytometry (Flow)
10 µL/test
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG3, lambda
Class
Monoclonal
Type
Isotype Control
Clone
B10
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2043894
Product Specific Information
Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG3 isotype control has been reported for use in flow cytometric analysis and ELISA.
Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 10 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. .
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G3; ImmunoglobulinG; ImmunoglobulinG3
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support