Invitrogen

CD38 Monoclonal Antibody (90), PE, eBioscience™

21 Published Figures

27 References

View all (171) CD38 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (21)

FIGURE: 1 / 22

CD38 Antibody (12-0381-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse CD38 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-0381-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465644

Product Specific Information

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported: The 90 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

12-0381-82 was used in Flow cytometry/Cell sorting to demonstrate that EWSR1 is a negative regulator of B cell responses.

Tue Aug 09 00:00:00 EDT 2022

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

12-0381-82 was used in Flow cytometry/Cell sorting to demonstrate that EWSR1 is a negative regulator of B cell responses.

Tue Aug 09 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Sun May 30 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Vogel AB,Kanevsky I,Che Y,Swanson KA,Muik A,Vormehr M,Kranz LM,Walzer KC,Hein S,Güler A,Loschko J,Maddur MS,Ota-Setlik A,Tompkins K,Cole J,Lui BG,Ziegenhals T,Plaschke A,Eisel D,Dany SC,Fesser S,Erbar S,Bates F,Schneider D,Jesionek B,Sänger B,Wallisch AK,Feuchter Y,Junginger H,Krumm SA,Heinen AP,Adams-Quack P,Schlereth J,Schille S,Kröner C,de la Caridad Güimil Garcia R,Hiller T,Fischer L,Sellers RS,Choudhary S,Gonzalez O,Vascotto F,Gutman MR,Fontenot JA,Hall-Ursone S,Brasky K,Griffor MC,Han S,Su AAH,Lees JA,Nedoma NL,Mashalidis EH,Sahasrabudhe PV,Tan CY,Pavliakova D,Singh G,Fontes-Garfias C,Pride M,Scully IL,Ciolino T,Obregon J,Gazi M,Carrion R,Alfson KJ,Kalina WV,Kaushal D,Shi PY,Klamp T,Rosenbaum C,Kuhn AN,Türeci Ö,Dormitzer PR,Jansen KU,Sahin U

12-0381-82 was used in flow cytometry to report the preclinical development of two vaccine candidates (BNT162b1 and BNT162b2) that contain nucleoside-modified messenger RNA that encodes immunogens derived from the spike glycoprotein (S) of SARS-CoV-2, formulated in lipid nanoparticles.

Thu Apr 01 00:00:00 EDT 2021

CD38 in cancer-associated fibroblasts promotes pro-tumoral activity.

Laboratory investigation; a journal of technical methods and pathology

Ben Baruch B,Mantsur E,Franco-Barraza J,Blacher E,Cukierman E,Stein R

12-0381 was used in Flow cytometry/Cell sorting to investigate the role of CD38 in regulating cancer-associated fibroblasts pro-tumorigenic functions.

Tue Dec 01 00:00:00 EST 2020

CHK1 dosage in germinal center B cells controls humoral immunity.

Cell death and differentiation

Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

12-0381-82 was used in Flow cytometry/Cell sorting to show that the subpopulation-specific profiles of gene expression, chromatin accessibility and enhancer-promoter contact obtained by DC3 provide a comprehensive characterization of the gene regulatory system in each subpopulation.

Thu Oct 10 00:00:00 EDT 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

Thu Oct 10 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

12-0381-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

T-Bet⁺ IgM Memory Cells Generate Multi-lineage Effector B Cells.

Cell reports

Kenderes KJ,Levack RC,Papillion AM,Cabrera-Martinez B,Dishaw LM,Winslow GM

12-0381 was used in Flow cytometry/Cell sorting to propose that T-bet+ IgM memory cells can differentiate into any effector B cell lineage to provide long-term multifunctional immunity.

Tue Jul 24 00:00:00 EDT 2018

High-affinity IgM⁺ memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.

Scientific reports

Tashiro Y,Murakami A,Hara Y,Shimizu T,Kubo M,Goitsuka R,Kishimoto H,Azuma T

12-0381 was used in Flow cytometry/Cell sorting to demonstrate that only non-germinal centre-dependent IgM+ memomry B cells have terminal differential potential to become plasma cell during recall immune response.

Fri Sep 28 00:00:00 EDT 2018

Overexpression of Interleukin-7 Extends the Humoral Immune Response Induced by Rabies Vaccination.

