Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), PE, eBioscience™

2 Published Figures

16 References

View all (56) CD49d (Integrin alpha 4) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD49d (Integrin alpha 4) Antibody (12-0499-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD49d (Integrin alpha 4) PE (filled histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-0499-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

9F10

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717245

Product Specific Information

Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).

Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 9F10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Mesenchymal stem cell aggregation mediated by integrin α4/VCAM-1 after intrathecal transplantation in MCAO rats.

Stem cell research & therapy

Ran Y,Dong Y,Li Y,Xie J,Zeng S,Liang C,Dai W,Tang W,Wu Y,Yu S

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 12-0499-42) in Flow Cytometry

Sat Oct 22 00:00:00 EDT 2022

Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.

Communications biology

Soboleva S,Kurita R,Ek F,Åkerstrand H,Silvério-Alves R,Olsson R,Nakamura Y,Miharada K

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 12-0499-42) in Flow Cytometry

Thu Jun 03 00:00:00 EDT 2021

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

12-0499 was used in Flow cytometry/Cell sorting to engineer mouse and human red blood cells expressing surface single-domain antibodies against botulinum neurotoxin A, conferring long lasting protection.

Mon Sep 04 00:00:00 EDT 2017

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Immunology

Swindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD

12-0499 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus.

Sun Nov 01 00:00:00 EDT 2015

Species-specific differences in the expression and regulation of α4β7 integrin in various nonhuman primates.

Journal of immunology (Baltimore, Md. : 1950)

Byrareddy SN,Sidell N,Arthos J,Cicala C,Zhao C,Little DM,Dunbar P,Yang GX,Pierzchalski K,Kane MA,Mayne AE,Song B,Soares MA,Villinger F,Fauci AS,Ansari AA

12049942 was used in flow cytometry to analyze the expression of alpha4beta7-integrin in primate lymphocites.

Mon Jun 15 00:00:00 EDT 2015

Vitamins A and D have antagonistic effects on expression of effector cytokines and gut-homing integrin in human innate lymphoid cells.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Ruiter B,Patil SU,Shreffler WG

12-0499 was used in Flow cytometry/Cell sorting to investigate the effects of retinoic acid and the active form of vitamin D, 1,25-dihydroxyvitamin D3, on human innate lymphoid cells.

Wed Jul 01 00:00:00 EDT 2015

Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers.

Molecular & cellular proteomics : MCP

Bing T,Shangguan D,Wang Y

12-0499 was used in Flow cytometry/Cell sorting to develop a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers, for future cancer biomarker discovery.

Thu Oct 01 00:00:00 EDT 2015

Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients.

Journal of immunology (Baltimore, Md. : 1950)

Achour A,Baychelier F,Besson C,Arnoux A,Marty M,Hannoun L,Samuel D,Debré P,Vieillard V

12-0499 was used in Flow cytometry/Cell sorting to study the relationship between the modulation of natural killer cells and cytomegalovirus status after liver transplant.

Wed Jan 01 00:00:00 EST 2014

Lineage depletion of stromal vascular fractions isolated from human adipose tissue: a novel approach towards cell enrichment technology.

Cytotechnology

Indumathi S,Mishra R,Harikrishnan R,Rajkumar JS,Kantawala N,Dhanasekaran M

12-0499 was used in Flow cytometry/Cell sorting to understand the comparative efficacy of lineage depleted adipose derived stromal vascular fraction (SVF), to help rationalise tissue repair and regeneration using stem cells.

Sat Mar 01 00:00:00 EST 2014

Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.

PloS one

Spring FA,Griffiths RE,Mankelow TJ,Agnew C,Parsons SF,Chasis JA,Anstee DJ

12-0499 was used in Flow cytometry/Cell sorting to investigate whether any tetraspanin could modulate integrin function, showing that tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to VCAM-1.

Mon Dec 30 00:00:00 EST 2013

Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.

PloS one

Spring FA,Griffiths RE,Mankelow TJ,Agnew C,Parsons SF,Chasis JA,Anstee DJ

Mon Dec 30 00:00:00 EST 2013

Suppression of tumour-specific CD4⁺ T cells by regulatory T cells is associated with progression of human colorectal cancer.

Gut

Betts G,Jones E,Junaid S,El-Shanawany T,Scurr M,Mizen P,Kumar M,Jones S,Rees B,Williams G,Gallimore A,Godkin A

12-0499 was used in Flow cytometry/Cell sorting to demonstrate that CRC presence drives the activity of Tregs and the suppression of CD4+ T-cell responses to tumour-associated antigens.

Wed Aug 01 00:00:00 EDT 2012

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow.

Cytotechnology

Dhanasekaran M,Indumathi S,Kanmani A,Poojitha R,Revathy KM,Rajkumar JS,Sudarsanam D

12-0499 was used in Flow cytometry/Cell sorting to investigate surface antigenic profiling of stem cells from human omentum fat, compared with subcutaneous fat and bone marrow..

Wed Feb 01 00:00:00 EST 2012

The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.

PloS one

Lind A,Siersema PD,Kusters JG,Van der Linden JA,Knol EF,Koenderman L

12-0499 was used in Flow cytometry/Cell sorting to study how the immune cell composition (lymphocytes and eosinophils) and expression of intestinal adhesion molecule MAdCAM-1 is similar in BE and duodenum.

Mon Aug 20 00:00:00 EDT 2012

Bortezomib overcomes cell-adhesion-mediated drug resistance through downregulation of VLA-4 expression in multiple myeloma.

Oncogene

Noborio-Hatano K,Kikuchi J,Takatoku M,Shimizu R,Wada T,Ueda M,Nobuyoshi M,Oh I,Sato K,Suzuki T,Ozaki K,Mori M,Nagai T,Muroi K,Kano Y,Furukawa Y,Ozawa K

12-0499 was used in Flow cytometry/Cell sorting to identify the adhesion molecules responsible for cell adhesion-mediated drug resistance in multiple myeloma.

Thu Jan 15 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; ITGA-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

View more View less

Gene Aliases: CD49D; IA4; ITGA4

View more View less

UniProt ID: (Human) P13612

View more View less

Entrez Gene ID: (Human) 3676

View more View less

Function(s)

fibronectin binding protein binding coreceptor activity C-X3-C chemokine binding metal ion binding cell adhesion molecule binding protein antigen binding integrin

Process(es)

cell-matrix adhesion involved in ameboidal cell migration leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway B cell differentiation extracellular matrix organization heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading endodermal cell differentiation receptor clustering regulation of immune response leukocyte migration leukocyte tethering or rolling diapedesis cellular response to cytokine stimulus negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte tethering or rolling clathrin-mediated extracellular exosome endocytosis positive regulation of T cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), PE, eBioscience™

CD49d (Integrin alpha 4) Antibody (12-0499-42) in Flow

Product Details

12-0499-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!