Invitrogen

Ly-49C/I/F/H Monoclonal Antibody (14B11), FITC, eBioscience™

Ly-49C/I/F/H Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Ly-49C/I/F/H Antibody (11-5991-81) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Anti-Mouse Ly-49C/I/F/H FITC (right). Total viable cells were used for analysis.

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Product Details

11-5991-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

14B11

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465339

Product Specific Information

Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.

Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.

The Journal of clinical investigation

Peng H,Jiang X,Chen Y,Sojka DK,Wei H,Gao X,Sun R,Yokoyama WM,Tian Z

11-5991 was used in Flow cytometry/Cell sorting to define the transcriptional, phenotypic and functional features of natural killer cells in the liver; showing that liver resident CD49a+DX5- natural killer cells possess memory potential and are derived from HPCs/HSCs rather than bone marrow.

Mon Apr 01 00:00:00 EDT 2013

Differential requirements for IRF-2 in generation of CD1d-independent T cells bearing NK cell receptors.

Journal of immunology (Baltimore, Md. : 1950)

Notake T,Horisawa S,Sanjo H,Miyagawa S,Hida S,Taki S

11-5991 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the generation and function of NKR(+) T cells.

Tue May 15 00:00:00 EDT 2012

A dynamic flux in natural killer cell subsets as a function of the duration of alcohol ingestion.

Alcoholism, clinical and experimental research

Ballas ZK,Cook RT,Shey MR,Coleman RA

11-5991 was used in Flow cytometry/Cell sorting to investigate the effect of duration of alcohol ingestion on NK cell function, showing a dynamic flux in NK cell subsets as a function of ingestion.

Tue May 01 00:00:00 EDT 2012

Development of murine hepatic NK cells during ontogeny: comparison with spleen NK cells.

Clinical & developmental immunology

Wu X,Chen Y,Wei H,Sun R,Tian Z

11-5991 was used in Flow cytometry/Cell sorting to investigate the phenotype of developing liver NK cells compared to spleen NK cells.

Tue Apr 24 00:00:00 EDT 2012

Distinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains.

The Journal of experimental medicine

Pyzik M,Charbonneau B,Gendron-Pontbriand EM,Babić M,Krmpotić A,Jonjić S,Vidal SM

11-5991 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection.

Mon May 09 00:00:00 EDT 2011

Interleukin-6 contributes to age-related alteration of cytokine production by macrophages.

Mediators of inflammation

Gomez CR,Karavitis J,Palmer JL,Faunce DE,Ramirez L,Nomellini V,Kovacs EJ

11-5991 was used in Flow cytometry/Cell sorting to demonstrate in vitro that Interleukin-6 regulates age-related defects in splenic macrophages through alteration of proinflammatory cytokines.

Thu Nov 04 00:00:00 EDT 2010

Unique receptor repertoire in mouse uterine NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Yadi H,Burke S,Madeja Z,Hemberger M,Moffett A,Colucci F

11-5991-81 was used in Flow Cytometry to define the receptor repertoire and phenotype of uterine NK cells at mid-gestation in mice.

Sat Nov 01 00:00:00 EDT 2008

KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice.

The Journal of clinical investigation

Parsons CH,Adang LA,Overdevest J,O'Connor CM,Taylor JR,Camerini D,Kedes DH

11-5991 was used in Flow cytometry/Cell sorting to develop an animal model for Kaposi sarcoma-associated herpesvirus (KSHV) infection.

Sat Jul 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: 5E6; cytomegalovirus resistance 1; Killer cell lectin-like receptor 3; Killer cell lectin-like receptor 6; Killer cell lectin-like receptor 8; Ly-49c; Ly-49f; Ly-49h; Lymphocyte antigen 49c; Lymphocyte antigen 49f; Lymphocyte antigen 49h; natural killer cell-associated antigen 2; Nk2.1; T-cell surface glycoprotein Ly-49C; T-cell surface glycoprotein Ly-49F; T-cell surface glycoprotein Ly-49H

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Gene Aliases: 5E6; Cmv-1; Cmv1; Klra3; Klra6; Klra8; Ly-49c; Ly-49f; Ly-49h; Ly49-f; Ly49-h; Ly49C; Ly49F; Ly49H; Ly49u<129>; Nk-2; NK-2.1; Nk2; Nk2.1

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UniProt ID: (Mouse) Q64329, (Mouse) Q60653, (Mouse) Q60682

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Entrez Gene ID: (Mouse) 16634, (Mouse) 16637, (Mouse) 16639

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Function(s)

carbohydrate binding

Process(es)

cell adhesion response to virus

Performance Guarantee

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Ly-49C/I/F/H Monoclonal Antibody (14B11), FITC, eBioscience™

Ly-49C/I/F/H Antibody (11-5991-81) in Flow

Product Details

11-5991-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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