Journal of virology

Li Y,Zhou M,Luo Z,Zhang Y,Cui M,Chen H,Fu ZF,Zhao L

12-0381-82 was used in Flow Cytometry to study primary and secondary antibody immune responses post-recombinant rabies virus immunization.

Sat Apr 01 00:00:00 EDT 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

Clonal relationships of CSF B cells in treatment-naive multiple sclerosis patients.

JCI insight

Eggers EL,Michel BA,Wu H,Wang SZ,Bevan CJ,Abounasr A,Pierson NS,Bischof A,Kazer M,Leitner E,Greenfield AL,Demuth S,Wilson MR,Henry RG,Cree BA,Hauser SL,von Büdingen HC

12-0381 was used in Flow cytometry/Cell sorting to find increased CSF lymphocytes with a disproportionate increase of B cells compared with T cells in patients with gadolinium-enhancing (Gd+) lesions on brain MRI.

Thu Nov 16 00:00:00 EST 2017

Robust growth of avirulent phase II Coxiella burnetii in bone marrow-derived murine macrophages.

PloS one

Cockrell DC,Long CM,Robertson SJ,Shannon JG,Miller HE,Myers L,Larson CL,Starr T,Beare PA,Heinzen RA

12038182 was used in flow cytometry to develop and describe reagents to study Coxiella-macrophage interactions

Wed Sep 06 00:00:00 EDT 2017

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Meningeal Infiltration of the Spinal Cord by Non-Classically Activated B Cells is Associated with Chronic Disease Course in a Spontaneous B Cell-Dependent Model of CNS Autoimmune Disease.

Frontiers in immunology

Dang AK,Tesfagiorgis Y,Jain RW,Craig HC,Kerfoot SM

12-0381 was used in Flow cytometry/Cell sorting to characterise B cell infiltration of the spinal cord in a B cell-dependent spontaneous model of CNS autoimmunity, showing meningeal infiltration of the spinal cord by non-classically activated B cells.

Wed Oct 07 00:00:00 EDT 2015

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

PP4 is essential for germinal center formation and class switch recombination in mice.

PloS one

Chen MY,Chen YP,Wu MS,Yu GY,Lin WJ,Tan TH,Su YW

12-0381 was used in Flow cytometry/Cell sorting to elucidate the role of the phosphatase PP4 in mature B cells.

Tue Dec 15 00:00:00 EST 2015

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD38 monoclonal antibody (Product # 12-0381-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

B cell activating factor inhibition impairs bacterial immunity by reducing T cell-independent IgM secretion.

Infection and immunity

Jones DD,Jones M,DeIulio GA,Racine R,MacNamara KC,Winslow GM

12-0381 was used in Flow cytometry/Cell sorting to determine that BAFF signalling can directly regulate secretory processes.

Sun Dec 01 00:00:00 EST 2013

Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response.

Blood

Gardam S,Turner VM,Anderton H,Limaye S,Basten A,Koentgen F,Vaux DL,Silke J,Brink R

12-0381 was used in Flow cytometry/Cell sorting to investigate the roles of cIAP1 and cIAP2 in controlling B-cell physiology.

Thu Apr 14 00:00:00 EDT 2011

Local renal autoantibody production in lupus nephritis.

Journal of the American Society of Nephrology : JASN

Espeli M,Bökers S,Giannico G,Dickinson HA,Bardsley V,Fogo AB,Smith KG

12-0381 was used in Flow cytometry/Cell sorting to identify the kidney as a major reservoir of autoreactive plasma cells in murine models of lupus nephritis that may contribute significantly to disease progression.

Tue Feb 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen p45; Cyclic ADP-ribose hydrolase 1; I-19; NAD+ nucleosidase; NIM-R5 antigen

View more View less

Gene Aliases: ADPRC 1; Cd38; Cd38-rs1

View more View less

UniProt ID: (Mouse) P56528

View more View less

Entrez Gene ID: (Mouse) 12494

View more View less

Function(s)

NAD+ nucleosidase activity transferase activity hydrolase activity hydrolase activity, acting on glycosyl bonds phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia positive regulation of cytosolic calcium ion concentration metabolic process response to hormone positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD38 Monoclonal Antibody (90), PE, eBioscience™

CD38 Antibody (12-0381-82) in Flow

Product Details

12-0381-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!