Invitrogen

TCR gamma/delta Monoclonal Antibody (B1.1), FITC, eBioscience™

TCR gamma/delta Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

TCR gamma/delta Antibody (11-9959-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0038-80) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human gamma delta TCR FITC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

11-9959-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (4 µg)/test

View 14 publications 14 publications

Functional Assay (Functional)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B1.1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10669049

Product Specific Information

Description: The B1.1 monoclonal antibody reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection. This monoclonal can be used as a phenotypic marker for gamma delta TCR-expressing T cells.

Applications Reported: The B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (4 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.

Cell

Rosain J,Neehus AL,Manry J,Yang R,Le Pen J,Daher W,Liu Z,Chan YH,Tahuil N,Türel Ö,Bourgey M,Ogishi M,Doisne JM,Izquierdo HM,Shirasaki T,Le Voyer T,Guérin A,Bastard P,Moncada-Vélez M,Han JE,Khan T,Rapaport F,Hong SH,Cheung A,Haake K,Mindt BC,Pérez L,Philippot Q,Lee D,Zhang P,Rinchai D,Al Ali F,Ahmad Ata MM,Rahman M,Peel JN,Heissel S,Molina H,Kendir-Demirkol Y,Bailey R,Zhao S,Bohlen J,Mancini M,Seeleuthner Y,Roelens M,Lorenzo L,Soudée C,Paz MEJ,González ML,Jeljeli M,Soulier J,Romana S,L'Honneur AS,Materna M,Martínez-Barricarte R,Pochon M,Oleaga-Quintas C,Michev A,Migaud M,Lévy R,Alyanakian MA,Rozenberg F,Croft CA,Vogt G,Emile JF,Kremer L,Ma CS,Fritz JH,Lemon SM,Spaan AN,Manel N,Abel L,MacDonald MR,Boisson-Dupuis S,Marr N,Tangye SG,Di Santo JP,Zhang Q,Zhang SY,Rice CM,Béziat V,Lachmann N,Langlais D,Casanova JL,Gros P,Bustamante J

11-9959-42 was used in In vitro Assay to show that human IRF1 is essential for IFN-γ-dependent macrophagic immunity to mycobacteria, but largely redundant for IFN-α/β-dependent antiviral immunity.

Thu Feb 02 00:00:00 EST 2023

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using TCR gamma/delta monoclonal antibody (Product # 11-9959-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

γδT Cells Are Required for CD8⁺ T Cell Response to Vaccinia Viral Infection.

Frontiers in immunology

Dai R,Huang X,Yang Y

11-9959-42 was used in Flow cytometry/Cell sorting to illustrate a critical role for γδT cells in the regulation of adaptive T cell response to viral infection.

Fri Nov 26 00:00:00 EST 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using TCR gamma/delta monoclonal antibody (Product # 11-9959-42) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using TCR gamma/delta monoclonal antibody (Product # 11-9959-42) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.

Frontiers in immunology

Creyns B,Jacobs I,Verstockt B,Cremer J,Ballet V,Vandecasteele R,Vanuytsel T,Ferrante M,Vermeire S,Van Assche G,Ceuppens JL,Breynaert C

11-9959 was used in Flow cytometry/Cell sorting to study the impact of biological treatment on innate lymphoid cells in Crohn disease and ulcerative colitis.

Mon Apr 12 00:00:00 EDT 2021

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

11-9959 was used in Flow cytometry/Cell sorting to show how systemic juvenile idiopathic arthritis (sJIA) transitions from acute to chronic through the evolving Th17 polarization that begins in Tregs and progresses to T effector cells (Teffs).

Thu Mar 26 00:00:00 EDT 2020

CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors.

Journal of immunology (Baltimore, Md. : 1950)

Bar-Ephraim YE,Koning JJ,Burniol Ruiz E,Konijn T,Mourits VP,Lakeman KA,Boon L,Bögels M,van Maanen JP,Den Haan JMM,van Egmond M,Bouma G,Reijmers RM,Mebius RE

11-9959-42 was used in Flow Cytometry to demonstrate that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.

Tue Jan 01 00:00:00 EST 2019

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

11-9959-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

High Prevalence of MERS-CoV Infection in Camel Workers in Saudi Arabia.

mBio

Alshukairi AN,Zheng J,Zhao J,Nehdi A,Baharoon SA,Layqah L,Bokhari A,Al Johani SM,Samman N,Boudjelal M,Ten Eyck P,Al-Mozaini MA,Zhao J,Perlman S,Alagaili AN

11-9959 was used in Flow cytometry/Cell sorting to show that approximately 50% of camel workers in the Kingdom of Saudi Arabia and 0% of controls were previously infected.

Tue Oct 30 00:00:00 EDT 2018

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

11-9959 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

Early changes within the lymphocyte population are associated with the development of multiple organ dysfunction syndrome in trauma patients.

Critical care (London, England)

Manson J,Cole E,De'Ath HD,Vulliamy P,Meier U,Pennington D,Brohi K

11-9959 was used in Flow cytometry/Cell sorting to investigate the relationship between early lymphocyte responses and the development of MODS.

Tue Jun 07 00:00:00 EDT 2016

Evidence for Resident Memory T Cells in Rasmussen Encephalitis.

Frontiers in immunology

Owens GC,Chang JW,Huynh MN,Chirwa T,Vinters HV,Mathern GW

11-9959 was used in Flow cytometry/Cell sorting to demonstrate that a cellular immune response to an as yet unidentified antigen(s) occurs at an early stage of Rasmussen encephalitis.

Fri Mar 04 00:00:00 EST 2016

Neutrophils in antiretroviral therapy-controlled HIV demonstrate hyperactivation associated with a specific IL-17/IL-22 environment.

The Journal of allergy and clinical immunology

Campillo-Gimenez L,Casulli S,Dudoit Y,Seang S,Carcelain G,Lambert-Niclot S,Appay V,Autran B,Tubiana R,Elbim C

11-9959 was used in Flow cytometry/Cell sorting to analyse polymorphonuclear leukocyte function in ART-treated and controlled HIV-infected patients with and without inflammatory diseases.

Sat Nov 01 00:00:00 EDT 2014

Differentiation of human peripheral blood Vδ1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells.

Blood

Correia DV,Fogli M,Hudspeth K,da Silva MG,Mavilio D,Silva-Santos B

11-9959 was used in Flow cytometry/Cell sorting to implicate NKp30(+) Vδ1(+) T-cells as a novel, inducible and specialized killer lymphocyte population with potential for human cancer immunotherapy.

Thu Jul 28 00:00:00 EDT 2011

Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys.

Infection and immunity

Deetz CO,Hebbeler AM,Propp NA,Cairo C,Tikhonov I,Pauza CD

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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TCR gamma/delta Monoclonal Antibody (B1.1), FITC, eBioscience™

TCR gamma/delta Antibody (11-9959-42) in Flow

Product Details

11-9959-42

Flow Cytometry (Flow)

Functional Assay (Functional)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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