Invitrogen

IRF4 Monoclonal Antibody (3E4), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

17 References

View all (42) IRF4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 16

IRF4 Antibody (11-9858-82) in Flow

Intracellular staining of unstimulated (orange histogram) or 3-day Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated normal human peripheral blood cells (purple histogram) with 0.06 µg of Anti-Human/Mouse IRF4 FITC using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable lymphocytes, as determined by Fixable Viability Dye eFluor® 660, were used for analysis.

Product Image Gallery

IRF4 Antibody — Fig. 8. d -gamma-Tocopherol supplementation of allergic female mice increased the numbers of CD11c + CD11b + DCs in the pup lung. The lung tissues were from the pups treated as in Fig. 2 . Lung DCs were isolated, immunolabeled, and examined by flow cytometry. A : chart of lung CD11c + subsets analyzed in the pup lungs. B : numbers of CD11b + DC subsets per million lung cells. C : numbers of CD11b - DC subsets per million lung cells. Presented is the mean +- SE; n = 8-10 mice/group. * P < 0.05, compared with basal-saline group. ** P < 0.05, compared with all other groups. {TM}

Product Details

11-9858-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 15 publications 15 publications

ELISA (ELISA)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rhesus monkey

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3E4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572538

Product Specific Information

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), Th2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported:This 3E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 3E4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.

Vaccines

Sangare K,Helmold Hait S,Moore M,Hogge C,Hoang T,Rahman MA,Venzon DJ,LaBranche C,Montefiori D,Robert-Guroff M,Thomas MA

11-9858-82 was used in Flow Cytometry to show that cells infected with an E1B55K and E4orf1-deleted (ΔE41) Ad exhibited reduced levels of phosphorylated Akt (Ser473 and Thr308)) and expressed different intrinsic innate immune cytokines from those induced in cells infected with an E4orf1-containing, ΔE1B parental Ad that exhibited elevated levels of phosphorylated Akt.

Tue Feb 15 00:00:00 EST 2022

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques.

Journal of virology

Musich T,Thovarai V,Venzon DJ,Mohanram V,Tuero I,Miller-Novak LK,Helmold Hait S,Rahman MA,Hunegnaw R,Huiting E,Yuan W,O'hUigin C,Hoang T,Sui Y,LaBranche C,Montefiori D,Bear J,Rosati M,Bissa M,Berzofsky JA,Pavlakis GN,Felber BK,Franchini G,Robert-Guroff M

11-9858-82 was used in Flow Cytometry to highlight the critical importance of considering effects of sex and the microbiome in vaccine design and evaluation.

Mon Nov 23 00:00:00 EST 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

11-9858 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 EST 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

11-9858 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 EST 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

11-9858 was used in Flow cytometry/Cell sorting to provide further insights into vaccine-induced multifaceted mucosal T cell immunity with implications in the development of vaccines against respiratorypathogens, including influenza virus and SARS-CoV-2.

Tue Sep 22 00:00:00 EDT 2020

Pro-Survival Lipid Sphingosine-1-Phosphate Metabolically Programs T Cells to Limit Anti-tumor Activity.

Cell reports

Chakraborty P,Vaena SG,Thyagarajan K,Chatterjee S,Al-Khami A,Selvam SP,Nguyen H,Kang I,Wyatt MW,Baliga U,Hedley Z,Ngang RN,Guo B,Beeson GC,Husain S,Paulos CM,Beeson CC,Zilliox MJ,Hill EG,Mehrotra M,Yu XZ,Ogretmen B,Mehrotra S

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in ELISA

Tue Aug 13 00:00:00 EDT 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in ChIP assay

Tue Oct 02 00:00:00 EDT 2018

ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.

Scientific reports

Chen W,Nyuydzefe MS,Weiss JM,Zhang J,Waksal SD,Zanin-Zhorov A

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Wed Feb 01 00:00:00 EST 2017

γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.

American journal of physiology. Lung cellular and molecular physiology

Abdala-Valencia H,Soveg F,Cook-Mills JM

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Published figure using IRF4 monoclonal antibody (Product # 11-9858-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Loss of marginal zone B-cells in SHIVSF162P4 challenged rhesus macaques despite control of viremia to low or undetectable levels in chronic infection.

Virology

Demberg T,Mohanram V,Musich T,Brocca-Cofano E,McKinnon KM,Venzon D,Robert-Guroff M

11-9858-82 was used in Flow Cytometry to conclude that MZ B-cell dysregulation and dysfunction associated with SIV/HIV infection are not readily reversible.

Thu Oct 01 00:00:00 EDT 2015

Autophagy is a critical regulator of memory CD8(+) T cell formation.

eLife

Puleston DJ,Zhang H,Powell TJ,Lipina E,Sims S,Panse I,Watson AS,Cerundolo V,Townsend AR,Klenerman P,Simon AK

11-9858 was used in Flow cytometry/Cell sorting to show that mice lacking the autophagy gene Atg7 in T cells failed to establish CD8(+) T cell memory to influenza and MCMV infection.

Tue Nov 11 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

View more View less

Gene Aliases: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

View more View less

UniProt ID: (Human) Q15306, (Mouse) Q64287

View more View less

Entrez Gene ID: (Human) 3662, (Mouse) 16364

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

Process(es)

transcription from RNA polymerase II promoter peptidyl-lysine methylation negative regulation of toll-like receptor signaling pathway T cell activation defense response to protozoan myeloid dendritic cell differentiation positive regulation of DNA binding histone H3 acetylation histone H4 acetylation positive regulation of interleukin-10 biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-4 biosynthetic process regulation of T-helper cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway T-helper 17 cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated protein methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IRF4 Monoclonal Antibody (3E4), FITC, eBioscience™

IRF4 Antibody (11-9858-82) in Flow

Product Details

11-9858-82

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!